Author:
ProCite
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Reference Manager
BibTeX
Zotero
EndNote
-
1.
Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V, Aikawa M, 1980. Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science 207: 71–73.
-
2.
Clyde DF, Most H, McCarthy VC, Vanderberg JP, 1973. Immunization of man against sporozite-induced falciparum malaria. Am J Med Sci 266: 169–177.
-
3.
Dame JB, Williams JL, McCutchan TF, Weber JL, Wirtz RA, Hockmeyer WT, Maloy WL, Haynes JD, Schneider I, Roberts D, Sanders GR, Reddy EP, Diggs CL, Miller LH, 1984. Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science 225: 593–599.
-
4.
Escalante A, Barrio E, Ayala FJ, 1995. The phylogeny of the Plasmodium species based on the circumsporozoite protein gene. Mol Biol Evol 12: 616–626.
-
5.
Arnot DE, Barnwell JW, Tam JP, Nussenzweig V, Nussenzweig RS, Enea V, 1985. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science 230: 815–818.
-
6.
Ancsin JB, Kisilevsky R, 2004. A binding site for highly sulfated heparan sulfate is identified in the N terminus of the circumsporozoite protein: significance for malarial sporozoite attachment to hepatocytes. J Biol Chem 279: 21824–21832.
-
7.
Sidjanski SP, Vanderberg JP, Sinnis P, 1997. Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum. Mol Biochem Parasitol 90: 33–41.
-
8.
Tewari R, Spaccapelo R, Bistoni F, Holder AA, Crisanti A, 2002. Function of region I and II adhesive motifs of Plasmodium falciparum circumsporozoite protein in sporozoite motility and infectivity. J Biol Chem 277: 47613–47618.
-
9.
Rosenberg R, Wirtz RA, Lanar DE, Sattabongkot J, Hall T, Waters AP, Prasittisuk C, 1989. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science 245: 973–976.
-
10.
Zakeri S, Abouie Mehrizi A, Djadid ND, Snounou G, 2006. Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran. Trop Med Int Health 11: 729–737.
-
11.
Gonzalez JM, Hurtado S, Arévalo-Herrera M, Herrera S, 2001. Variants of the Plasmodium vivax circumsporozoite protein (VK210 and VK247) in Colombian isolates. Mem Inst Oswaldo Cruz 96: 709–712.
-
12.
Kim T, Kim YJ, Song KJ, Song JW, Cha SH, Kim YK, Shin YK, Suh IB, Lim CS, 2002. The molecular characteristics of circumsporozoite protein gene subtypes from Plasmodium vivax isolates in Republic of Korea. Parasitol Res 88: 1051–1054.
-
13.
Mann VH, Huang T, Cheng Q, Saul A, 1994. Sequence variation in the circumsporozoite protein gene of Plasmodium vivax appears to be regionally biased. Mol Biochem Parasitol 68: 45–52.
-
14.
Qari SH, Goldman IF, Povoa MM, Oliveira S, Alpers MP, Lal AA, 1991. Wide distribution of the variant form of the human malaria parasite Plasmodium vivax. J Biol Chem 266: 16297–16300.
-
15.
Miller SA, Dykes DD, Polesky HF, 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215.
-
16.
Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315–320.
-
17.
Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
-
18.
Kho WG, Park YH, Chung JY, Kim JP, Hong ST, Lee WJ, Kim TS, Lee JS, 1999. Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea. Korean J Parasitol 37: 265–270.
-
19.
Librado P, Rozas J, 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451–1452.
-
20.
Herrera S, Escobar P, de Plata C, Avila GI, Corradin G, Herrera MA, 1992. Human recognition of T cell epitopes on the Plasmodium vivax circumsporozoite protein. J Immunol 148: 3986–3990.
-
21.
Arévalo-Herrera M, Roggero MA, Gonzalez JM, Vergara J, Corradin G, Lopez JA, Herrera S, 1998. Mapping and comparison of the B-cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys. Ann Trop Med Parasitol 92: 539–551.
-
22.
Arévalo-Herrera M, Valencia AZ, Vergara J, Bonelo A, Fleischhauer K, Gonzalez JM, Restrepo JC, Lopez JA, Valmori D, Corradin G, Herrera S, 2002. Identification of HLA-A2 restricted CD8(+) T-lymphocyte responses to Plasmodium vivax circumsporozoite protein in individuals naturally exposed to malaria. Parasite Immunol 24: 161–169.
