Molecular Identification of a Case of Paragonimus pseudoheterotremus Infection in Thailand

Pewpan M. Intapan Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Oranuch Sanpool Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Tongjit Thanchomnang Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Kanokwan Imtawil Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Chatlert Pongchaiyakul Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Yukifumi Nawa Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Wanchai Maleewong Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Department of Biochemistry, Department of Medicine, and Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand

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Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. In Southeast Asia, Paragonimus heterotremus is the only proven causative pathogen. Recently, a new Paragonimus species, P. pseudoheterotremus, was found in Thailand. This species is genetically similar to P. heterotremus and is considered as a sister species. However, infectivity or pathogenicity of P. pseudoheterotremus to humans remains unclear. We report the first confirmed human pulmonary paragonimiasis case caused by P. pseudoheterotremus infection. After polymerase chain reaction/sequencing of the DNA extracted from Paragonimus eggs in the sputum of the patient, partial internal transcribed spacer 2 and cytochrome c oxidase subunit 1 sequences were approximately identical (98–100%) with those of P. pseudoheterotremus. For P. heterotremus, the partial internal transcribed spacer 2 sequence was approximately identical (99–100%), but the partial mitochondrial cytochrome c oxidase subunit 1 sequence showed a similarity of 90–95%.

Author Notes

*Address correspondence to Pewpan M. Intapan, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan@kku.ac.th

Financial support: This study was supported by the National Science and Technology Development Agency (Discovery Based Development Grant), the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, and the Faculty of Medicine, Khon Kaen University, Thailand.

Authors' addresses: Pewpan M. Intapan, Oranuch Sanpool, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen 40002, Thailand, E-mails: pewpan@kku.ac.th, chimy_6891@hotmail.com, and wanch_ma@kku.ac.th. Tongjit Thanchomnang, Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand, E-mail: thanchomnang@yahoo.com. Kanokwan Imtawil, Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: kanjan@kku.ac.th. Chatlert Pongchaiyakul, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: pchatl@kku.ac.th. Yukifumi Nawa, Research Division, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: yukinawa@kku.ac.th.

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