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We describe the diagnostic difficulties experienced during an opisthorchiasis outbreak. Of 31 infected individuals, 61.3% were asymptomatic, and in the 12 symptomatic individuals, the duration of non-pathognomonic symptoms was shorter than 4 weeks. Serology by enzyme-linked immunosorbent assay and polymerase chain reaction fecal analysis were shown to be the most sensitive diagnostic tools.
Authors' addresses: Orlando Armignacco, Fabrizio Ferri, and Luciano Caterini, Unitá Operativa Complessa Malattie Infettive, Belcolle Hospital, Viterbo, Italy, E-mails: armignacco@asl.vt.it, faberferri@gmail.com, and lucianocaterini@libero.it. Maria Angeles Gomez-Morales and Edoardo Pozio, Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, Rome, Italy, E-mails: mariaangeles.gomezmorales@iss.it and edoardo.pozio@iss.it.