-
23.
Escalante AA, Grebert HM, Isea R, Goldman IF, Basco L, Magris M, Biswas S, Kariuki S, Lal AA, 2002. A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas-XVI. Asembo Bay Cohort Project. Mol Biochem Parasitol 125: 83–90.
-
24.
Escalante AA, Lal AA, Ayala FJ, 1998. Genetic polymorphism and natural selection in the malaria parasite Plasmodium falciparum. Genetics 149: 189–202.
-
25.
Kumkhaek C, Phra-Ek K, Renia L, Singhasivanon P, Looareesuwan S, Hirunpetcharat C, White NJ, Brockman A, Gruner AC, Lebrun N, Alloueche A, Nosten F, Khusmith S, Snounou G, 2005. Are extensive T cell epitope polymorphisms in the Plasmodium falciparum circumsporozoite antigen, a leading sporozoite vaccine candidate, selected by immune pressure? J Immunol 175: 3935–3939.
-
26.
Arnot DE, Stewart MJ, Barnwell JW, 1990. Antigenic diversity in Thai Plasmodium vivax circumsporozoite proteins. Mol Biochem Parasitol 43: 147–149.
-
27.
Levinson G, Gutman GA, 1987. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4: 203–221.
-
28.
Arnot DE, Barnwell JW, Stewart MJ, 1988. Does biased gene conversion influence polymorphism in the circumsporozoite protein-encoding gene of Plasmodium vivax? Proc Natl Acad Sci USA 85: 8102–8106.
-
29.
Hughes AL, 2004. The evolution of amino acid repeat arrays in Plasmodium and other organisms. J Mol Evol 59: 528–535.
-
30.
Hughes AL, 1991. Circumsporozoite protein genes of malaria parasites (Plasmodium spp.): evidence for positive selection on immunogenic regions. Genetics 127: 345–353.
-
31.
McCutchan TF, Good MF, Miller LH, 1989. Polymorphism in the circumsporozoite (CS) protein of Plasmodium falciparum. Parasitol Today 5: 143–146.
-
32.
Shi YP, Alpers MP, Povoa MM, Lal AA, 1992. Diversity in the immunodominant determinants of the circumsporozoite protein of Plasmodium falciparum parasites from malaria-endemic regions of Papua New Guinea and Brazil. Am J Trop Med Hyg 47: 844–851.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 27 | 27 | 8 |
| Full Text Views | 403 | 175 | 0 |
| PDF Downloads | 79 | 24 | 0 |
Antigenic Diversity of the Plasmodium vivax Circumsporozoite Protein in Parasite Isolates of Western Colombia
Miguel Ángel Hernández-Martínez
Miguel Ángel Hernández-Martínez
Malaria Vaccine and Drug Development Center, Cali, Colombia; Fundación Centro de Primates, Cali, Colombia; Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia; School of Life Sciences, Arizona State University, Tempe, Arizona
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Ananías A. Escalante
Ananías A. Escalante
Malaria Vaccine and Drug Development Center, Cali, Colombia; Fundación Centro de Primates, Cali, Colombia; Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia; School of Life Sciences, Arizona State University, Tempe, Arizona
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Myriam Arévalo-Herrera
Myriam Arévalo-Herrera
Malaria Vaccine and Drug Development Center, Cali, Colombia; Fundación Centro de Primates, Cali, Colombia; Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia; School of Life Sciences, Arizona State University, Tempe, Arizona
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Sócrates Herrera
Sócrates Herrera
Malaria Vaccine and Drug Development Center, Cali, Colombia; Fundación Centro de Primates, Cali, Colombia; Instituto de Inmunología del Valle, Universidad del Valle, Cali, Colombia; School of Life Sciences, Arizona State University, Tempe, Arizona
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Circumsporozoite (CS) protein is a malaria antigen involved in sporozoite invasion of hepatocytes, and thus considered to have good vaccine potential. We evaluated the polymorphism of the Plasmodium vivax CS gene in 24 parasite isolates collected from malaria-endemic areas of Colombia. We sequenced 27 alleles, most of which (25/27) corresponded to the VK247 genotype and the remainder to the VK210 type. All VK247 alleles presented a mutation (Gly → Asn) at position 28 in the N-terminal region, whereas the C-terminal presented three insertions: the ANKKAGDAG, which is common in all VK247 isolates; 12 alleles presented the insertion GAGGQAAGGNAANKKAGDAG; and 5 alleles presented the insertion GGNAGGNA. Both repeat regions were polymorphic in gene sequence and size. Sequences coding for B-, T-CD4+, and T-CD8+ cell epitopes were found to be conserved. This study confirms the high polymorphism of the repeat domain and the highly conserved nature of the flanking regions.
