Author:
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Ricaldi JN, Vinetz JM, 2006. Leptospirosis in the tropics and in travelers. Curr Infect Dis Rep 8: 51–58.
-
2.
Ko AI, Goarant C, Picardeau M, 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7: 736–747.
-
3.
Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757–771.
-
4.
Gouveia EL, Metcalfe J, de Carvalho AL, Aires TS, Villasboas-Bisneto JC, Queirroz A, Santos AC, Salgado K, Reis MG, Ko AI, 2008. Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. Emerg Infect Dis 14: 505–508.
-
5.
Arean VM, 1962. Studies on the pathogenesis of leptospirosis. II. A clinicopathologic evaluation of hepatic and renal function in experimental leptospiral infections. Lab Invest 11: 273–288.
-
6.
Silva ÉF, Santos CS, Athanazio DA, Seyffert N, Seixas FK, Cerqueira GM, Fagundes MQ, Brod CS, Reis MG, Dellagostin OA, Ko AI, 2008. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine 26: 3892–3896.
-
7.
Sanger VL, Hamdy AH, Fizette WB, Bohl EH, Ferguson LC, 1961. Leptospira pomona infection in hamsters. Cornell Vet 51: 489–498.
-
8.
Badiola J, Thiermann AB, Cheville NF, 1983. Pathologic features of leptospirosis in hamsters caused by Leptospira interrogans serovars hardjo and szwajizak. Am J Vet Res 44: 91–99.
-
9.
Barnett JK, Barnett D, Bolin CA, Summers TA, Wagar EA, Cheville NF, Hartskeerl RA, Haake DA, 1999. Expression and distribution of leptospiral outer membrane components during renal infection of hamsters. Infect Immun 67: 853–861.
-
10.
Slack AT, Symonds ML, Dohnt MF, Craig SB, Smythe LD, 2010. Molecular Epidemiology of Leptospira borgpetersenii serovar Arborea, Queensland, Australia, 1998–2005. Am J Trop Med Hyg 83: 820–821.
-
11.
Herrmann Storck C, Postic D, Lamaury I, Perez JM, 2008. Changes in epidemiology of leptospirosis in 2003–2004, a two El Niño Southern Oscillation period, Guadeloupe archipelago, French West Indies. Epidemiol Infect 136: 1407–1415.
-
12.
Thornley CN, Baker MG, Weinstein P, Maas EW, 2002. Changing epidemiology of human leptospirosis in New Zealand. Epidemiol Infect 128: 29–36.
-
13.
Vieira ML, Gama-Simões MJ, Collares-Pereira M, 2006. Human leptospirosis in Portugal: a retrospective study of eighteen years. Int J Infect Dis 10: 378–386.
-
14.
Silva ÉF, Felix SR, Cerqueira GM, Fagundes MQ, Seixas Neto ACP, Grassmann AA, Amaral MG, Gallina T, Dellagostin OA, 2010. Preliminary characterization of Mus musculus-derived pathogenic strains of Leptospira borgpetersenii serogroup Ballum in a hamster model. Am J Trop Med Hyg 83: 336–337.
-
15.
Faine SB, Adler B, Bolin C, Perolat P, 1999. Leptospira and Leptospirosis. Melbourne, Australia: MediSci.
-
16.
Reed LJ, Muench H, 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493–497.
-
17.
Sonrier C, Branger C, Michel V, Ruvoën-Clouet N, Ganière JP, André-Fontaine G, 2000. Evidence of cross-protection within Leptospira interrogans in an experimental model. Vaccine 19: 86–94.
-
18.
Wang Z, Jin L, Wegrzyn A, 2007. Leptospirosis vaccines. Microb Cell Fact 6: 39–49.
-
19.
Palaniappan RUM, McDonough SP, Divers TJ, Chen CS, Pan MJ, Matsumoto M, Chang YF, 2006. Immunoprotection of recombinant Leptospiral immunoglobulin-like protein a against Leptospira interrogans serovar pomona infection. Infect Immun 74: 1745–1750.
-
20.
Chang YF, Chen CS, Palaniappan RUM, He H, McDonough SP, Barr SC, Yan W, Faisal SM, Pan MJ, Chang CF, 2007. Immunogenicity of the recombinant leptospiral putative outer membrane proteins as vaccine candidates. Vaccine 25: 8190–8197.
-
21.
Silva ÉF, Medeiros MA, McBride AJA, Matsunaga J, Esteves GS, Ramos JGR, Santos CS, Croda J, Homma A, Dellagostin OA, Haake DA, Reis MG, Ko AI, 2007. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine 25: 6277–6286.
-
22.
Faisal SM, Yan W, Chen C, Palaniappan RUM, McDonough SP, Chang YF, 2008. Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters. Vaccine 26: 277–287.
-
23.
Suepaul SM, Carrington CV, Campbell M, Borde G, Adesiyun AA, 2010. Study on the efficacy of Leptospira vaccines developed from serovars isolated from Trinidad and comparison with commercial vaccines using a hamster model. Vaccine 28: 5421–5426.
