ProCite
RefWorks
Reference Manager
BibTeX
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EndNote
-
1.
Takala SL, Plowe CV, 2009. Genetic diversity and malaria vaccine design, testing and efficacy: preventing and overcoming ‘vaccine resistant malaria’. Parasite Immunol 31: 560– 573.
-
2.
Escalante AA, Grebert HM, Isea R, Goldman IF, Basco L, Magris M, Biswas S, Kariuki S, Lal AA, 2002. A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas–XVI. Asembo Bay Cohort Project. Mol Biochem Parasitol 125: 83– 90.
-
3.
Egan JE, Hoffman SL, Haynes JD, Sadoff JC, Schneider I, Grau GE, Hollingdale MR, Ballou WR, Gordon DM, 1993. Humoral immune responses in volunteers immunized with irradiated Plasmodium falciparum sporozoites. Am J Trop Med Hyg 49: 166– 173.
-
4.
Nardin EH, Nussenzweig RS, McGregor IA, Bryan JH, 1979. Antibodies to sporozoites: their frequent occurrence in individuals living in an area of hyperendemic malaria. Science 206: 597– 599.
-
5.
de Groot AS, Johnson AH, Maloy WL, Quakyi IA, Riley EM, Menon A, Banks SM, Berzofsky JA, Good MF, 1989. Human T cell recognition of polymorphic epitopes from malaria circumsporozoite protein. J Immunol 142: 4000– 4005.
-
6.
Chenet SM, Branch OH, Escalante AA, Lucas CM, Bacon DJ, 2008. Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the amazon basin of peru. Malar J 7: 93.
-
7.
Weedall GD, Preston BM, Thomas AW, Sutherland CJ, Conway DJ, 2007. Differential evidence of natural selection on two leading sporozoite stage malaria vaccine candidate antigens. Int J Parasitol 37: 77– 85.
-
8.
Jalloh A, Jalloh M, Matsuoka H, 2009. T-cell epitope polymorphisms of the Plasmodium falciparum circumsporozoite protein among field isolates from sierra leone: age-dependent haplotype distribution? Malar J 8: 120.
-
9.
Bojang KA, Milligan PJ, Pinder M, Vigneron L, Alloueche A, Kester KE, Ballou WR, Conway DJ, Reece WH, Gothard P, Yamuah L, Delchambre M, Voss G, Greenwood BM, Hill A, McAdam KP, Tornieporth N, Cohen JD, Doherty TRTS, S Malaria Vaccine Trial Team, 2001. Efficacy of RTS,S/AS02 malaria vaccine against Plasmodium falciparum infection in semi-immune adult men in the Gambia: a randomised trial. Lancet 358: 1927– 1934.
-
10.
Alonso PL, Sacarlal J, Aponte JJ, Leach A, Macete E, Milman J, Mandomando I, Spiessens B, Guinovart C, Espasa M, Bassat Q, Aide P, Ofori-Anyinam O, Navia MM, Corachan S, Ceuppens M, Dubois MC, Demoitié MA, Dubovsky F, Menéndez C, Tornieporth N, Ballou WR, Thompson R, Cohen J, 2004. Efficacy of the RTS,S/AS02A vaccine against Plasmodium falciparum infection and disease in young african children: randomised controlled trial. Lancet 364: 1411– 1420.
-
11.
Aponte JJ, Aide P, Renom M, Mandomando I, Bassat Q, Sacarlal J, Manaca MN, Lafuente S, Barbosa A, Leach A, Lievens M, Vekemans J, Sigauque B, Dubois MC, Demoitié MA, Sillman M, Savarese B, McNeil JG, Macete E, Ballou WR, Cohen J, Alonso PL, 2007. Safety of the RTS,S/AS02D candidate malaria vaccine in infants living in a highly endemic area of mozambique: a double blind randomised controlled phase I/IIb trial. Lancet 370: 1543– 1551.
-
12.
