Genetic Characterization of Atypical Mansonella (Mansonella) ozzardi Microfilariae in Human Blood Samples from Northeastern Peru

Luis A. Marcos Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Nancy Arrospide Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Sergio Recuenco Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Cesar Cabezas Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Gary J. Weil Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Peter U. Fischer Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru; Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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DNA sequence comparisons are useful for characterizing proposed new parasite species or strains. Microfilariae with an atypical arrangement of nuclei behind the cephalic space have been recently described in human blood samples from the Amazon region of Peru. Three blood specimens containing atypical microfilariae were genetically characterized using three DNA markers (5S ribosomal DNA, 12S ribosomal DNA, and cytochrome oxidase I). All atypical microfilariae were clustered into the Mansonella group and indistinguishable from M. ozzardi based on these DNA markers.

Author Notes

* Address correspondence to Peter U. Fischer, Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110. E-mail: Pufische@DOM.Wustl.edu

Financial support: This study was supported by a grant from the Barnes Jewish Hospital Foundation.

Authors' addresses: Luis A. Marcos, Gary J. Weil, and Peter U. Fischer, Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, E-mails: marcoslrz@yahoo.com, GWeil@DOM.wustl.edu, and Pufische@DOM.wustl.edu. Nancy Arrospide and Cesar Cabezas, Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru, E-mails: narrospide@hotmail.com and ccabezas@ins.gob.pe. Sergio Recuenco, Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases and Prevention, Atlanta, GA, E-mail: fni9@cdc.gov.

  • 1.

    Eberhard ML, Orihel TC, 1984. The genus Mansonella (Syn. Tetrapetalonema). A new classification. Ann Parasitol Hum Comp 59: 483496.

  • 2.

    Kazura JW, 1999. Filariasis. Guerrant RL, Walker DH, Weller PF, eds. Tropical Infectious Diseases: Principles, Pathogens, & Practice, 1st ed. Philadelphia, PA: Churchill Livingstone, 852869.

    • Search Google Scholar
    • Export Citation
  • 3.

    Godoy G, Orihel T, Volcan G, 1980. Microfilaria bolivarensis: a new species of filaria from man in Venezuela. Am J Trop Med Hyg 29: 545547.

    • Search Google Scholar
    • Export Citation
  • 4.

    Adami YL, Moraes AP, Lanfredi RM, Maia-Herzog M, 2008. An atypical microfilaria in blood samples from inhabitants of Brazilian Amazon. Parasitol Res 104: 9599.

    • Search Google Scholar
    • Export Citation
  • 5.

    Arrospide N, Adami YL, Durand S, Rimarachin D, Gutierrez S, Cabezas C, 2009. Atypical microfilaria in coinfection with Mansonella ozzardi and Plasmodium vivax in Peruvian amazon. Rev Peru Med Exp Salud Publica 26: 408416.

    • Search Google Scholar
    • Export Citation
  • 6.

    Shelley AJ, 2002. Human onchocerciasis in Brazil: an overview. Cad Saude Publica 18: 11671177.

  • 7.

    Orihel TC, Eberhard ML, 1982. Mansonella ozzardi: a redescription with comments on its taxonomic relationships. Am J Trop Med Hyg 31: 11421147.

    • Search Google Scholar
    • Export Citation
  • 8.

    Xie H, Bain O, Williams SA, 1994. Molecular phylogenetic studies on filarial parasites based on 5S ribosomal spacer sequences. Parasite 1: 141151.

    • Search Google Scholar
    • Export Citation
  • 9.

    Casiraghi M, Bain O, Guerrero R, Martin C, Pocacqua V, Gardner SL, Franceschi A, Bandi C, 2004. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. Int J Parasitol 34: 191203.

    • Search Google Scholar
    • Export Citation
  • 10.

    Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 15961599.

    • Search Google Scholar
    • Export Citation
  • 11.

    Saitou N, Nei M, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406425.

  • 12.

    Felsenstein J, 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evol 39: 783791.

  • 13.

    Tamura K, Nei M, Kumar S, 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101: 1103011035.

    • Search Google Scholar
    • Export Citation
  • 14.

    Post RJ, Admans Z, Shelley AJ, Maia-Herzog M, Luna Dias APA, Coscaron S, 2003. The morphological discrimination of microfilariae of Onchocerca volvulus from Mansonella ozzardi. Parasitology 127: 2127.

    • Search Google Scholar
    • Export Citation
  • 15.

    Casiraghi M, Anderson TJ, Bandi C, Bazzocchi C, Genchi C, 2001. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 122: 93103.

    • Search Google Scholar
    • Export Citation
  • 16.

    Fischer P, Büttner DW, Bamuhiiga J, Williams SA, 1998. Detection of the filarial parasite Mansonella streptocerca in skin biopsies by a nested polymerase chain reaction-based assay. Am J Trop Med Hyg 58: 816820.

    • Search Google Scholar
    • Export Citation
  • 17.

    Gasser RB, 2006. Molecular technologies in parasitology, with an emphasis on genomic approaches for investigating parasitic nematodes. Parassitologia 48: 911.

    • Search Google Scholar
    • Export Citation
  • 18.

    Manson P, 1987. On certain new species of nematode haematozoa occurring in America. Br Med J (Clin Res Ed) 2: 18371838.

  • 19.

    Shelley AJ, Luna Dias APA, Moraes MA, 1980. Simulium species of the Amazonicum group as vectors of Mansonella ozzardi in the Brazilian Amazon. Trans R Soc Trop Med Hyg 74: 784788.

    • Search Google Scholar
    • Export Citation
  • 20.

    Meditz SW, Hanratty DM, 1987. Caribbean Islands: A Country Study. Available at: http://countrystudies.us/caribbean-islands/. Accessed April 18, 2011.

    • Search Google Scholar
    • Export Citation
  • 21.

    Gonzales-Mugaburu M, 1958. Hallazgo de Mansonella ozzardi en la selva peruana. Nota preliminar. Rev Med Exp 12: 8789.

  • 22.

    Loja D, Necochea Y, Vilca M, Avilés R, 1999. Filariasis en el Perú: perfil clínico epidemiológico. Folia Dermatol Peru 10: 2730.

  • 23.

    Kozek WJ, D'Alessandro A, Silva J, Navarette S, 1982. Filariasis in Colombia: prevalence of mansonellosis in the teenage and adult population of the Colombian Bank of the Amazon, Comisaria del Amazonas. Am J Trop Med Hyg 31: 11311136.

    • Search Google Scholar
    • Export Citation
  • 24.

    Chuquicana A, Durand S, Bentley G, Sanchez JF, Flores AY, Zerpa R, Edgel KA, Huicho L, Graf PC, Andres G, 2010. Prevalence and Distribution of Filariasis by Mansonella ozzardi in the Peruvian Amazon Basin. Atlanta, GA: ASTMH.

    • Search Google Scholar
    • Export Citation
  • 25.

    Baird JK, Neafie RC, 1988. South American brugian filariasis: report of a human infection acquired in Peru. Am J Trop Med Hyg 39: 185188.

    • Search Google Scholar
    • Export Citation
  • 26.

    Beltran M, Cancrini G, Reategui G, Melgar R, Ayllon C, Garaycochea MC, Reyes R, Lammie PJ, 2008. Filariosis humana en la selva peruana: reporte de tres casos. Rev Peru Med Exp Salud Publica 25: 257260.

    • Search Google Scholar
    • Export Citation
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