Centers for Disease Control and Prevention, 1993. Recommendations of the International Task Force for Disease Eradication. MMWR Morb Mortal Wkly Rep 42: 1–25.
Schantz PM, Cruz M, Sarti E, Pawlowski Z, 1993. Potential eradicability of taeniasis and cysticercosis. Bull Pan Am Health Organ 27: 397–403.
Schantz PM, Tsang VC, 2003. The US Centers for Disease Control and Prevention (CDC) and research and control of cysticercosis. Acta Trop 87: 161–163.
Garcia HH, Gilman RH, Catacora M, Verastegui M, Gonzalez AE, Tsang VC, 1997. Serologic evolution of neurocysticercosis patients after antiparasitic therapy. Cysticercosis Working Group in Peru. J Infect Dis 175: 486–489.
Pan American Health Organization, 1995. PAHO/WHO Informal Consultation on the Taeniasis/Cysticercosis Complex. Brasilia, Brazil: Pan American Health Organization.
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159: 50–59.
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99: 257–261.
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86: 1001–1007.
Sako Y, Nakao M, Ikejima T, Piao XZ, Nakaya K, Ito A, 2000. Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. J Clin Microbiol 38: 4439–4444.
Obregon-Henao A, Gil DL, Gomez DI, Sanzon F, Teale JM, Restrepo BI, 2001. The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Mol Biochem Parasitol 114: 209–215.
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Tsang VC, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41: 2577–2586.
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133: 115–124.
Hancock K, Pattabhi S, Whitfield FW, Yushak ML, Lane WS, Garcia HH, Gonzalez AE, Gilman RH, Tsang VC, 2006. Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 147: 109–117.
Handali S, Gonzalez AE, Hancock K, Garcia HH, Roberts JM, Gilman RH, Tsang VC, 2004. Porcine antibody responses to Taenia solium antigens rGp50 and sTs18var1. Am J Trop Med Hyg 71: 322–326.
Bueno EC, Scheel CM, Vaz AJ, Machado LR, Livramento JA, Takayanagui OM, Tsang VC, Hancock K, 2005. Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. Am J Trop Med Hyg 72: 278–283.
Scheel CM, Khan A, Hancock K, Garcia HH, Gonzalez AE, Gilman RH, Tsang VC, 2005. Serodiagnosis of neurocysticercosis using synthetic 8-kD proteins: comparison of assay formats. Am J Trop Med Hyg 73: 771–776.
Del Brutto OH, Rajshekhar V, White AC Jr, Tsang VC, Nash TE, Takayanagui OM, Schantz PM, Evans CA, Flisser A, Correa D, Botero D, Allan JC, Sarti E, Gonzalez AE, Gilman RH, Garcia HH, 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology 57: 177–183.
Handali S, Pattabhi S, Lee YM, Silva-Ibanez M, Kovalenko VA, Levin AE, Gonzalez AE, Roberts JM, Garcia HH, Gilman RH, Hancock K, Tsang VC, 2010. Development andevaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer. J ImmunoassayImmunochem 1: 60–70.
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3: 32–35.
Diaz JF, Verastegui M, Gilman RH, Tsang VC, Pilcher JB, Gallo C, Garcia HH, Torres P, Montenegro T, Miranda E, 1992. Immunodiagnosis of human cysticercosis (Taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (ELISA), an antigen-ELISA, and an enzyme-linked immunoelectrotransfer blot (EITB) assay in Peru. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 46: 610–615.
Carpio A, 2002. Neurocysticercosis: an update. Lancet Infect Dis 2: 751–762.
Garcia HH, Gonzalez AE, Evans CA, Gilman RH, 2003. Taenia solium cysticercosis. Lancet 362: 547–556.
Montano SM, Villaran MV, Yiquimiche L, Figueroa JJ, Rodriguez S, Bautista CT, Gonzalez AE, Tsang VC, Gilman RH, Garica HH, 2005. Neurocysticercosis: association between seizures, serology, and brain CT in rural Peru. Neurology 65: 229–233.
Marconi VC, Garcia HH, Katz JT, 2006. Neurocysticercosis. Curr Infect Dis Rep 8: 293–300.
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, Tsang VC, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164: 1007–1009.
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Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.
Financial support: This study was supported by the Bill & Melinda Gates Foundation (grant no. 23981) and National Institutes of Health SIBR grants 1R43AI64988-01 and 2R44A1064988-02.
Authors' addresses: Yeuk-Mui Lee, Sukwan Handali, Kathy Hancock, Sowmya Pattabhi, and Christina M. Scheel, Division of Parasitic Diseases and Malaria, Centers for Diseases Control and Prevention, Atlanta, GA, E-mails: ylee@cdc.gov, SHandali@cdc.gov, KHancock@cdc.gov, spattabhi@idri.org, and CScheel@cdc.gov. Victor A. Kovalenko and Andrew Levin, Immunetics, Inc., Boston, MA, E-mails: vdiagnosticnno@maine.rr.com and alevin@Immunetics.com. Silvia Rodriguez and Hector H. Garcia, Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru and Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru, E-mails: silvia@peruresearch.com and hgarcia@jhsph.edu. Sehching Lin, Scientific Resource Program, Coordinating Center for Infectious Diseases, Center for Diseases Control and Prevention, Atlanta, GA, E-mail: SLin@cdc.gov. Armando E. Gonzalez, School of Veterinarian Medicine, Universidad de San Marcos, Lima, Peru, E-mail: agonzale@jhsph.edu. Robert H. Gilman, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, E-mail: rgilman@jhsph.edu. Victor C. W. Tsang, Division of Parasitic Diseases and Malaria, Centers for Diseases Control and Prevention, Atlanta, GA, and Department of Biology, Georgia State University, Atlanta, GA, E-mail: vcwtsang@mindspring.com.