-
24.
Felix SR, Silva ÉF, Jouglard SDD, Hartmann DM, Grassmann AA, Dellagostin OA, 2009. Leptospirosis vaccine: search for subunit candidates. Proc Vaccinol 1: 110–114.
-
25.
Santos CS, Macedo JO, Bandeira M, Chagas-Junior AD, McBride AJ, McBride FW, Reis MG, Athanazio DA, 2010. Different outcomes of experimental leptospiral infection in mouse strains with distinct genotypes. J Med Microbiol 59: 1101–1106.
-
26.
Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, Davis J, Johnson M, Kuczek E, Alt DP, Peterson-Burch B, Coppel RL, Rood JI, Davies JK, Adler B, 2006. Genome reduction in Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci USA 103: 14560–14565.
-
27.
Nascimento ALTO, Ko AI, Martins EAL, Monteiro-Vitorello CB, Ho PL, Haake DA, 2004. Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186: 2164–2172.
-
28.
Adler B, Moctezuma AD, 2010. Leptospira and leptospirosis. Vet Microbiol 140: 287–296.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 22 | 22 | 6 |
| Full Text Views | 273 | 64 | 1 |
| PDF Downloads | 52 | 14 | 1 |
Highly Virulent Leptospira borgpetersenii Strain Characterized in the Hamster Model
Juliana Alcoforado Diniz
Juliana Alcoforado Diniz
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Samuel Rodrigues Félix
Samuel Rodrigues Félix
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Josiane Bonel-Raposo
Josiane Bonel-Raposo
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Amilton Clair Pinto Seixas Neto
Amilton Clair Pinto Seixas Neto
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Flávia Aleixo Vasconcellos
Flávia Aleixo Vasconcellos
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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André Alex Grassmann
André Alex Grassmann
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Odir Antônio Dellagostin
Odir Antônio Dellagostin
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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José Antonio Guimarães Aleixo
José Antonio Guimarães Aleixo
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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Éverton Fagonde da Silva
Éverton Fagonde da Silva
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil; Faculdade de Veterinária and Programa de Pós-Graduação em Veterinária (UFPel), Universidade Federal de Pelotas (CDTec-UFPel), Pelotas, RS, Brazil
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A recent study by our group reported the isolation and partial serological and molecular characterization of four Leptospira borgpetersenii serogroup Ballum strains. Here, we reproduced experimental leptospirosis in golden Syrian hamsters (Mesocricetus auratus) and carried out standardization of lethal dose 50% (LD50) of one of these strains (4E). Clinical disease features and histopathologic analyses of tissue lesions were also observed. As results, strain 4E induced lethality in the hamster model with inocula lower than 10 leptospires, and histopathological examination of animals showed typical lesions found in severe leptospirosis. Gross pathological findings were peculiar; animals that died early had more chance of presenting severe jaundice and less chance of presenting pulmonary hemorrhages (P < 0.01). L. borgpetersenii serogroup Ballum has had a considerable growth in human leptospirosis cases in recent years. This strain has now been thoroughly characterized and can be used in more studies, especially evaluations of vaccine candidates.
Author Notes
Financial support: This work was supported by CNPq.
Authors' addresses: Juliana Alcoforado Diniz, Samuel Rodrigues Félix, Amilton Clair Pinto Seixas Neto, Flávia Aleixo Vasconcellos, André Alex Grassmann, Odir Antônio Dellagostin, José Antonio Guimarães Aleixo, and Éverton Fagonde da Silva, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil, E-mails: juju_adiniz@yahoo.com.br, samuelrf@gmail.com, amiltonseixas@gmail.com, eualevas@yahoo.com.br, grassmann.aa@gmail.com, odir@ufpel.edu.br, biotjaga@ufpel.tche.br, and efsilva@ufpel.edu.br. Josiane Bonel-Raposo, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil, E-mail: jbonel-raposo@hotmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Ricaldi JN, Vinetz JM, 2006. Leptospirosis in the tropics and in travelers. Curr Infect Dis Rep 8: 51–58.
-
2.
Ko AI, Goarant C, Picardeau M, 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7: 736–747.
-
3.
Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757–771.
-
4.
Gouveia EL, Metcalfe J, de Carvalho AL, Aires TS, Villasboas-Bisneto JC, Queirroz A, Santos AC, Salgado K, Reis MG, Ko AI, 2008. Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. Emerg Infect Dis 14: 505–508.
-
5.
Arean VM, 1962. Studies on the pathogenesis of leptospirosis. II. A clinicopathologic evaluation of hepatic and renal function in experimental leptospiral infections. Lab Invest 11: 273–288.
-
6.
Silva ÉF, Santos CS, Athanazio DA, Seyffert N, Seixas FK, Cerqueira GM, Fagundes MQ, Brod CS, Reis MG, Dellagostin OA, Ko AI, 2008. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine 26: 3892–3896.
-
7.
Sanger VL, Hamdy AH, Fizette WB, Bohl EH, Ferguson LC, 1961. Leptospira pomona infection in hamsters. Cornell Vet 51: 489–498.
