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The Development of a Loop-Mediated Isothermal Amplification Method (LAMP) for Echinococcus granulosis Coprodetection

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  • Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel

We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare.

Author Notes

* Address correspondence to Harold Salant, Department of Molecular Genetics, Hebrew University-Hadassah Medical School, PO Box 12272, Jerusalem 91120, Israel. E-mail: tsvis@ekmd.huji.ac.il

Authors' addresses: Harold Salant, Ibrahim Abbasi, and Joseph Hamburger, Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel, E-mails: tsvis@ekmd.huji.ac.il, ibrahima@ekmd.huji.ac.il, and hambu@cc.huji.ac.il.

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