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Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans

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  • Department of Virology, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain; Virology, Medicine School, Universidad de Valparaíso, Valparaiso, Chile; Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York; Service of Microbiology, Universitary Hospital “Virgen de las Nieves,” Service of Parasitology, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain; 454 Life Sciences, Branford, Connecticut

A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).

Author Notes

*Address correspondence to Maria Paz Sánchez-Seco, Laboratory of Arbovirus and Imported Viral Diseases, National Center of Microbiology, Institute of Health “Carlos III,” Ctra Pozuelo-Majadahonda km 2, 28220 Madrid, Spain. E-mail: paz.sanchez@isciii.es†These authors contributed equally.

Authors' addresses: Ximena Collao, Antonio Tenorio, and María Paz Sánchez-Seco, Laboratory of Arbovirus and Imported Viral Diseases, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mails: ximecf1@hotmail.com, atenorio@isciii.es, and paz.sanchez@isciii.es. Gustavo Palacios and W. Ian Lipkin, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, E-mails: gp2050@columbia.edu and wil2001@columbia.edu. Fernando de Ory, Serology Section, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mail: fory@isciii.es. Sara Sanbonmatsu, Mercedes Pérez-Ruiz, and José María Navarro, Service of Microbiology, Universitary Hospital “Virgen de las Nieve,” Granada, Spain, E-mails: sbmsara@yahoo.es, mercedes.perez.ruiz.sspa@juntadeandalucia.es, and josem.navarro.sspa@juntadeandalucia.es. Ricardo Molina, Service of Parasitology, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mail: rmolina@isciii.es. Stephen K. Hutchison, 454 Life Sciences, Branford, CT, E-mail: stephen.hutchison@roche.com. Ximena Collao actual address: Virology, Medicine School, Universidad de Valparaíso, Valparaiso, Chile.

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