Author Notes
Financial support: This work was supported by grants from National Institute of Health (NIH), the Instituto Colombiano Francisco José de Caldas para la Ciencia y la Tecnología COLCIENCIAS, and the Colombian Ministry for Social Protection (contract no. 216-2006) and through an International Center of Excellence for Malaria Research NIAID/ICEMR grant no U 19AI089702.
Authors' addresses: Miguel Ángel Hernández Martínez, Myriam Arévalo-Herrera, and Sócrates Herrera, Instituto de Inmunología, Edificio de Microbiología, Facultad de Salud, Universidad del Valle and Centro Internacional de Vacunas, Cali, Colombia, E-mails: miangher@hotmail.com, marevalo@inmuno.org, and sherrera@inmuno.org. Ananías A. Escalante, School of Life Sciences, Arizona State University, Tempe, AZ, E-mail: Ananias.Escalante@asu.edu.
Reprint requests: Sócrates Herrera, Malaria Vaccine and Drug Development Center, Carrera 37 - 2Bis No. 5E - 08, Cali, Colombia, E-mail: sherrera@inmuno.org.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Yoshida N, Nussenzweig RS, Potocnjak P, Nussenzweig V, Aikawa M, 1980. Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite. Science 207: 71–73.
-
2.
Clyde DF, Most H, McCarthy VC, Vanderberg JP, 1973. Immunization of man against sporozite-induced falciparum malaria. Am J Med Sci 266: 169–177.
-
3.
Dame JB, Williams JL, McCutchan TF, Weber JL, Wirtz RA, Hockmeyer WT, Maloy WL, Haynes JD, Schneider I, Roberts D, Sanders GR, Reddy EP, Diggs CL, Miller LH, 1984. Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum. Science 225: 593–599.
-
4.
Escalante A, Barrio E, Ayala FJ, 1995. The phylogeny of the Plasmodium species based on the circumsporozoite protein gene. Mol Biol Evol 12: 616–626.
-
5.
Arnot DE, Barnwell JW, Tam JP, Nussenzweig V, Nussenzweig RS, Enea V, 1985. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science 230: 815–818.
-
6.
Ancsin JB, Kisilevsky R, 2004. A binding site for highly sulfated heparan sulfate is identified in the N terminus of the circumsporozoite protein: significance for malarial sporozoite attachment to hepatocytes. J Biol Chem 279: 21824–21832.
-
7.
Sidjanski SP, Vanderberg JP, Sinnis P, 1997. Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum. Mol Biochem Parasitol 90: 33–41.
-
8.
Tewari R, Spaccapelo R, Bistoni F, Holder AA, Crisanti A, 2002. Function of region I and II adhesive motifs of Plasmodium falciparum circumsporozoite protein in sporozoite motility and infectivity. J Biol Chem 277: 47613–47618.
-
9.
Rosenberg R, Wirtz RA, Lanar DE, Sattabongkot J, Hall T, Waters AP, Prasittisuk C, 1989. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science 245: 973–976.
-
10.
Zakeri S, Abouie Mehrizi A, Djadid ND, Snounou G, 2006. Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran. Trop Med Int Health 11: 729–737.
-
11.
Gonzalez JM, Hurtado S, Arévalo-Herrera M, Herrera S, 2001. Variants of the Plasmodium vivax circumsporozoite protein (VK210 and VK247) in Colombian isolates. Mem Inst Oswaldo Cruz 96: 709–712.
-
12.
Kim T, Kim YJ, Song KJ, Song JW, Cha SH, Kim YK, Shin YK, Suh IB, Lim CS, 2002. The molecular characteristics of circumsporozoite protein gene subtypes from Plasmodium vivax isolates in Republic of Korea. Parasitol Res 88: 1051–1054.
-
13.
