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Evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Malaria Diagnosis in a Field Setting

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  • Vector Borne Disease Training Center, Pra Budhabat, Saraburi, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Ehime, Japan; Department of Parasitology, Kangwon National University College of Medicine, Chunchon, South Korea; Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; Venture Business Laboratory, Ehime University, Matsuyama, Ehime, Japan; Mahidol Vivax Research Center, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.

Author Notes

*Address correspondence to Sureemas Buates, Department of Microbiology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. E-mail: sbuates@hotmail.com

Financial support: This study was supported by the World Health Organization/Southeast Asia Regional Office (OSER2 P2 A4, AMS Code: 6149175).

Authors' addresses: Jeeraphat Sirichaisinthop, Vector Borne Disease Training Center, Pra Budhabat, Saraburi 18120, Thailand, E-mail: grphat@yahoo.com. Sureemas Buates, Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand, E-mail: sbuates@hotmail.com. Risa Watanabe and Satoru Takeo, Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Ehime 790-8577, Japan, E-mails: risa@m.ehime-u.ac.jp and satoru@m.ehime-u.ac.jp. Eun-Taek Han, Department of Parasitology, Kangwon National University College of Medicine, Chunchon 200-701, South Korea, E-mail: etaekhan@yahoo.com. Wachira Suktawonjaroenpon and Somporn Krasaesub, Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok 10400, Thailand, E-mails: wachiras@afrims.org and sompornk@afrims.org. Takafumi Tsuboi, Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University, Matsuyama, Ehime 790-8577, Japan, E-mail: tsuboi@ccr.ehime-u.ac.jp. Jetsumon Sattabongkot, Mahidol Vivax Research Center, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, E-mails: tmjetsumon@mahidol.ac.th and jetsumon@hotmail.com.

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