Evaluation of In-House ELISA Using Trypanosoma cruzi Lysate and Recombinant Antigens for Diagnosis of Chagas Disease and Discrimination of Its Clinical Forms

Silvia A. Longhi Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Silvia B. Brandariz Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Sonia O. Lafon Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Leticia L. Niborski Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Alejandro O. Luquetti Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Alejandro G. Schijman Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Mariano J. Levin Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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Karina A. Gómez Laboratorio de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI), CONICET-UBA, Buenos Aires, Argentina; Instituto de Patología Tropical e Saúde Pública, Universidad Federal de Goiás, Goiania, Brasil

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The aim of this work was to investigate the potential usefulness of Trypanosoma cruzi lysate, recombinant protein JL7, and peptides P013, R13, JL18, JL19, and P0β as serological markers for human Chagas disease. We analyzed 228 sera from Brazilian Chagas disease patients classified into four clinical groups and 108 from non-chagasic patients. We defined the diagnostic sensitivity, specificity, and Kappa index measured by enzyme-linked immunosorbent assay (ELISA). As previously described, the highest values of diagnostic parameters were achieved for T. cruzi lysate and JL7; peptide P013 showed high specificity but low sensitivity. The other peptides resulted in lower sensitivity and specificity in our ELISA than T. cruzi lysate and JL7 protein. Antibodies against JL7 protein were mainly detected in sera from patients with severe chagasic cardiomyopathy, compared with those from the indeterminate form, whereas peptides failed to discriminate between the clinical forms of the disease.

Author Notes

*Address correspondence to Karina A. Gómez, Vuelta de Obligado 2490, Buenos Aires 1428. E-mails: drkagomez@gmail.com
†Deceased.

Financial support: This work was supported by grants from the World Health Organization/Special Program for Research and Training in Tropical Diseases; the Argentinean National Council for Science and Technology (CONICET), the University of Buenos Aires, and the National Agency of Scientific and Technological Promotion (FONCYT BID 1201/OC-AR, PICT 05-06802, PICT 01–14389 and BID 1728/OC-AR PICT 2439).

Authors' addresses: Silvia A. Longhi, Silvia B. Brandariz, Sonia O. Lafon, Leticia L. Niborski, and Alejandro G. Schijman, INGEBI – CONICET, Buenos Aires, E-mails: longhi@dna.uba.ar, brandariz.silvia@gmail.com, sonialafon@mincyt.gov.ar, niborski.leticia@gmail.com, and schijman@dna.uba.ar or aleschijman@gmail.com. Alejandro O. Luquetti, Instituto de Patología Tropical e Saúde Pública—Universidad Federal de Goiás, Goiania, Brazil, E-mail: aluquetti@gmail.com. Karina A. Gómez, Vuelta de Obligado 2490, Buenos Aires 1428, E-mail: drkagomez@gmail.com.

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