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Immunoblot Diagnostic Test for Neurognathostomiasis

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  • Department of Parasitology, Radiology, and Medicine, Faculty of Medicine, and Research and Diagnosis Center for Emerging Infectious Disease, Khon Kaen University, Khon Kaen, Thailand

Neurognathostomiasis is a rare but severe form of human gnathostomiasis. Diagnosis of neurognathostomiasis is made presumably by using clinical manifestations. Serologic tests for neurognathostomiasis are not widely available and limited. We studied 12 patients with diagnoses of neurognathostomiasis at Srinagarind Hospital, Khon Kaen University, Thailand. There were three types of neurognathostomiasis (five patients with intracerebral hemorrhage, one patient with subarachnoid hemorrhage, and six patients with myelitis). All patients were tested for antibodies against Gnathostoma spinigerum by an immunoblotting technique. The sensitivity and specificity of the 21-kD and 24-kD diagnostic bands were 83.3% and 100%, and 91.7% and 100%, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value for the 21-kD and 24-kD diagnostic bands were all 100%. Both diagnostic bands are a helpful diagnostic tool for neuro gnathostomiasis and show good diagnostic properties.

Author Notes

*Address correspondence to Kittisak Sawanyawisuth, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: kittisak@kku.ac.th

Authors' addresses: Pewpan M. Intapan and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, and Research and Diagnosis Center for Emerging Infectious Disease, Khon Kaen University, Khon Kaen, 40002, Thailand, E-mails: pewpan@kku.ac.th and wanch_ma@kku.ac.th. Piyarat Khotsri, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand, E-mail: bewocto@yahoo.com. Jaturat Kanpittaya, Department of Radiology, Faculty of Medicine, and Diagnosis Center for Emerging Infectious Disease, Khon Kaen University, Khon Kaen, Thailand, E-mail: jatkan@gmail.com. Verajit Chotmongkol and Kittisak Sawanyawisuth, Department of Medicine, Faculty of Medicine, and Research and Diagnosis Center for Emerging Infectious Disease, Khon Kaen University, Khon Kaen, Thailand, E-mails: chotmongkolverajit@yahoo.com and kittisak@kku.ac.th.

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