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1.
Elliot RM, Bouloy M, Calisher CH, Goldbach R, Moyer JT, Nichol ST, Pettersson R, Plyusnin A, Schmaljohn CS, 2000. Bunyaviridae. Van Regenmortel MH, Fauquet CM, Bishop DH, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB, eds. Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Academic Press, 599– 621.
-
2.
Pinheiro FP, Hoch AL, Gomes ML, Roberts DR, 1981. Oropouche virus. IV. Laboratory transmission by Culicoides paraensis. Am J Trop Med Hyg 30: 172– 176.
-
3.
Bernardes-Terzian AC, de Moraes-Bronzoni RV, Drumond BP, Da Silva-Nunes M, da-Silva NS, Urbano-Ferreira M, Sperança MA, Nogueira ML, 2009. Sporadic oropouche virus infection, acre, Brazil. Emerg Infect Dis 15: 348– 350.
-
4.
Mourão MP, Bastos MS, Gimaque JB, Mota BR, Souza GS, Grimmer GH, Galusso ES, Arruda E, Figueiredo LT, 2009. Oropouche fever outbreak, Manaus, Brazil, 2007–2008. Emerg Infect Dis 15: 2063– 2064.
-
5.
Pinheiro FP, Rocha AG, Freitas RB, Ohana BA, Travassos da Rosa AP, Rogério JS, Linhares AC, 1982. Meningitis associated with Oropouche virus infections. Rev Inst Med Trop Sao Paulo 24: 246– 251.
-
6.
Mendoza LP, Bronzoni RV, Takayanagui OM, Aquino VH, Figueiredo LT, 2007. Viral infections of the central nervous system in Brazil. J Infect 54: 589– 596.
-
7.
Shope RE, Woodall JP, Travassos da Rosa AP, 1998. The epidemiology of diseases caused by viruses in Groups C and Guama (Bunyaviridae). Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Fifth edition. Boca Raton, FL: CRC Press, 37– 52.
-
8.
Moreli ML, Aquino VH, Cruz AC, Figueiredo LT, 2002. Diagnosis of Oropouche virus infection by RT-nested-PCR. J Med Virol 66: 139– 142.
-
9.
Markoulatos P, Georgopoulou A, Siafakas N, Plakokefalos E, Tzanakaki G, Kourea-Kremastinou J, 2001. Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay. J Clin Microbiol 39: 4426– 4432.
-
10.
Dos Santos GP, Skraba I, Oliveira D, Lima AA, de Melo MM, Kmetzsch CI, da Costa EV, da Silva EE, 2006. Enterovirus meningitis in Brazil, 1998–2003. J Med Virol 78: 98– 104.
-
11.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1990. Basic local alignment search tool. J Mol Biol 215: 403– 410.
-
12.
Figueiredo LT, Shope RE, 1987. An enzyme immunoassay for dengue antibody using infected cultured mosquito cells as antigen. J Virol Methods 17: 191– 198.
-
13.
Saeed MF, Wang H, Nunes M, Vasconcelos PF, Weaver SC, Shope RE, Watts DM, Tesh RB, Barrett AD, 2000. Nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche virus. J Gen Virol 81: 743– 748.
-
14.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG, 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947– 2948.
-
15.
Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596– 1599.
-
16.
Vasconcelos HB, Azevedo RS, Casseb SM, Nunes-Neto JP, Chiang JO, Cantuária PC, Segura MN, Martins LC, Monteiro HA, Rodrigues SG, Nunes MR, Vasconcelos PF, 2009. Oropouche fever epidemic in northern Brazil: epidemiology and molecular characterization of isolates. J Clin Virol 44: 129– 133.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2481 | 2255 | 399 |
| Full Text Views | 1481 | 76 | 18 |
| PDF Downloads | 244 | 85 | 20 |
Identification of Oropouche Orthobunyavirus in the Cerebrospinal Fluid of Three Patients in the Amazonas, Brazil
Michele de Souza Bastos
Michele de Souza Bastos
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Luiz Tadeu Moraes Figueiredo
Luiz Tadeu Moraes Figueiredo
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Felipe Gomes Naveca
Felipe Gomes Naveca
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Rossicleia Lins Monte
Rossicleia Lins Monte
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Natália Lessa
Natália Lessa
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Regina Maria Pinto de Figueiredo
Regina Maria Pinto de Figueiredo
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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João Bosco de Lima Gimaque
João Bosco de Lima Gimaque
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Guilherme Pivoto João
Guilherme Pivoto João
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Rajendranath Ramasawmy
Rajendranath Ramasawmy
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Maria Paula Gomes Mourão
Maria Paula Gomes Mourão
Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, University of State of Amazonas, Manaus, AM, Brazil; School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil; Instituto Le?nidas e Maria Deane - FIOCRUZ, Amaz?nia, Manaus, Brasil; Nilton Lins University Center, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil
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Oropouche fever is the second most frequent arboviral infection in Brazil, surpassed only by dengue. Oropouche virus (OROV) causes large and explosive outbreaks of acute febrile illness in cities and villages in the Amazon and Central-Plateau regions. Cerebrospinal fluid (CSF) samples from 110 meningoencephalitis patients were analyzed. The RNA extracted from fluid was submitted to reverse transcription-polymerase chain reaction and sequencing to identify OROV. Three CSF samples showed the presence of OROV causing infection in the central nervous system (CNS). These patients are adults. Two of the patients had other diseases affecting CNS and immune systems: neurocysticercosis and acquired immunodeficiency syndrome, respectively. Nucleotide sequence analysis showed that the OROV from the CSF of these patients belonged to genotype I. We show here that severe Oropouche disease is occurring during outbreaks of this virus in Brazil
Author Notes
Financial support: This research was supported by the Amazonas Research Supporting Foundation (FAPEAM grant 871/10) to Michele de Souza Bastos and (FAPEAM grant 887/10) to Maria Paula Gomes Mourão.