Mann VH, Huang T, Cheng Q, Saul A, 1994. Sequence variation in the circumsporozoite protein gene of Plasmodium vivax appears to be regionally biased. Mol Biochem Parasitol 68: 45–52.
-
14.
Qari SH, Goldman IF, Povoa MM, Oliveira S, Alpers MP, Lal AA, 1991. Wide distribution of the variant form of the human malaria parasite Plasmodium vivax. J Biol Chem 266: 16297–16300.
-
15.
Miller SA, Dykes DD, Polesky HF, 1988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16: 1215.
-
16.
Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315–320.
-
17.
Sambrook J, Fritsch EF, Maniatis T, 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
-
18.
Kho WG, Park YH, Chung JY, Kim JP, Hong ST, Lee WJ, Kim TS, Lee JS, 1999. Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea. Korean J Parasitol 37: 265–270.
-
19.
Librado P, Rozas J, 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451–1452.
-
20.
Herrera S, Escobar P, de Plata C, Avila GI, Corradin G, Herrera MA, 1992. Human recognition of T cell epitopes on the Plasmodium vivax circumsporozoite protein. J Immunol 148: 3986–3990.
-
21.
Arévalo-Herrera M, Roggero MA, Gonzalez JM, Vergara J, Corradin G, Lopez JA, Herrera S, 1998. Mapping and comparison of the B-cell epitopes recognized on the Plasmodium vivax circumsporozoite protein by immune Colombians and immunized Aotus monkeys. Ann Trop Med Parasitol 92: 539–551.
-
22.
Arévalo-Herrera M, Valencia AZ, Vergara J, Bonelo A, Fleischhauer K, Gonzalez JM, Restrepo JC, Lopez JA, Valmori D, Corradin G, Herrera S, 2002. Identification of HLA-A2 restricted CD8(+) T-lymphocyte responses to Plasmodium vivax circumsporozoite protein in individuals naturally exposed to malaria. Parasite Immunol 24: 161–169.
-
23.
Escalante AA, Grebert HM, Isea R, Goldman IF, Basco L, Magris M, Biswas S, Kariuki S, Lal AA, 2002. A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas-XVI. Asembo Bay Cohort Project. Mol Biochem Parasitol 125: 83–90.
-
24.
Escalante AA, Lal AA, Ayala FJ, 1998. Genetic polymorphism and natural selection in the malaria parasite Plasmodium falciparum. Genetics 149: 189–202.
-
25.
Kumkhaek C, Phra-Ek K, Renia L, Singhasivanon P, Looareesuwan S, Hirunpetcharat C, White NJ, Brockman A, Gruner AC, Lebrun N, Alloueche A, Nosten F, Khusmith S, Snounou G, 2005. Are extensive T cell epitope polymorphisms in the Plasmodium falciparum circumsporozoite antigen, a leading sporozoite vaccine candidate, selected by immune pressure? J Immunol 175: 3935–3939.
-
26.
Arnot DE, Stewart MJ, Barnwell JW, 1990. Antigenic diversity in Thai Plasmodium vivax circumsporozoite proteins. Mol Biochem Parasitol 43: 147–149.
-
27.
Levinson G, Gutman GA, 1987. Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4: 203–221.
-
28.
Arnot DE, Barnwell JW, Stewart MJ, 1988. Does biased gene conversion influence polymorphism in the circumsporozoite protein-encoding gene of Plasmodium vivax? Proc Natl Acad Sci USA 85: 8102–8106.
-
29.
Hughes AL, 2004. The evolution of amino acid repeat arrays in Plasmodium and other organisms. J Mol Evol 59: 528–535.
-
30.
Hughes AL, 1991. Circumsporozoite protein genes of malaria parasites (Plasmodium spp.): evidence for positive selection on immunogenic regions. Genetics 127: 345–353.
-
31.
McCutchan TF, Good MF, Miller LH, 1989. Polymorphism in the circumsporozoite (CS) protein of Plasmodium falciparum. Parasitol Today 5: 143–146.
-
32.
Shi YP, Alpers MP, Povoa MM, Lal AA, 1992. Diversity in the immunodominant determinants of the circumsporozoite protein of Plasmodium falciparum parasites from malaria-endemic regions of Papua New Guinea and Brazil. Am J Trop Med Hyg 47: 844–851.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 27 | 27 | 8 |
| Full Text Views | 403 | 175 | 0 |
| PDF Downloads | 79 | 24 | 0 |