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Evaluation of a Rapid Point-of-Care Fecal Antigen Detection Test for Entamoeba histolytica

Poonum S. KorpeDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Blake R. StottDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Forida NazibDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Mamun KabirDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Rashidul HaqueDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Joel F. HerbeinDivision of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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William A. Petri Jr.Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia; TechLab, Inc., Blacksburg, Virginia; International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh

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Amebiasis is a major cause of morbidity and mortality in the developing world. A reliable point-of-care test would help to improve diagnosis and early treatment. We evaluated a novel rapid fecal antigen detection test for E. histolytica (E. HISTOLYTICA QUIK CHEK; TechLab, Inc., Blacksburg, VA), in a cohort of children in Bangladesh where amebiasis is endemic. This point-of-care test had a sensitivity of 100% and a specificity of 100% when compared with enzyme-linked immunosorbent assay antigen detection.

Author Notes

*Address correspondence to Poonum S. Korpe, University of Virginia Health System, Carter Harrison Building, Room 1520, 345 Crispell Drive, PO Box 801337, Charlottesville, VA 22908-1337. E-mail: psk9p@virginia.edu

Financial support: This study was supported by National Institutes of Health grant AI 43596 to William A. Petri, Jr. Dr. Petri receives licensing fees from TechLab, Inc. for amebiasis diagnostics; these fees are donated in their entirety to the American Society of Tropical Medicine and Hygiene. This study was also supported by National Institutes of Health grant 5R42AI058450 to Joel F. Herbein.

Authors' addresses: Poonum S. Korpe and William A. Petri Jr., University of Virginia Health System, Carter Harrison Building, Charlottesville, VA, E-mails: psk9p@virginia.edu and wap3g@virginia.edu. Blake R. Stott and Joel F. Herbein, TechLab, Inc., Blacksburg, VA, E-mails: bstott@techlab.com and jherbein@techlab.com. Forida Nazib, Mamun Kabir, and Rashidul Haque, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh, E-mails: forida@icddrb.org, mamunk@icddrb.org, and rhaque@icddrb.org.

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