Centers for Disease Control and Prevention, 1993. Recommendations of the International Task Force for Disease Eradication. MMWR Morb Mortal Wkly Rep 42: 1–25.
Schantz PM, Cruz M, Sarti E, Pawlowski Z, 1993. Potential eradicability of taeniasis and cysticercosis. Bull Pan Am Health Organ 27: 397–403.
Schantz PM, Tsang VC, 2003. The US Centers for Disease Control and Prevention (CDC) and research and control of cysticercosis. Acta Trop 87: 161–163.
Garcia HH, Gilman RH, Catacora M, Verastegui M, Gonzalez AE, Tsang VC, 1997. Serologic evolution of neurocysticercosis patients after antiparasitic therapy. Cysticercosis Working Group in Peru. J Infect Dis 175: 486–489.
Pan American Health Organization, 1995. PAHO/WHO Informal Consultation on the Taeniasis/Cysticercosis Complex. Brasilia, Brazil: Pan American Health Organization.
Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159: 50–59.
Greene RM, Wilkins PP, Tsang VC, 1999. Diagnostic glycoproteins of Taenia solium cysts share homologous 14- and 18-kDa subunits. Mol Biochem Parasitol 99: 257–261.
Greene RM, Hancock K, Wilkins PP, Tsang VC, 2000. Taenia solium: molecular cloning and serologic evaluation of 14- and 18-kDa related, diagnostic antigens. J Parasitol 86: 1001–1007.
Sako Y, Nakao M, Ikejima T, Piao XZ, Nakaya K, Ito A, 2000. Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. J Clin Microbiol 38: 4439–4444.
Obregon-Henao A, Gil DL, Gomez DI, Sanzon F, Teale JM, Restrepo BI, 2001. The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Mol Biochem Parasitol 114: 209–215.
Hancock K, Khan A, Williams FB, Yushak ML, Pattabhi S, Tsang VC, 2003. Characterization of the 8-kilodalton antigens of Taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. J Clin Microbiol 41: 2577–2586.
Hancock K, Pattabhi S, Greene RM, Yushak ML, Williams F, Khan A, Priest JW, Levine MZ, Tsang VC, 2004. Characterization and cloning of GP50, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 133: 115–124.
Hancock K, Pattabhi S, Whitfield FW, Yushak ML, Lane WS, Garcia HH, Gonzalez AE, Gilman RH, Tsang VC, 2006. Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis. Mol Biochem Parasitol 147: 109–117.
Handali S, Gonzalez AE, Hancock K, Garcia HH, Roberts JM, Gilman RH, Tsang VC, 2004. Porcine antibody responses to Taenia solium antigens rGp50 and sTs18var1. Am J Trop Med Hyg 71: 322–326.
Bueno EC, Scheel CM, Vaz AJ, Machado LR, Livramento JA, Takayanagui OM, Tsang VC, Hancock K, 2005. Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. Am J Trop Med Hyg 72: 278–283.
Scheel CM, Khan A, Hancock K, Garcia HH, Gonzalez AE, Gilman RH, Tsang VC, 2005. Serodiagnosis of neurocysticercosis using synthetic 8-kD proteins: comparison of assay formats. Am J Trop Med Hyg 73: 771–776.
Del Brutto OH, Rajshekhar V, White AC Jr, Tsang VC, Nash TE, Takayanagui OM, Schantz PM, Evans CA, Flisser A, Correa D, Botero D, Allan JC, Sarti E, Gonzalez AE, Gilman RH, Garcia HH, 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology 57: 177–183.
Handali S, Pattabhi S, Lee YM, Silva-Ibanez M, Kovalenko VA, Levin AE, Gonzalez AE, Roberts JM, Garcia HH, Gilman RH, Hancock K, Tsang VC, 2010. Development andevaluation of porcine cysticercosis QuickELISA in Triturus EIA analyzer. J ImmunoassayImmunochem 1: 60–70.
Youden WJ, 1950. Index for rating diagnostic tests. Cancer 3: 32–35.
Diaz JF, Verastegui M, Gilman RH, Tsang VC, Pilcher JB, Gallo C, Garcia HH, Torres P, Montenegro T, Miranda E, 1992. Immunodiagnosis of human cysticercosis (Taenia solium): a field comparison of an antibody-enzyme-linked immunosorbent assay (ELISA), an antigen-ELISA, and an enzyme-linked immunoelectrotransfer blot (EITB) assay in Peru. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 46: 610–615.
Carpio A, 2002. Neurocysticercosis: an update. Lancet Infect Dis 2: 751–762.
Garcia HH, Gonzalez AE, Evans CA, Gilman RH, 2003. Taenia solium cysticercosis. Lancet 362: 547–556.
Montano SM, Villaran MV, Yiquimiche L, Figueroa JJ, Rodriguez S, Bautista CT, Gonzalez AE, Tsang VC, Gilman RH, Garica HH, 2005. Neurocysticercosis: association between seizures, serology, and brain CT in rural Peru. Neurology 65: 229–233.
Marconi VC, Garcia HH, Katz JT, 2006. Neurocysticercosis. Curr Infect Dis Rep 8: 293–300.
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, Tsang VC, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164: 1007–1009.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 998 | 871 | 19 |
Full Text Views | 341 | 16 | 2 |
PDF Downloads | 133 | 15 | 2 |