Cross-Sectional Study of Leptospira Seroprevalence in Humans, Rats, Mice, and Dogs in a Main Tropical Sea-Port City

Claudia M. E. Romero-Vivas Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Margarett Cuello-Pérez Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Piedad Agudelo-Flórez Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Dorothy Thiry Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Paul N. Levett Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Andrew K. I. Falconar Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, División Ciencias de la Salud, Universidad del Norte, Barranquilla, Colombia; Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia; Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

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Samples were collected from 128 symptomatic humans, 83 dogs, 49 mice, and 20 rats (Rattus rattus: 16; Rattus norvegicus: 4) in neighborhoods where human leptospirosis have been reported within the principal sea-port city of Colombia. Seroprevalences were assessed against 19 pathogenic, 1 intermediate pathogenic, and 1 saprophytic Leptospira serogroups. Pathogenic Leptospira were confirmed using conventional Leptospira-specific polymerase chain-reaction and pulsed-field gel electrophoresis analysis was used for serovar identification. Seroprevalences of 20.4%, 12.5%, 25.0%, 22.9%, and 12.4% were obtained against one to seven different serogroups in mice, R. rattus, R. norvegicus, dogs, and humans, respectively. The DNA was confirmed to be from pathogenic Leptospira by detecting the lipL32 gene in 12.5%, 3.7%, and 0.03% of the R. rattus, dog, and human samples, respectively. The first genetically typed Colombian isolate was obtained from a rat and identified as Leptospira interrogans serovar Icterohaemorrhagiae/Copenhageni.

Author Notes

* Address correspondence to Claudia M. E. Romero-Vivas, Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, Universidad del Norte, Km 5 antigua via a Puerto Colombia, Barranquilla, Atlantico, Colombia 1. E-mail: clromero@uninorte.edu.co

Financial support: This study was supported by the Departamento Administrativo de Ciencia Tecnología e Innovación, Colciencias ID Project Number 1215-408-20551.

Authors' addresses: Claudia M. E. Romero-Vivas, Margarett Cuello-Pérez, and Andrew K. I. Falconar, Grupo de Investigaciones en Enfermedades Tropicales, Departamento de Medicina, Universidad del Norte, Barranquilla, Colombia. E-mails: clromero@uninorte.edu.co, margarethc@uninorte.edu.co, and afalconar@uninorte.edu.co. Piedad Agudelo-Flórez, Instituto Colombiano de Medicina Tropical, Universidad CES, Medellín, Colombia, E-mail: pagudelo@ces.edu.co. Dorothy Thiry and Paul N. Levett, Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada, E-mails: dthiry@health.gov.sk.ca and plevett@health.gov.sk.ca.

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