Multiplex Bead Assay for Serum Samples from Children in Haiti Enrolled in a Drug Study for the Treatment of Lymphatic Filariasis

Delynn M. Moss Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Jeffrey W. Priest Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Alexis Boyd Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Tiffany Weinkopff Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Zuzana Kucerova Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Michael J. Beach Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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Patrick J. Lammie Division of Parasitic Diseases, National Center for Zoonotic, Vector-borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

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A multiplex bead assay (MBA) was used to analyze serum samples collected longitudinally from children enrolled in a drug trial for treatment of filariasis in Leogane, Haiti. Recombinant antigens Bm14 and Bm33 from Brugia malayi, third polar tube protein (PTP3) from Encephalitozoon cuniculi, and merozoite surface protein-119 (MSP-119) from Plasmodium falciparum were coupled to carboxylated polystyrene microspheres. IgG responses to PTP3 and MSP-119 were not affected by albendazole (ALB), diethylcarbamazine (DEC), or combination of diethylcarbamazine and albendazole (DEC/ALB). However, IgG and IgG4 responses to Bm14 and Bm33 were significantly decreased (P < 0.001) by DEC and DEC/ALB treatment. Antibody responses to Bm14 and Bm33 decreased after DEC treatment (but not placebo) among children who were negative for microfilaremia and antigenemia at baseline, suggesting that these children harbored early stages of infection. The MBA is an excellent serologic technique for multiple antigens that offers substantial advantages over single-antigen based enzyme-linked immunosorbent assay in mass drug administration studies for monitoring changes in antibody levels.

Author Notes

*Address correspondence to Delynn M. Moss, Division of Parasitic Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30329. E-mail: dmm3@cdc.gov

Authors' addresses: Delynn M. Moss, Waterborne Disease Prevention Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: dmm3@cdc.gov. Jeffrey W. Priest, Waterborne Disease Prevention Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: jip8@cdc.gov. Alexis Boyd, Office of Intramural Training and Education, National Institutes of Health, Bethesda, MD, E-mail: boyda2@od.nih.gov. Tiffany Weinkopff, Zuzana Kucerova, and Patrick J. Lammie, Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: tiffany.weinkopff@unil.ch 1600, zik0@cdc.gov, and pjl1@cdc.gov. Michael J. Beach, Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: mjb3@cdc.gov.

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