Bejon P, Lusingu J, Olotu A, Leach A, Lievens M, Vekemans J, Mshamu S, Lang T, Gould J, Dubois MC, Demoitié MA, Stallaert JF, Vansadia P, Carter T, Njuguna P, Awuondo KO, Malabeja A, Abdul O, Gesase S, Mturi N, Drakeley CJ, Savarese B, Villafana T, Ballou WR, Cohen J, Riley EM, Lemnge MM, Marsh K, von Seidlein L, 2008. Efficacy of RTS,S/AS01E vaccine against malaria in children 5 to 17 months of age. N Engl J Med 359: 2521– 2532.
-
13.
Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, Methogo BG, Doucka Y, Flamen A, Mordmüller B, Issifou S, Kremsner PG, Sacarlal J, Aide P, Lanaspa M, Aponte JJ, Nhamuave A, Quelhas D, Bassat Q, Mandjate S, Macete E, Alonso P, Abdulla S, Salim N, Juma O, Shomari M, Shubis K, Machera F, Hamad AS, Minja R, Mtoro A, Sykes A, Ahmed S, Urassa AM, Ali AM, Mwangoka G, Tanner M, Tinto H, D'Alessandro U, Sorgho H, Valea I, Tahita MC, Kaboré W, Ouédraogo S, Sandrine Y, Guiguemdé RT, Ouédraogo JB, Hamel MJ, Kariuki S, Odero C, Oneko M, Otieno K, Awino N, Omoto J, Williamson J, Muturi-Kioi V, Laserson KF, Slutsker L, Otieno W, Otieno L, Nekoye O, Gondi S, Otieno A, Ogutu B, Wasuna R, Owira V, Jones D, Onyango AA, Njuguna P, Chilengi R, Akoo P, Kerubo C, Gitaka J, Maingi C, Lang T, Olotu A, Tsofa B, Bejon P, Peshu N, Marsh K, Owusu-Agyei S, Asante KP, Osei-Kwakye K, Boahen O, Ayamba S, Kayan K, Owusu-Ofori R, Dosoo D, Asante I, Adjei G, Adjei G, Chandramohan D, Greenwood B, Lusingu J, Gesase S, Malabeja A, Abdul O, Kilavo H, Mahende C, Liheluka E, Lemnge M, Theander T, Drakeley C, Ansong D, Agbenyega T, Adjei S, Boateng HO, Rettig T, Bawa J, Sylverken J, Sambian D, Agyekum A, Owusu L, Martinson F, Hoffman I, Mvalo T, Kamthunzi P, Nkomo R, Msika A, Jumbe A, Chome N, Nyakuipa D, Chintedza J, Ballou WR, Bruls M, Cohen J, Guerra Y, Jongert E, Lapierre D, Leach A, Lievens M, Ofori-Anyinam O, Vekemans J, Carter T, Leboulleux D, Loucq C, Radford A, Savarese B, Schellenberg D, Sillman M, Vansadia PRTS,S Clinical Trials Partnership, 2011. First results of phase 3 trial of RTS,S/AS01 malaria vaccine in African children. N Engl J Med 365: 1863– 1875.
-
14.
Alloueche A, Milligan P, Conway DJ, Pinder M, Bojang K, Doherty T, Tornieporth N, Cohen J, Greenwood BM, 2003. Protective efficacy of the RTS,S/AS02 Plasmodium falciparum malaria vaccine is not strain specific. Am J Trop Med Hyg 68: 97– 101.
-
15.
Carr IM, Robinson JI, Dimitriou R, Markham AF, Morgan AW, Bonthron DT, 2009. Inferring relative proportions of DNA variants from sequencing electropherograms. Bioinformatics 25: 3244– 3250.
-
16.
Coulibaly D, Diallo DA, Thera MA, Dicko A, Guindo AB, Koné AK, Cissoko Y, Coulibaly S, Djimdé A, Lyke K, Doumbo OK, Plowe CV, 2002. Impact of preseason treatment on incidence of falciparum malaria and parasite density at a site for testing malaria vaccines in Bandiagara, Mali. Am J Trop Med Hyg 67: 604– 610.
-
17.
Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD, 2006. Amplification of complex gene libraries by emulsion PCR. Nat Methods 3: 545– 550.
-
18.
