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Serologic Diagnosis of Human Taenia solium Cysticercosis by Using Recombinant and Synthetic Antigens in QuickELISA™

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  • Division of Parasitic Diseases and Malaria, and Scientific Resource Program, Coordinating Center for Infectious Diseases, Center for Global Health, Centers for Diseases Control and Prevention, Atlanta, Georgia; Immunetics, Inc., Boston, Massachusetts; Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru; School of Veterinarian Medicine, Universidad de San Marcos, Lima, Peru; Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland; Department of Biology, Georgia State University, Atlanta, Georgia

Diagnosis of Taenia solium cysticercosis is an important component in the control and elimination of cysticercosis and taeniasis. New detection assays using recombinant and synthetic antigens originating from the lentil lectin-purified glycoproteins (LLGPs) of T. solium cysticerci were developed in a QuickELISA™ format. We analyzed a panel of 474 serum samples composed of 108 serum samples from donors with two or more viable cysts, 252 serum samples from persons with other parasitic infections, and 114 serum samples from persons with no documented illnesses. The sensitivities and specificities of T24H QuickELISA™, GP50 QuickELISA™, and Ts18var1 QuickELISA™ were 96.3% and 99.2%, 93.5% and 98.6%, and 89.8% and 96.4%, respectively, for detecting cases with multiple, viable cysts. T24H QuickELISA™ performs best among the three assays, and has sensitivity and specificity values comparable to those of the LLGP enzyme-linked immunosorbent blot. The QuickELISA™ are simple, rapid quantitative methods for detecting antibodies specific for T. solium cysticerci antigens.

Author Notes

*Address correspondence to Yeuk-Mui Lee, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, 1600 Clifton Road, Mailstop D-64, Atlanta, GA 30329. E-mail: ylee@cdc.gov

Financial support: This study was supported by the Bill & Melinda Gates Foundation (grant no. 23981) and National Institutes of Health SIBR grants 1R43AI64988-01 and 2R44A1064988-02.

Authors' addresses: Yeuk-Mui Lee, Sukwan Handali, Kathy Hancock, Sowmya Pattabhi, and Christina M. Scheel, Division of Parasitic Diseases and Malaria, Centers for Diseases Control and Prevention, Atlanta, GA, E-mails: ylee@cdc.gov, SHandali@cdc.gov, KHancock@cdc.gov, spattabhi@idri.org, and CScheel@cdc.gov. Victor A. Kovalenko and Andrew Levin, Immunetics, Inc., Boston, MA, E-mails: vdiagnosticnno@maine.rr.com and alevin@Immunetics.com. Silvia Rodriguez and Hector H. Garcia, Department of Microbiology, Universidad Peruana Cayetano Heredia, Lima, Peru and Cysticercosis Unit, Instituto de Ciencias Neurologicas, Lima, Peru, E-mails: silvia@peruresearch.com and hgarcia@jhsph.edu. Sehching Lin, Scientific Resource Program, Coordinating Center for Infectious Diseases, Center for Diseases Control and Prevention, Atlanta, GA, E-mail: SLin@cdc.gov. Armando E. Gonzalez, School of Veterinarian Medicine, Universidad de San Marcos, Lima, Peru, E-mail: agonzale@jhsph.edu. Robert H. Gilman, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, E-mail: rgilman@jhsph.edu. Victor C. W. Tsang, Division of Parasitic Diseases and Malaria, Centers for Diseases Control and Prevention, Atlanta, GA, and Department of Biology, Georgia State University, Atlanta, GA, E-mail: vcwtsang@mindspring.com.

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