Reduction of Aedes aegypti Vector Competence for Dengue Virus under Large Temperature Fluctuations

Lauren B. Carrington Department of Entomology, University of California, Davis, Califronia; Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris, France; Fogarty International Center, National Institutes of Health, Bethesda, Maryland

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Stephanie N. Seifert Department of Entomology, University of California, Davis, Califronia; Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris, France; Fogarty International Center, National Institutes of Health, Bethesda, Maryland

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M. Veronica Armijos Department of Entomology, University of California, Davis, Califronia; Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris, France; Fogarty International Center, National Institutes of Health, Bethesda, Maryland

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Louis Lambrechts Department of Entomology, University of California, Davis, Califronia; Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris, France; Fogarty International Center, National Institutes of Health, Bethesda, Maryland

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Thomas W. Scott Department of Entomology, University of California, Davis, Califronia; Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris, France; Fogarty International Center, National Institutes of Health, Bethesda, Maryland

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Diurnal temperature fluctuations can fundamentally alter mosquito biology and mosquito-virus interactions in ways that impact pathogen transmission. We investigated the effect of two daily fluctuating temperature profiles on Aedes aegypti vector competence for dengue virus (DENV) serotype-1. A large diurnal temperature range of 18.6°C around a 26°C mean, corresponding with the low DENV transmission season in northwestern Thailand, reduced midgut infection rates and tended to extend the virus extrinsic incubation period. Dissemination was first observed at day 7 under small fluctuations (7.6°C; corresponding with high DENV transmission) and constant control temperature, but not until Day 11 for the large diurnal temperature range. Results indicate that female Ae. aegypti in northwest Thailand are less likely to transmit DENV during the low than high transmission season because of reduced DENV susceptibility and extended virus extrinsic incubation period. Better understanding of DENV transmission dynamics will come with improved knowledge of temperature effects on mosquito-virus interactions.

Author Notes

* Address correspondence to Lauren B. Carrington, Department of Entomology, University of California, 1 Shields Avenue, Davis, CA 95616. E-mail: lbcarrington@ucdavis.edu

Financial support: The study was supported by grant EF-0914384 from the Ecology of Infectious Disease Program of the National Science Foundation. Thomas W. Scott acknowledges funding from the Research and Policy for Infectious Disease Dynamics Program.

Authors' addresses: Lauren B. Carrington, Nossal Institute for Global Health, University of Melbourne, Carlton, VIC, Australia, E-mail: lbcarrington@gmail.com. M. Veronica Armijos, Department of Entomology, University of California, Davis, CA, E-mail: mvarmijos@ucdavis.edu. Stephanie N. Seifert, Department of Biology, University of Pennsylvania, Philadelphia, PA, E-mail: seifst@sas.upenn.edu. Louis Lambrechts, Insects and Infectious Diseases, Centre National de la Recherche Scientifique, Unite de Recherche Associee 3012, Institut Pasteur, Paris Cedex 15, France, E-mail: louis.lambrechts@pasteur.fr. Thomas W. Scott, Department of Entomology, University of California, Davis, CA, and Fogarty International Center, National Institutes of Health, Bethesda, MD, E-mail: twscott@ucdavis.edu.

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