ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Flamand M, Megret F, Mathieu M, Lepault J, Rey FA, Deubel V, 1999. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol 73: 6104–6110.
-
2.
Falconar AK, 1997. The dengue virus nonstructural-1 protein (NS1) generates antibodies to common epitopes on human blood clotting, integrin/adhesin proteins and binds to human endothelial cells: potential implications in haemorrhagic fever pathogenesis. Arch Virol 142: 897–916.
-
3.
Young R, Hilditch PA, Bletchly C, Halloran W, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
4.
Libraty DH, Young PR, Pickering D, Endy TP, Kalayanarooj S, Green S, Vaughn DW, Nisalak A, Ennis FA, Rothman AL, 2002. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis 186: 165–168.
-
5.
Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan K, Jairungsri A, Kanlaya R, Tangthawornchaikul N, Puttikhunt C, Pattanakitsakul SN, Yenchitsomanus PT, Mongkolsapaya J, Kasinrerk W, Sittisombut N, Husmann M, Blettner M, Vasanawathana S, Bhakdi S, Malasit P, 2006. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis 193: 1078–1088.
-
6.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.
-
7.
Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP, 2009. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3: e360.
-
8.
Thomas L, Verlaeten O, Cabié A, Kaidomar S, Moravie V, Martial J, Najioullah F, Plumelle Y, Fonteau C, Dussart P, Césaire R, 2008. Influence of the dengue serotype, previous dengue infection, and plasma viral load on clinical presentation and outcome during a dengue-2 and dengue-4 co-epidemic. Am J Trop Med Hyg 78: 990–998.
-
9.
World Health Organization, 1997. Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention and Control. Second edition. Geneva: World Health Organization.
-
10.
Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV, 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545–551.
-
11.
Vazquez S, Hafner G, Ruiz D, Calzada N, Guzman MG, 2007. Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. J Clin Virol 39: 194–198.
-
12.
Halstead SB, 1988. Pathogenesis of dengue: challenges to molecular biology. Science 239: 476–481.
-
13.
Vaughn DW, Green S, Kalayanarooj S, Innis BL, Nimmannitya S, Suntayakorn S, Endy TP, Raengsakulrach B, Rothman AL, Ennis FA, Nisalak A, 2000. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis 181: 2–9.
-
14.
Wang WK, Chen HL, Yang CF, Hsieh SC, Juan CC, Chang SM, Yu CC, Lin LH, Huang JH, King CC, 2006. Slower rates of clearance of viral load and virus-containing immune complexes in patients with dengue hemorrhagic fever. Clin Infect Dis 43: 1023–1030.
-
15.
Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH, 2003. Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10: 622–630.
-
16.
Koraka P, Burghoorn-Maas CP, Falconar A, Setiati TE, Djamiatun K, Groen J, Osterhaus AD, 2003. Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections. J Clin Microbiol 41: 4154–4159.
-
17.
Lapphra K, Sangcharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T, Yoksan S, Wattanamongkolsil L, Thamlikitkul V, 2008. Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis 60: 387–391.
-
18.
Martina BE, Koraka P, Osterhaus AD, 2009. Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev 22: 564–581.
-
19.
World Health Organization, 2009. Dengue. Guidelines for Diagnosis, Treatment, Prevention and Control. Third edition. Geneva: World Health Organization.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 997 | 787 | 12 |
| Full Text Views | 376 | 12 | 0 |
| PDF Downloads | 135 | 19 | 0 |
Relationship between Nonstructural Protein 1 Detection and Plasma Virus Load in Dengue Patients
Laurent Thomas
Laurent Thomas
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Fatiha Najioullah
Fatiha Najioullah
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Olivier Verlaeten
Olivier Verlaeten
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Jenny Martial
Jenny Martial
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Ségolène Brichler
Ségolène Brichler
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Stéphane Kaidomar
Stéphane Kaidomar
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Victor Moravie
Victor Moravie
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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André Cabié
André Cabié
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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Raymond Césaire
Raymond Césaire
Centre Hospitalier Universitaire, Fort-de-France, Martinique; Centre d'Investigation Clinique et d'Epidémiologie Clinique Antilles Guyane (CIC-EC INSERM U802), Fort-de-France, Martinique
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We report data from a prospective observational study performed in Martinique during a co-epidemic of dengue virus serotype 2 (DENV-2) and serotype 4 (DENV-4). Among 70 serum samples from patients with DENV-2 (n = 21) or DENV-4 (n = 49) infections, 47 (67.1%) were positive for dengue nonstructural protein 1 (NS1). Antigenemia correlated with plasma virus load and was independent of immune status and the time of sampling. Increased viremia 4–6 days after onset of illness was associated with NS1 positivity, secondary infection, and severe disease. Testing for NS1 could help identify the potentially most severely ill patients during the critical phase of dengue.
