- Introduction
- Materials and Methods
- Results
- Newly selected repetitive genomic sequences of S. haematobium.
- PCR assays employing Sh73 and Sh77 primers.
- PCR assays employing combinations of primers for identifying inter-repeat sequences.
- Detection of S. haematobium and S. bovis in snail tissues.
- PCR assay with DNA from different schistosome species belonging to the S. haematobium group (species with terminal spined ova).
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
WHO, 2002. Prevention and control of schistosomiasis and soil transmitted helminthiases: report of a WHO expert committee. World Health Organ Tech Rep Ser 912: 2–3.
-
2.
Hamburger J, He N, Abbasi I, Ramzy RM, Jourdane J, Ruppel A, 2001. Polymerase chain reaction assay based on highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome infested water. Am J Trop Med Hyg 65: 907–911.
-
3.
Hamburger J, Hoffman O, Kariuki HC, Muchiri EM, Ouma JH, Koech DK, Sturrock RF, King CH, 2004. Large-scale, polymerase chain reaction-based surveillance of Schistosoma haematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Am J Trop Med Hyg 71: 765–773.
-
4.
Christensen NO, Mutani A, Frandsen F, 1983. A review of the biology and transmission ecology of African bovine species of the genus Schistosoma. Z Parasitenkd 69: 551–570.
-
5.
Mone H, Mowahid G, Monard S, 2000. The distribution of Schistosoma bovis Sonsino, 1876 in relation to intermediate host mollusc-parasite relationship. Adv Parasitol 44: 100–138.
-
6.
King CH, Sturrock RF, Kariuki HC, Hamburger J, 2006. Transmission control for schistosomiasis-why it matters now. Trends Parasitol 22: 575–582.
-
7.
Young ND, Jex AR, Li B, Liu S, Yang L, Xiong Z, Li Y, Cantacessi C, Hall RS, Xu X, Chen F, Wu X, Zerlotini A, Oliveira G, Hofmann A, Zhang G, Fang X, Kang Y, Campbell BE, Loukas A, Ranganathan S, Rollinson D, Rinaldi G, Brindley PJ, Yang H, Wang J, Wang J, Gasser RB, 2012. Whole-genome sequence of Schistosoma haematobium. Nat Genet 44: 221–225.
-
8.
Abbasi I, King CH, Sturrock RF, Kariuki HC, Muchiri E, Hamburger J, 2007. Differentiation of Schistosoma haematobium from related schistosomes by PCR amplifying an inter-repeat sequence. Am J Trop Med Hyg 76: 950–955.
-
9.
Webster BL, Rollinson D, Stothard JR, Huyse T, 2010. Rapid diagnostic multiplex PCR (RD-PCR) to discriminate Schistosoma haematobium and S. bovis. J Helminthol 84: 107–114.
-
10.
Huyse T, Webster BL, Geldof S, Stothard RJ, Diaw OT, Polman K, Rollinson D, 2009. Bidirectional introgressive hybridization between a cattle and human schistosome species. PLoS Pathog 5: e1000571.
-
11.
Jourdane J, Southgate VR, Pages JR, Durand P, Tchuem-Tchuente LA, 2001. Recent studies on Schistosoma intercalatum: taxonomic status, puzzling distribution, and transmission foci revised. Mem Inst Oswaldo Cruz 96: 45–48.
-
12.
Vercruysse J, Southgate VR, Rollinson D, DeClerq D, Sacko M, De Bont J, Mungomba LM, 1994. Studies on transmission and schistosome interactions in Senegal, Mali, and Zambia. Trop Geogr Med 46: 220–226.
-
13.
Xu J, Rong R, Zang HQ, Shi CJ, Zhu XQ, Xia CM, 2010. Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP). Int J Parasitol 40: 327–331.
-
14.
Abbasi I, King CH, Muchiri EM, Hamburger J, 2010. Detection of Schistosoma mansoni and Schistosoma haematobium DNA by loop-mediated isothermal amplification: identification of infected snails from early prepatency. Am J Trop Med Hyg 83: 427–432.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 362 | 111 | 1 |
| PDF Downloads | 92 | 20 | 2 |
Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences
In schistosomiasis elimination programs, successful discrimination of Schistosoma haematobium from the related animal Schistosoma parasites will be essential for accurate detection of human parasite transmission. Polymerase chain reaction assays employing primers from two newly selected repeated sequences, named Sh73 and Sh77, did not discriminate S. haematobium when amplifying Sh73-77 intra- or inter-repeats. However, amplification between Sh73 and the previously described DraI repeat exhibited discriminative banding patterns for S. haematobium and Schistosoma bovis (sensitivity 1 pg and 10 pg, respectively). It also enabled banding pattern discrimination of Schistosoma curassoni and Schistosoma intercalatum, but Schistosoma mattheei and Schistosoma margrebowiei did not yield amplicons. Similar inter-repeat amplification between Sh77 and DraI yielded amplicons with discriminative banding for S. haematobium, and S. bovis; however, S. mattheei was detected only at low sensitivity (1 ng). The Sh73/DraI assay detected snails infected with S. haematobium, S. bovis, or both, and should prove useful for screening snails where discrimination of S. haematobium from related schistosomes is required.
Author Notes
Financial support: This research was supported by grants from the U.S. National Institutes of Health: R21AI076672 from the National Institute of Allergy and Infectious Diseases, and R01TW008067 from the Fogarty International Center through the National Institutes of Health-National Science Foundation Ecology of Infectious Diseases Program.
Authors' addresses: Ibrahim Abbasi and Joseph Hamburger, Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel, E-mails: ibrahima@ekmd.huji.ac.il and hambu@cc.huji.ac.il. Curtis Kariuki and Eric M. Muchiri, Division of Vector Borne and Neglected Tropical Diseases, Ministry of Public Health and Sanitation, E-mails: hckariuki@yahoo.com and Ericmmuchiri@gmail.com. Peter L. Mungai, Division of Vector Borne Neglected and Tropical Diseases, Filariasis-Schistosomiasis Research Unit, Case Western Reserve University, Cleveland, OH, E-mail: plmungai@yahoo.com. Charles H. King, Center for Global Health and Diseases, CWRU School of Medicine, Cleveland, OH, E-mail: chk@cwru.edu.