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DNA sequence comparisons are useful for characterizing proposed new parasite species or strains. Microfilariae with an atypical arrangement of nuclei behind the cephalic space have been recently described in human blood samples from the Amazon region of Peru. Three blood specimens containing atypical microfilariae were genetically characterized using three DNA markers (5S ribosomal DNA, 12S ribosomal DNA, and cytochrome oxidase I). All atypical microfilariae were clustered into the Mansonella group and indistinguishable from M. ozzardi based on these DNA markers.
Financial support: This study was supported by a grant from the Barnes Jewish Hospital Foundation.
Authors' addresses: Luis A. Marcos, Gary J. Weil, and Peter U. Fischer, Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, E-mails: marcoslrz@yahoo.com, GWeil@DOM.wustl.edu, and Pufische@DOM.wustl.edu. Nancy Arrospide and Cesar Cabezas, Centro Nacional de Salud Pública, Instituto Nacional de Salud del Ministerio de Salud de Perú, Lima, Peru, E-mails: narrospide@hotmail.com and ccabezas@ins.gob.pe. Sergio Recuenco, Rabies Program, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Diseases and Prevention, Atlanta, GA, E-mail: fni9@cdc.gov.