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Human Trichostrongylus colubriformis Infection in a Rural Village in Laos

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  • Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Laboratório de Parasitologia, Escola de Medicina Veterinária e Zootecnia, Universidade Federal do Tocantins, Araguaína, Tocantins, Brazil; Station of Malariology, Parasitology and Entomology of Savannakhet Province, Savannakhet Province, Laos; National Institute of Public Health, Ministry of Health, Vientiane, Laos; Research Institute for Humanity and Nature, Kyoto, Japan

In Lahanam Village, Savannakhet Province, Laos, 125 of 253 villagers (49.4%) were found by fecal examination to harbor hookworm eggs. The eggs were heterogeneous in morphology and size, suggesting infections of mixed nematode species. To confirm the hookworm egg species, on a voluntary basis, 46 hookworm egg–positive participants were treated with albendazole, and post-treatment adult worms were collected from purged fecal samples. The common human hookworm was found in only 3 participants; 1 case of Necator americanus, and 2 cases of Ancylostoma duodenale. In contrast, adult Trichostrongylus worms were expelled from most participants (43 of 46, 93.5%). The Trichostrongylus species were confirmed by morphology and internal transcribed spacer 2 sequences; all worms were of the same species (T. colubriformis). In addition, some Trichostrongylus worms were obtained from a goat in the same village and identified as T. colubriformis. The results suggested that T. colubriformis was the main zoonotic species causing hookworm infections in the village.

Author Notes

*Address correspondence to Jitra Waikagul, Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400 Thailand. E-mail: tmjwk@mahidol.ac.th

Financial support: This study was supported by the Research Institute for Humanity and Nature research project Environmental Changes and Infectious Diseases in Tropical Asia (Kazuhiko Moji) and by the Faculty of Tropical Medicine, Mahidol University (Jitra Waikagul).

Authors' addresses: Megumi Sato, Laboratório de Parasitologia, Escola de Medicina Veterinária e Zootecnia, Universidade Federal do Tocantins, Araguaína, Tocantins, Brazil, E-mail: mi_197439@hotmail.com. Tippayarat Yoonuan, Surapol Sanguankiat, Supaporn Nuamtanong, and Jitra Waikagul, Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, E-mails: teesuphanat@yahoo.com, tmsurapon@mahidol.ac.th, tmsnt@mahidol.ac.th, and tmjwk@mahidol.ac.th. Tiengkham Pongvongsa, Inthava Phimmayoi, and Vilayphone Phanhanan, Station of Malariology, Parasitology and Entomology, North Phonesavang Village, Kaysone District, Savannakhet Province, Laos, E-mail: tpongvongsa@yahoo.com. Boungnong Boupha, National Institute of Public Health, Ministry of Health, Vientiane, Laos, E-mail: laoniph@laotel.com. Kazuhiko Moji, Research Institute for Humanity and Nature, 457-4 Motoyama, Kamigamo, Kita-ku, Kyoto, Japan, E-mail: moji-k@chikyu.ac.jp.

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