Bureau of Land Management, 2010. U.S. Department of the Interior, Bureau of Land Management. National Wild Horse and Burro Program. Available at: http://www.blm.gov/wo/st/en/prog/wild_horse_and_burro/wh_b_information_center/Fact_Sheet.html. Accessed July 22, 2010.)| false
JohnsonAJ, MartinDA, KarabatsosN, RoehrigJT, 2000. Detection of anti-arboviral immunoglobin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J Clin Microbiol38: 1827–1831.
JohnsonAJMartinDAKarabatsosNRoehrigJT, 2000. Detection of anti-arboviral immunoglobin G by using a monoclonal antibody-based capture enzyme-linked immunosorbent assay. J Clin Microbiol38: 1827–1831.)| false
We screened 1,397 feral horses (Equus caballus) on Sheldon National Wildlife Refuge, Nevada, United States, for IgM and IgG against flavivirus during 2004–2006, 2008, and 2009. Positive serum samples were tested for neutralizing antibodies to West Nile virus (WNV) and St. Louis encephalitis virus (SLEV). One animal was positive for antibody against WNV in 2004, but all others tested in 2004–2006 were negative. In 2008 and 2009, we found evidence of increasing seropositive horses with age, whereas seroprevalence of WNV decreased from 19% in 2008 to 7.2% in 2009. No horses were positive for antibody against SLEV. Being unvaccinated, feral horses can be useful for WNV surveillance.
*Address correspondence to J. Christian Franson, U.S. Geological Survey, National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711. E-mail: email@example.com
Authors' addresses: J. Christian Franson, Erik K. Hofmeister, and Robert J. Dusek, U.S. Geological Survey, National Wildlife Health Center, Madison, WI, E-mails: firstname.lastname@example.org, email@example.com, and firstname.lastname@example.org. Gail H. Collins, U.S. Fish and Wildlife Service, Sheldon-Hart Mountain National Wildlife Refuge Complex, Lakeview, OR, E-mail: email@example.com.