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-
1.
The malERA Consultative Group on Diagnoses and Diagnostics, 2011. A research agenda for malaria eradication: diagnoses and diagnostics. PloS Med 8: e1000396.
-
2.
Moonen B, Cohen JM, Snow RW, Slutsker L, Drakeley C, Smith DL, Abeyasinghe RR, Rodrigues MH, Maharaj R, Tanner M, Targett G, 2010. Operational strategies to achieve and maintain malaria elimination. Lancet 376: 1592– 1603.
-
3.
Perkins M, Bell D, 2008. Working without a blindfold: the critical role of diagnostics in malaria control. Malar J 7: S5.
-
4.
Ouédraogo AL, Bousema T, Schneider P, de Vlas SJ, Ilboudo-Sanogo E, Cuzin-Ouattara N, Nébié I, Roeffen W, Berhave JP, Luty AFJ, Sauerwein R, 2009. Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. PLoS One 4: e8410.
-
5.
Steenkeste N, Rogers W, Okell L, Jeanne I, Incardona S, Duval L, Chy S, Hewitt S, Chou M, Socheat D, Babin F-X, Ariey F, Rogier C, 2010. Sub-microscopic malaria cases and mixed malaria infection in a remote area of high malaria endemicity in Rattanakiri province, Cambodia: implication for malaria elimination. Malar J 9: 108.
-
6.
Harris I, Sharrock W, Bain L, Gray K-A, Bobogare A, Boaz L, Lilley K, Krause D, Vallely A, Johnson M-L, Gatton M, Shanks G, Cheng Q, 2010. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting. Malar J 9: 254.
-
7.
Taylor SM, Juliano JJ, Trottman PA, Griffin JB, Landis SH, Kitsa P, Tshefu AK, Meshnick SR, 2010. High-throughput pooling and real-time PCR-based strategy for malaria detection. J Clin Microbiol 48: 512– 519.
-
8.
Hsiang MS, Lin M, Dokomajilar C, Kemere J, Pilcher CD, Dorsey G, Greenhouse B, 2010. PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children. J Clin Microbiol 48: 3539– 3543.
-
9.
Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52: 565– 568.
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Active Case Detection with Pooled Real-Time PCR to Eliminate Malaria in Trat Province, Thailand
Elizabeth T. Rogawski
Elizabeth T. Rogawski
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Kanungnit Congpuong
Kanungnit Congpuong
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Prayuth Sudathip
Prayuth Sudathip
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Wichai Satimai
Wichai Satimai
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Rungniran Sug-aram
Rungniran Sug-aram
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Supannee Aruncharus
Supannee Aruncharus
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Ampai Darakapong
Ampai Darakapong
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Suravadee Kitchakarn
Suravadee Kitchakarn
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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Steven R. Meshnick
Steven R. Meshnick
Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina; Bureau of Vector-Borne Diseases, Ministry of Public Health, Nonthaburi, Thailand
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We conducted contact tracing and high-risk group screening using pooled real-time polymerase chain reaction (PCR) to support malaria elimination in Thailand. PCR detected more Plasmodium infections than the local and expert microscopists. High-throughput pooling technique reduced costs and allowed prompt reporting of results.
Author Notes
Financial support: This work was funded by the Bill & Melinda Gates Foundation and the Bureau of Vector-Borne Diseases, Department of Disease Control, Ministry of Public Health, Thailand. E.T.R. was supported by a University of North Carolina Entrepreneurial Public Service Fellowship from the Carolina Center for Public Service at the University of North Carolina at Chapel Hill.
Authors' addresses: Elizabeth T. Rogawski and Steven R. Meshnick, Department of Epidemiology, University of North Carolina, Chapel Hill, NC, E-mails: rogawski@unc.edu and meshnick@email.unc.edu. Kanungnit Congpuong, Prayuth Sudathip, Wichai Satimai, Rungniran Sug-aram, Supannee Aruncharus, Ampai Darakapong, and Suravadee Kitchakarn, Bureau of Vector-Borne Disease, Department of Disease Control, Ministry of Public Health, Nonthaburi, Thailand, E-mails: nungnit@health.moph.go.th, psudathip@gmail.com, wichaisatimai@yahoo.co.th, run_rungniran@hotmail.com, imp_qmi@hotmail.com, cutetor36@yahoo.com, and kitchakarn@hotmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
The malERA Consultative Group on Diagnoses and Diagnostics, 2011. A research agenda for malaria eradication: diagnoses and diagnostics. PloS Med 8: e1000396.
-
2.
Moonen B, Cohen JM, Snow RW, Slutsker L, Drakeley C, Smith DL, Abeyasinghe RR, Rodrigues MH, Maharaj R, Tanner M, Targett G, 2010. Operational strategies to achieve and maintain malaria elimination. Lancet 376: 1592– 1603.
-
3.
Perkins M, Bell D, 2008. Working without a blindfold: the critical role of diagnostics in malaria control. Malar J 7: S5.
-
4.
Ouédraogo AL, Bousema T, Schneider P, de Vlas SJ, Ilboudo-Sanogo E, Cuzin-Ouattara N, Nébié I, Roeffen W, Berhave JP, Luty AFJ, Sauerwein R, 2009. Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. PLoS One 4: e8410.
-
5.
Steenkeste N, Rogers W, Okell L, Jeanne I, Incardona S, Duval L, Chy S, Hewitt S, Chou M, Socheat D, Babin F-X, Ariey F, Rogier C, 2010. Sub-microscopic malaria cases and mixed malaria infection in a remote area of high malaria endemicity in Rattanakiri province, Cambodia: implication for malaria elimination. Malar J 9: 108.
-
6.
Harris I, Sharrock W, Bain L, Gray K-A, Bobogare A, Boaz L, Lilley K, Krause D, Vallely A, Johnson M-L, Gatton M, Shanks G, Cheng Q, 2010. A large proportion of asymptomatic Plasmodium infections with low and sub-microscopic parasite densities in the low transmission setting of Temotu Province, Solomon Islands: challenges for malaria diagnostics in an elimination setting. Malar J 9: 254.
-
7.
Taylor SM, Juliano JJ, Trottman PA, Griffin JB, Landis SH, Kitsa P, Tshefu AK, Meshnick SR, 2010. High-throughput pooling and real-time PCR-based strategy for malaria detection. J Clin Microbiol 48: 512– 519.
-
8.
Hsiang MS, Lin M, Dokomajilar C, Kemere J, Pilcher CD, Dorsey G, Greenhouse B, 2010. PCR-based pooling of dried blood spots for detection of malaria parasites: optimization and application to a cohort of Ugandan children. J Clin Microbiol 48: 3539– 3543.
-
9.
Plowe CV, Djimde A, Bouare M, Doumbo O, Wellems TE, 1995. Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. Am J Trop Med Hyg 52: 565– 568.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 261 | 169 | 14 |
| Full Text Views | 370 | 6 | 4 |
| PDF Downloads | 85 | 3 | 0 |