Author Notes
Authors' addresses: Laurent Thomas, Stéphane Kaidomar, and Victor Moravie, Service des Urgences, Centre Hospitalier Universitaire, Fort-de-France, Martinique. Fatiha Najioullah, Olivier Verlaeten, Jenny Martial, Ségolène Brichler, and Raymond Césaire, Laboratoire de Virologie-Immunologie, Centre Hospitalier Universitaire, Fort-de-France, Martinique. André Cabié, Service des Maladies Infectieuses et Tropicales, Centre Hospitalier Universitaire, Fort-de-France, Martinique.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Flamand M, Megret F, Mathieu M, Lepault J, Rey FA, Deubel V, 1999. Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion. J Virol 73: 6104–6110.
-
2.
Falconar AK, 1997. The dengue virus nonstructural-1 protein (NS1) generates antibodies to common epitopes on human blood clotting, integrin/adhesin proteins and binds to human endothelial cells: potential implications in haemorrhagic fever pathogenesis. Arch Virol 142: 897–916.
-
3.
Young R, Hilditch PA, Bletchly C, Halloran W, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
4.
Libraty DH, Young PR, Pickering D, Endy TP, Kalayanarooj S, Green S, Vaughn DW, Nisalak A, Ennis FA, Rothman AL, 2002. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J Infect Dis 186: 165–168.
-
5.
Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan K, Jairungsri A, Kanlaya R, Tangthawornchaikul N, Puttikhunt C, Pattanakitsakul SN, Yenchitsomanus PT, Mongkolsapaya J, Kasinrerk W, Sittisombut N, Husmann M, Blettner M, Vasanawathana S, Bhakdi S, Malasit P, 2006. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis 193: 1078–1088.
-
6.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.
-
7.
Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP, 2009. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3: e360.
-
8.
Thomas L, Verlaeten O, Cabié A, Kaidomar S, Moravie V, Martial J, Najioullah F, Plumelle Y, Fonteau C, Dussart P, Césaire R, 2008. Influence of the dengue serotype, previous dengue infection, and plasma viral load on clinical presentation and outcome during a dengue-2 and dengue-4 co-epidemic. Am J Trop Med Hyg 78: 990–998.
-
9.
World Health Organization, 1997. Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention and Control. Second edition. Geneva: World Health Organization.
-
10.
Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV, 1992. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 30: 545–551.
-
11.
Vazquez S, Hafner G, Ruiz D, Calzada N, Guzman MG, 2007. Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections. J Clin Virol 39: 194–198.
-
12.
Halstead SB, 1988. Pathogenesis of dengue: challenges to molecular biology. Science 239: 476–481.
-
13.
Vaughn DW, Green S, Kalayanarooj S, Innis BL, Nimmannitya S, Suntayakorn S, Endy TP, Raengsakulrach B, Rothman AL, Ennis FA, Nisalak A, 2000. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis 181: 2–9.
-
14.
Wang WK, Chen HL, Yang CF, Hsieh SC, Juan CC, Chang SM, Yu CC, Lin LH, Huang JH, King CC, 2006. Slower rates of clearance of viral load and virus-containing immune complexes in patients with dengue hemorrhagic fever. Clin Infect Dis 43: 1023–1030.
-
15.
Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH, 2003. Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10: 622–630.
-
16.
Koraka P, Burghoorn-Maas CP, Falconar A, Setiati TE, Djamiatun K, Groen J, Osterhaus AD, 2003. Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections. J Clin Microbiol 41: 4154–4159.
-
17.
Lapphra K, Sangcharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T, Yoksan S, Wattanamongkolsil L, Thamlikitkul V, 2008. Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis 60: 387–391.
-
18.
Martina BE, Koraka P, Osterhaus AD, 2009. Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev 22: 564–581.
-
19.
World Health Organization, 2009. Dengue. Guidelines for Diagnosis, Treatment, Prevention and Control. Third edition. Geneva: World Health Organization.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 997 | 787 | 12 |
| Full Text Views | 376 | 12 | 0 |
| PDF Downloads | 135 | 19 | 0 |