Authors' addresses: Michele de Souza Bastos and João Bosco de Lima Gimaque, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University of State of Amazonas, Manaus, AM, Brazil, E-mails: Michele@fmt.am.gov.br and gimaque@ig.com.br. Rossicleia Lins Monte, Regina Maria Pinto de Figueiredo, and Guilherme Pivoto João, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil, E-mails: rossi@fmt.am.gov.br, figueiredormp@yahoo.com.br, and guilhermepivoto@hotmail.com. Rajendranath Ramasawmy, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; Nilton Lins University Center, Manaus, AM, Brasil, E-mail: ramasawm@gmail.com. Luiz Tadeu Moraes Figueiredo, School of Medicine of the University of São Paulo in Ribeirão Preto, SP, Brazil, E-mail: ltmfigue@fmrp.usp.br. Felipe Gomes Naveca, Instituto Leônidas e Maria Deane - FIOCRUZ, Amazônia, Manaus, Brasil, E-mail: fnaveca@amazonia.fiocruz.br. Natália Lessa, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University Federal of Amazon, Manaus, AM, Brazil, E-mail: naty_lessa@hotmail.com. Maria Paula Gomes Mourão, Fundação De Medicina Tropical – Dr. Heitor Vieira Dourado, Manaus, AM, Brasil; University of State of Amazonas, Manaus, AM, Brazil; Nilton Lins University Center, Manaus, AM, Brasil, E-mail: mariapaula.mourao@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Elliot RM, Bouloy M, Calisher CH, Goldbach R, Moyer JT, Nichol ST, Pettersson R, Plyusnin A, Schmaljohn CS, 2000. Bunyaviridae. Van Regenmortel MH, Fauquet CM, Bishop DH, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB, eds. Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Academic Press, 599– 621.
-
2.
Pinheiro FP, Hoch AL, Gomes ML, Roberts DR, 1981. Oropouche virus. IV. Laboratory transmission by Culicoides paraensis. Am J Trop Med Hyg 30: 172– 176.
-
3.
Bernardes-Terzian AC, de Moraes-Bronzoni RV, Drumond BP, Da Silva-Nunes M, da-Silva NS, Urbano-Ferreira M, Sperança MA, Nogueira ML, 2009. Sporadic oropouche virus infection, acre, Brazil. Emerg Infect Dis 15: 348– 350.
-
4.
Mourão MP, Bastos MS, Gimaque JB, Mota BR, Souza GS, Grimmer GH, Galusso ES, Arruda E, Figueiredo LT, 2009. Oropouche fever outbreak, Manaus, Brazil, 2007–2008. Emerg Infect Dis 15: 2063– 2064.
-
5.
Pinheiro FP, Rocha AG, Freitas RB, Ohana BA, Travassos da Rosa AP, Rogério JS, Linhares AC, 1982. Meningitis associated with Oropouche virus infections. Rev Inst Med Trop Sao Paulo 24: 246– 251.
-
6.
Mendoza LP, Bronzoni RV, Takayanagui OM, Aquino VH, Figueiredo LT, 2007. Viral infections of the central nervous system in Brazil. J Infect 54: 589– 596.
-
7.
Shope RE, Woodall JP, Travassos da Rosa AP, 1998. The epidemiology of diseases caused by viruses in Groups C and Guama (Bunyaviridae). Monath TP, ed. The Arboviruses: Epidemiology and Ecology. Fifth edition. Boca Raton, FL: CRC Press, 37– 52.
-
8.
Moreli ML, Aquino VH, Cruz AC, Figueiredo LT, 2002. Diagnosis of Oropouche virus infection by RT-nested-PCR. J Med Virol 66: 139– 142.
-
9.
Markoulatos P, Georgopoulou A, Siafakas N, Plakokefalos E, Tzanakaki G, Kourea-Kremastinou J, 2001. Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay. J Clin Microbiol 39: 4426– 4432.
-
10.
Dos Santos GP, Skraba I, Oliveira D, Lima AA, de Melo MM, Kmetzsch CI, da Costa EV, da Silva EE, 2006. Enterovirus meningitis in Brazil, 1998–2003. J Med Virol 78: 98– 104.
-
11.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1990. Basic local alignment search tool. J Mol Biol 215: 403– 410.
-
12.
Figueiredo LT, Shope RE, 1987. An enzyme immunoassay for dengue antibody using infected cultured mosquito cells as antigen. J Virol Methods 17: 191– 198.
-
13.
Saeed MF, Wang H, Nunes M, Vasconcelos PF, Weaver SC, Shope RE, Watts DM, Tesh RB, Barrett AD, 2000. Nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche virus. J Gen Virol 81: 743– 748.
-
14.
Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG, 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947– 2948.
-
15.
Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596– 1599.
-
16.
Vasconcelos HB, Azevedo RS, Casseb SM, Nunes-Neto JP, Chiang JO, Cantuária PC, Segura MN, Martins LC, Monteiro HA, Rodrigues SG, Nunes MR, Vasconcelos PF, 2009. Oropouche fever epidemic in northern Brazil: epidemiology and molecular characterization of isolates. J Clin Virol 44: 129– 133.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2481 | 2255 | 399 |
| Full Text Views | 1481 | 76 | 18 |
| PDF Downloads | 244 | 85 | 20 |