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Evaluation of Three Commercially Available Japanese Encephalitis Virus IgM Enzyme-Linked Immunosorbent Assays

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  • Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, Colorado; Expanded Programme on Immunization/Immunization, Vaccines and Biologicals Department/Family and Child Health Cluster/World Health Organization, Geneva, Switzerland; Department of Neurovirology, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bangalore, India; Immunization and Vaccine Development, World Health Organization–South-East Asia Regional Office (WHO–SEARO), New Delhi, India; National Polio and Measles Laboratory, IPH, Dhaka, Bangladesh; Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases, Global Immunization Division, Atlanta, Georgia
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We evaluated performance of three commercial Japanese encephalitis virus (JEV) IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) kits with a panel of serological specimens collected during a surveillance project of acute encephalitis syndrome in India and acute meningitis and encephalitis syndrome in Bangladesh. The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. The CDC results and specimen classifications were considered the reference standard. All three commercial kits had high specificity (95–99.5%), but low sensitivities, ranging from 17–57%, with both serum and cerebrospinal fluid samples. Specific factors contributing to low sensitivity compared with the CDC ELISA could not be determined through further analysis of the limits and dilution end points of IgM detection.

Author Notes

*Address correspondence to Jaimie S. Robinson, 3150 Rampart Rd., Fort Collins, CO 80521. E-mail: fxa3@cdc.gov

Authors' addresses: Jaimie S. Robinson, Brad J. Biggerstaff, and Barbara W. Johnson, Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, CO, E-mails: fxa3@cdc.gov, BBiggerstaff@cdc.gov, and bfj9@cdc.gov. David Featherstone, Expanded Programme on Immunization/Immunization, Vaccines and Biologicals Department/Family and Child Health Cluster/World Health Organization, Geneva, Switzerland, E-mail: featherstoned@who.int. Ravi Vasanthapuram and Anita Desai, Department of Neurovirology, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bangalore, India, E-mails: virusravi@gmail.com and adesai@gmail.com. Nalini Ramamurty, World Health Organization - South-East Asia Regional Office I.P. Estate, New Delhi, India, E-mail: Ramamurtyn@SEARO.WHO.INT. Anwarul Haque Chowdhury, National Polio and Measles Laboratory, IPH, Dhaka, Bangladesh, E-mail: banplab@gmail.com. Hardeep S. Sandhu and Kathleen F. Cavallaro, CORP Bldg, Atlanta, GA, E-mails: hsandhu@cdc.gov and kfc1@cdc.gov.

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