-
8.
Badiola J, Thiermann AB, Cheville NF, 1983. Pathologic features of leptospirosis in hamsters caused by Leptospira interrogans serovars hardjo and szwajizak. Am J Vet Res 44: 91–99.
-
9.
Barnett JK, Barnett D, Bolin CA, Summers TA, Wagar EA, Cheville NF, Hartskeerl RA, Haake DA, 1999. Expression and distribution of leptospiral outer membrane components during renal infection of hamsters. Infect Immun 67: 853–861.
-
10.
Slack AT, Symonds ML, Dohnt MF, Craig SB, Smythe LD, 2010. Molecular Epidemiology of Leptospira borgpetersenii serovar Arborea, Queensland, Australia, 1998–2005. Am J Trop Med Hyg 83: 820–821.
-
11.
Herrmann Storck C, Postic D, Lamaury I, Perez JM, 2008. Changes in epidemiology of leptospirosis in 2003–2004, a two El Niño Southern Oscillation period, Guadeloupe archipelago, French West Indies. Epidemiol Infect 136: 1407–1415.
-
12.
Thornley CN, Baker MG, Weinstein P, Maas EW, 2002. Changing epidemiology of human leptospirosis in New Zealand. Epidemiol Infect 128: 29–36.
-
13.
Vieira ML, Gama-Simões MJ, Collares-Pereira M, 2006. Human leptospirosis in Portugal: a retrospective study of eighteen years. Int J Infect Dis 10: 378–386.
-
14.
Silva ÉF, Felix SR, Cerqueira GM, Fagundes MQ, Seixas Neto ACP, Grassmann AA, Amaral MG, Gallina T, Dellagostin OA, 2010. Preliminary characterization of Mus musculus-derived pathogenic strains of Leptospira borgpetersenii serogroup Ballum in a hamster model. Am J Trop Med Hyg 83: 336–337.
-
15.
Faine SB, Adler B, Bolin C, Perolat P, 1999. Leptospira and Leptospirosis. Melbourne, Australia: MediSci.
-
16.
Reed LJ, Muench H, 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493–497.
-
17.
Sonrier C, Branger C, Michel V, Ruvoën-Clouet N, Ganière JP, André-Fontaine G, 2000. Evidence of cross-protection within Leptospira interrogans in an experimental model. Vaccine 19: 86–94.
-
18.
Wang Z, Jin L, Wegrzyn A, 2007. Leptospirosis vaccines. Microb Cell Fact 6: 39–49.
-
19.
Palaniappan RUM, McDonough SP, Divers TJ, Chen CS, Pan MJ, Matsumoto M, Chang YF, 2006. Immunoprotection of recombinant Leptospiral immunoglobulin-like protein a against Leptospira interrogans serovar pomona infection. Infect Immun 74: 1745–1750.
-
20.
Chang YF, Chen CS, Palaniappan RUM, He H, McDonough SP, Barr SC, Yan W, Faisal SM, Pan MJ, Chang CF, 2007. Immunogenicity of the recombinant leptospiral putative outer membrane proteins as vaccine candidates. Vaccine 25: 8190–8197.
-
21.
Silva ÉF, Medeiros MA, McBride AJA, Matsunaga J, Esteves GS, Ramos JGR, Santos CS, Croda J, Homma A, Dellagostin OA, Haake DA, Reis MG, Ko AI, 2007. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine 25: 6277–6286.
-
22.
Faisal SM, Yan W, Chen C, Palaniappan RUM, McDonough SP, Chang YF, 2008. Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters. Vaccine 26: 277–287.
-
23.
Suepaul SM, Carrington CV, Campbell M, Borde G, Adesiyun AA, 2010. Study on the efficacy of Leptospira vaccines developed from serovars isolated from Trinidad and comparison with commercial vaccines using a hamster model. Vaccine 28: 5421–5426.
-
24.
Felix SR, Silva ÉF, Jouglard SDD, Hartmann DM, Grassmann AA, Dellagostin OA, 2009. Leptospirosis vaccine: search for subunit candidates. Proc Vaccinol 1: 110–114.
-
25.
Santos CS, Macedo JO, Bandeira M, Chagas-Junior AD, McBride AJ, McBride FW, Reis MG, Athanazio DA, 2010. Different outcomes of experimental leptospiral infection in mouse strains with distinct genotypes. J Med Microbiol 59: 1101–1106.
-
26.
Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, Davis J, Johnson M, Kuczek E, Alt DP, Peterson-Burch B, Coppel RL, Rood JI, Davies JK, Adler B, 2006. Genome reduction in Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci USA 103: 14560–14565.
-
27.
Nascimento ALTO, Ko AI, Martins EAL, Monteiro-Vitorello CB, Ho PL, Haake DA, 2004. Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186: 2164–2172.
-
28.
Adler B, Moctezuma AD, 2010. Leptospira and leptospirosis. Vet Microbiol 140: 287–296.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 22 | 22 | 6 |
| Full Text Views | 273 | 64 | 1 |
| PDF Downloads | 52 | 14 | 1 |