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A cross-sectional study of Blastocystis infection was conducted to evaluate the prevalence, risk factors, and subtypes of Blastocystis at the Home for Girls, Bangkok, Thailand in November 2008. Of 370 stool samples, 118 (31.9%) were infected with Blastocystis. Genotypic characterization of Blastocystis was performed by polymerase chain reaction and sequence analysis of the partial small subunit ribosomal RNA (SSU rRNA) gene. Subtype 1 was the most predominant (94.8%), followed by subtype 6 (3.5%) and subtype 2 (1.7%). Sequence analyses revealed nucleotide polymorphisms for Blastocystis subtype 1, which were described as subtype 1/variant 1, subtype 1/variant 2. Blastocystis subtype 1/variant 1 was the most predominant infection occurring in almost every house. The results showed that subtype analysis of Blastocystis was useful for molecular epidemiological study.
Financial support: This work was supported by the Phramongkutklao Research Fund, Phramongkutklao College of Medicine, Thailand, Faculty of Science, Burapha University, Thailand, and The Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0236/2548).
Authors' addresses: Umaporn Thathaisong, Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand, E-mail: email@example.com. Suradej Siripattanapipong, Mathirut Mungthin, Tawee Naaglor, and Saovanee Leelayoova, Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand, E-mails: firstname.lastname@example.org, email@example.com, firstname.lastname@example.org, and email@example.com. Duangnate Pipatsatitpong, Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Khlong Luang, Pathum Thani, Thailand, E-mail: firstname.lastname@example.org. Peerapan Tan-ariya, Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand, E-mail: email@example.com.