Identification of Oropouche Orthobunyavirus in the Cerebrospinal Fluid of Three Patients in the Amazonas, Brazil

Michele de Souza Bastos Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Luiz Tadeu Moraes Figueiredo Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Felipe Gomes Naveca Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Rossicleia Lins Monte Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Natália Lessa Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Regina Maria Pinto de Figueiredo Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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João Bosco de Lima Gimaque Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Guilherme Pivoto João Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Rajendranath Ramasawmy Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Maria Paula Gomes Mourão Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil

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Oropouche fever is the second most frequent arboviral infection in Brazil, surpassed only by dengue. Oropouche virus (OROV) causes large and explosive outbreaks of acute febrile illness in cities and villages in the Amazon and Central-Plateau regions. Cerebrospinal fluid (CSF) samples from 110 meningoencephalitis patients were analyzed. The RNA extracted from fluid was submitted to reverse transcription-polymerase chain reaction and sequencing to identify OROV. Three CSF samples showed the presence of OROV causing infection in the central nervous system (CNS). These patients are adults. Two of the patients had other diseases affecting CNS and immune systems: neurocysticercosis and acquired immunodeficiency syndrome, respectively. Nucleotide sequence analysis showed that the OROV from the CSF of these patients belonged to genotype I. We show here that severe Oropouche disease is occurring during outbreaks of this virus in Brazil

Author Notes

*Address correspondence to Michele de Souza Bastos, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Av. Pedro Teixeira, 25 – Dom Pedro Manaus-Am, Brazil, CEP: 69040-000. E-mails: Michele@fmt.am.gov.br or Michelebastos01@gmail.com

Financial support: This research was supported by the Amazonas Research Supporting Foundation (FAPEAM grant 871/10) to Michele de Souza Bastos and (FAPEAM grant 887/10) to Maria Paula Gomes Mourão.

Authors' addresses: Michele de Souza Bastos and João Bosco de Lima Gimaque, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University of State of Amazonas, Manaus, AM, Brazil, E-mails: Michele@fmt.am.gov.br and gimaque@ig.com.br. Rossicleia Lins Monte, Regina Maria Pinto de Figueiredo, and Guilherme Pivoto João, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil, E-mails: rossi@fmt.am.gov.br, figueiredormp@yahoo.com.br, and guilhermepivoto@hotmail.com. Rajendranath Ramasawmy, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; Nilton Lins University Center, Manaus, AM, Brasil, E-mail: ramasawm@gmail.com. Luiz Tadeu Moraes Figueiredo, School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil, E-mail: ltmfigue@fmrp.usp.br. Felipe Gomes Naveca, Instituto Leônidas e Maria Deane - FIOCRUZ, Amazônia, Manaus, Brasil, E-mail: fnaveca@amazonia.fiocruz.br. Natália Lessa, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil, E-mail: naty_lessa@hotmail.com. Maria Paula Gomes Mourão, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University of State of Amazonas, Manaus, AM, Brazil; Nilton Lins University Center, Manaus, AM, Brasil, E-mail: mariapaula.mourao@gmail.com.

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