Hunt P, Fawcett R, Carter R, Walliker D, 2005. Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications. Mol Biochem Parasitol 143: 173– 182.
-
19.
Ochola LI, Tetteh KK, Stewart LB, Riitho V, Marsh K, Conway DJ, 2010. Allele frequency-based and polymorphism-versus-divergence indices of balancing selection in a new filtered set of polymorphic genes in Plasmodium falciparum. Mol Biol Evol 27: 2344– 2351.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 976 | 843 | 17 |
| Full Text Views | 373 | 9 | 0 |
| PDF Downloads | 83 | 8 | 0 |
Next Generation Sequencing to Detect Variation in the Plasmodium falciparum Circumsporozoite Protein
Kavita Gandhi
Kavita Gandhi
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Mahamadou A. Thera
Mahamadou A. Thera
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Drissa Coulibaly
Drissa Coulibaly
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Karim Traoré
Karim Traoré
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Ando B. Guindo
Ando B. Guindo
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Ogobara K. Doumbo
Ogobara K. Doumbo
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Shannon Takala-Harrison
Shannon Takala-Harrison
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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Christopher V. Plowe
Christopher V. Plowe
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Malaria Research and Training Center, University of Bamako, Bamako, Mali; Howard Hughes Medical Institute, University of Maryland School of Medicine, Baltimore, Maryland
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The malaria vaccine RTS,S/AS01, based on immunogenic regions of the Plasmodium falciparum circumsporozoite protein (CSP), has partial efficacy against clinical malaria in African children. Understanding how sequence diversity in CSP T- and B-cell epitopes relates to naturally acquired and vaccine-induced immunity may be useful in efforts to improve the efficacy of CSP-based vaccines. However, limitations in sequencing technology have precluded thorough evaluation of diversity in the immunogenic regions of this protein. In this study, 454, a next generation sequencing technology, was evaluated as a method for assessing diversity in these regions. Portions of the circumsporozoite gene (cs) were sequenced both by 454 and Sanger sequencing from samples collected in a study in Bandiagara, Mali. 454 detected more single nucleotide polymorphisms and haplotypes in the T-cell epitopes than Sanger sequencing, and it was better able to resolve genetic diversity in samples with multiple infections; however, it failed to generate sequence for the B-cell epitopes.
Author Notes
Financial support: This research was supported by Contract N01AI85346 and Cooperative Agreement U19AI065683 from the National Institute of Allergy and Infectious Diseases (NIAID), Grant D43TW001589 from the Fogarty International Center, National Institutes of Health, and Contract W81XWH-06-1-0427 from the US Department of Defense and the US Agency for International Development. S.T.-H. is supported by the University of Maryland Multidisciplinary Clinical Research Career Development Program (National Institutes of Health Grant K12RR023250). C.V.P. is supported by a Distinguished Clinical Scientist Award from the Doris Duke Charitable Foundation and by the Howard Hughes Medical Institute.
Authors' addresses: Kavita Gandhi, Shannon Takala-Harrison, and Christopher V. Plowe, Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland, Baltimore, MD, E-mails: Kavita.Gandhi@som.umaryland.edu, stakala@medicine.umaryland.edu, and cplowe@medicine.umaryland.edu. Mahamadou A. Thera, Drissa Coulibaly, Karim Traoré, Ando B. Guindo, and Ogobara K. Doumbo, Malaria Research and Training Center, University of Bamako, Bamako, Mali. E-mails: mthera@icermali.org, coulibalyd@icermali.org, karim@icermali.org, ando@icermali.org, and okd@icermali.org.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Takala SL, Plowe CV, 2009. Genetic diversity and malaria vaccine design, testing and efficacy: preventing and overcoming ‘vaccine resistant malaria’. Parasite Immunol 31: 560– 573.
-
2.
Escalante AA, Grebert HM, Isea R, Goldman IF, Basco L, Magris M, Biswas S, Kariuki S, Lal AA, 2002. A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas–XVI. Asembo Bay Cohort Project. Mol Biochem Parasitol 125: 83– 90.
-
3.
Egan JE, Hoffman SL, Haynes JD, Sadoff JC, Schneider I, Grau GE, Hollingdale MR, Ballou WR, Gordon DM, 1993. Humoral immune responses in volunteers immunized with irradiated Plasmodium falciparum sporozoites. Am J Trop Med Hyg 49: 166– 173.
-
4.
Nardin EH, Nussenzweig RS, McGregor IA, Bryan JH, 1979. Antibodies to sporozoites: their frequent occurrence in individuals living in an area of hyperendemic malaria. Science 206: 597– 599.
-
5.
de Groot AS, Johnson AH, Maloy WL, Quakyi IA, Riley EM, Menon A, Banks SM, Berzofsky JA, Good MF, 1989. Human T cell recognition of polymorphic epitopes from malaria circumsporozoite protein. J Immunol 142: 4000– 4005.
-
6.
Chenet SM, Branch OH, Escalante AA, Lucas CM, Bacon DJ, 2008. Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the amazon basin of peru. Malar J 7: 93.
-
7.
Weedall GD, Preston BM, Thomas AW, Sutherland CJ, Conway DJ, 2007. Differential evidence of natural selection on two leading sporozoite stage malaria vaccine candidate antigens. Int J Parasitol 37: 77– 85.
-
8.
Jalloh A, Jalloh M, Matsuoka H, 2009. T-cell epitope polymorphisms of the Plasmodium falciparum circumsporozoite protein among field isolates from sierra leone: age-dependent haplotype distribution? Malar J 8: 120.
-
9.
Bojang KA, Milligan PJ, Pinder M, Vigneron L, Alloueche A, Kester KE, Ballou WR, Conway DJ, Reece WH, Gothard P, Yamuah L, Delchambre M, Voss G, Greenwood BM, Hill A, McAdam KP, Tornieporth N, Cohen JD, Doherty TRTS, S Malaria Vaccine Trial Team, 2001. Efficacy of RTS,S/AS02 malaria vaccine against Plasmodium falciparum infection in semi-immune adult men in the Gambia: a randomised trial. Lancet 358: 1927– 1934.
-
10.
Alonso PL, Sacarlal J, Aponte JJ, Leach A, Macete E, Milman J, Mandomando I, Spiessens B, Guinovart C, Espasa M, Bassat Q, Aide P, Ofori-Anyinam O, Navia MM, Corachan S, Ceuppens M, Dubois MC, Demoitié MA, Dubovsky F, Menéndez C, Tornieporth N, Ballou WR, Thompson R, Cohen J, 2004. Efficacy of the RTS,S/AS02A vaccine against Plasmodium falciparum infection and disease in young african children: randomised controlled trial. Lancet 364: 1411– 1420.
-
11.
Aponte JJ, Aide P, Renom M, Mandomando I, Bassat Q, Sacarlal J, Manaca MN, Lafuente S, Barbosa A, Leach A, Lievens M, Vekemans J, Sigauque B, Dubois MC, Demoitié MA, Sillman M, Savarese B, McNeil JG, Macete E, Ballou WR, Cohen J, Alonso PL, 2007. Safety of the RTS,S/AS02D candidate malaria vaccine in infants living in a highly endemic area of mozambique: a double blind randomised controlled phase I/IIb trial. Lancet 370: 1543– 1551.
-
12.
Bejon P, Lusingu J, Olotu A, Leach A, Lievens M, Vekemans J, Mshamu S, Lang T, Gould J, Dubois MC, Demoitié MA, Stallaert JF, Vansadia P, Carter T, Njuguna P, Awuondo KO, Malabeja A, Abdul O, Gesase S, Mturi N, Drakeley CJ, Savarese B, Villafana T, Ballou WR, Cohen J, Riley EM, Lemnge MM, Marsh K, von Seidlein L, 2008. Efficacy of RTS,S/AS01E vaccine against malaria in children 5 to 17 months of age. N Engl J Med 359: 2521– 2532.
-
13.
Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, Methogo BG, Doucka Y, Flamen A, Mordmüller B, Issifou S, Kremsner PG, Sacarlal J, Aide P, Lanaspa M, Aponte JJ, Nhamuave A, Quelhas D, Bassat Q, Mandjate S, Macete E, Alonso P, Abdulla S, Salim N, Juma O, Shomari M, Shubis K, Machera F, Hamad AS, Minja R, Mtoro A, Sykes A, Ahmed S, Urassa AM, Ali AM, Mwangoka G, Tanner M, Tinto H, D'Alessandro U, Sorgho H, Valea I, Tahita MC, Kaboré W, Ouédraogo S, Sandrine Y, Guiguemdé RT, Ouédraogo JB, Hamel MJ, Kariuki S, Odero C, Oneko M, Otieno K, Awino N, Omoto J, Williamson J, Muturi-Kioi V, Laserson KF, Slutsker L, Otieno W, Otieno L, Nekoye O, Gondi S, Otieno A, Ogutu B, Wasuna R, Owira V, Jones D, Onyango AA, Njuguna P, Chilengi R, Akoo P, Kerubo C, Gitaka J, Maingi C, Lang T, Olotu A, Tsofa B, Bejon P, Peshu N, Marsh K, Owusu-Agyei S, Asante KP, Osei-Kwakye K, Boahen O, Ayamba S, Kayan K, Owusu-Ofori R, Dosoo D, Asante I, Adjei G, Adjei G, Chandramohan D, Greenwood B, Lusingu J, Gesase S, Malabeja A, Abdul O, Kilavo H, Mahende C, Liheluka E, Lemnge M, Theander T, Drakeley C, Ansong D, Agbenyega T, Adjei S, Boateng HO, Rettig T, Bawa J, Sylverken J, Sambian D, Agyekum A, Owusu L, Martinson F, Hoffman I, Mvalo T, Kamthunzi P, Nkomo R, Msika A, Jumbe A, Chome N, Nyakuipa D, Chintedza J, Ballou WR, Bruls M, Cohen J, Guerra Y, Jongert E, Lapierre D, Leach A, Lievens M, Ofori-Anyinam O, Vekemans J, Carter T, Leboulleux D, Loucq C, Radford A, Savarese B, Schellenberg D, Sillman M, Vansadia PRTS,S Clinical Trials Partnership, 2011. First results of phase 3 trial of RTS,S/AS01 malaria vaccine in African children. N Engl J Med 365: 1863– 1875.
-
14.
Alloueche A, Milligan P, Conway DJ, Pinder M, Bojang K, Doherty T, Tornieporth N, Cohen J, Greenwood BM, 2003. Protective efficacy of the RTS,S/AS02 Plasmodium falciparum malaria vaccine is not strain specific. Am J Trop Med Hyg 68: 97– 101.
-
15.
Carr IM, Robinson JI, Dimitriou R, Markham AF, Morgan AW, Bonthron DT, 2009. Inferring relative proportions of DNA variants from sequencing electropherograms. Bioinformatics 25: 3244– 3250.
-
16.
Coulibaly D, Diallo DA, Thera MA, Dicko A, Guindo AB, Koné AK, Cissoko Y, Coulibaly S, Djimdé A, Lyke K, Doumbo OK, Plowe CV, 2002. Impact of preseason treatment on incidence of falciparum malaria and parasite density at a site for testing malaria vaccines in Bandiagara, Mali. Am J Trop Med Hyg 67: 604– 610.
-
17.
Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD, 2006. Amplification of complex gene libraries by emulsion PCR. Nat Methods 3: 545– 550.
-
18.
Hunt P, Fawcett R, Carter R, Walliker D, 2005. Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications. Mol Biochem Parasitol 143: 173– 182.
-
19.
Ochola LI, Tetteh KK, Stewart LB, Riitho V, Marsh K, Conway DJ, 2010. Allele frequency-based and polymorphism-versus-divergence indices of balancing selection in a new filtered set of polymorphic genes in Plasmodium falciparum. Mol Biol Evol 27: 2344– 2351.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 976 | 843 | 17 |
| Full Text Views | 373 | 9 | 0 |
| PDF Downloads | 83 | 8 | 0 |