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-
1.
Gubler DJ, Meltzer M, 1999. Impact of dengue/dengue hemorrhagic fever on the developing world. Adv Virus Res 53: 35–70.
-
2.
World Health Organization, 2000. Dengue haemorrhagic fever: diagnosis, treatment and control. Handbook of the World Health Organization. Geneva: WHO, 1–84.
-
3.
Innis BL, 1995. Dengue and dengue haemorrhagic fever. Porterfield JS, ed. Exotic Viral Infections. London: Chapman & Hall Medical, 103–140.
-
4.
Blacksell SD, Mammen MP, Thongpaseuth S, Gibbons RV, Jarman RG, Jenjaroen K, Nisalak A, Phetsouvanh R, Newton PN, Day NP, 2008. Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis 60: 43–49.
-
5.
Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, Storck-Hermann C, Cesaire R, Morvan J, Flamand M, Baril L, 2006. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol 13: 1185–1189.
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6.
Kumarasamy V, Wahab AH, Chua SK, Hassan Z, Chem YK, Mohamad M, Chua KB, 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods 140: 75–79.
-
7.
McBride W, 2009. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis 64: 31–36.
-
8.
Sekaran SD, Lan EC, Mahesawarappa KB, Appanna R, Subramaniam G, 2007. Evaluation of a dengue NS1 capture ELISA assay for the rapid detection of dengue. J Infect Dev Ctries 1: 182–188.
-
9.
Young PR, Hilditch PA, Bletchly C, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
10.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flammand M, 2002. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in blood during the acute phase of disease in patient experiencing primary or secondary infection. J Clin Microbiol 40: 376–381.
-
11.
Shu P, Huang J, 2004. Current advances in dengue diagnosis. Clin Diagn Lab Immunol 11: 642–650.
-
12.
Gubler DJ, 1996. Serological diagnosis of dengue haemorrhagic fever. Dengue Bull 20: 20–23.
-
13.
Innis B, 1997. Antibody responses to dengue virus infections. Gubler DJ, Kuno G, eds. Dengue and Dengue Haemorrhagic Fever. New York: CAB International, 221–243.
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14.
World Medical Association Declaration of Helsinki, 2000. Ethical principles for medical research involving human subjects. JAMA 284: 3043–3045.
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15.
Kuberski TT, Rosen L, 1977. A simple technique for detection of dengue antigen in mosquitoes by immunofluorescence. Am J Trop Med Hyg 26: 533–537.
-
16.
Gubler DJ, Kuno G, Sather GE, Velez M, Oliver A, 1984. Use mosquitoes cell culture and specific monoclonal antibodies for routine surveillance of dengue viruses. Am J Trop Med Hyg 33: 158–165.
-
17.
Clarke DH, Cassals J, 1958. Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7: 561–573.
-
18.
World Health Organization, 1997. Management of dengue epidemic. Regional Guidelines on Dengue/DHF Prevention & Control. WHO/SEARO.
-
19.
Lam SK, Devi S, Pang T, 1987. Detection of specific IgM in dengue infection. Southeast Asian J Trop Med Public Health 18: 532–538.
-
20.
Yong YK, Thayan R, Chong HT, Tan CT, Sekaran SD, 2007. Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med J 448: 662–668.
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21.
Vajpayee M, Singh UB, Seth P, Broor S, 2001. Comparative evaluation of various commercial assays for diagnosis of dengue fever. Southeast Asian J Trop Med Health 32: 472–475.
-
22.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human. PLoS Negl Trop Dis 2: e280.
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23.
Lapphra K, Sangsharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T, Yoksan S, Wattanamongkolsil L, Thamlikitkul V, 2008. Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis 60: 387–391.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2385 | 1022 | 46 |
| Full Text Views | 1966 | 38 | 12 |
| PDF Downloads | 1034 | 20 | 0 |
Early Diagnosis of Dengue Infection Using a Commercial Dengue Duo Rapid Test Kit for the Detection of NS1, IGM, and IGG
Seok Mui Wang
Seok Mui Wang
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
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Shamala Devi Sekaran
Shamala Devi Sekaran
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
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A commercial Dengue Duo rapid test kit was evaluated for early dengue diagnosis by detection of dengue virus NS1 antigen and immunoglobulin M (IgM)/IgG antibodies. A total of 420 patient serum samples were subjected to real-time reverse transcription-polymerase chain reaction (RT-PCR), in-house IgM capture enzyme-linked immunosorbent assay (ELISA), hemagglutination inhibition assay, and the SD Dengue Duo rapid test. Of the 320 dengue acute and convalescent sera, dengue infection was detected by either serology or RT-PCR in 300 samples (93.75%), as compared with 289 samples (90.31%) in the combined SD Duo NS1/IgM. The NS1 detection rate is inversely proportional, whereas the IgM detection rate is directly proportional to the presence of IgG antibodies. The sensitivity and specificity in diagnosing acute dengue infection in the SD Duo NS1/IgM were 88.65% and 98.75%, respectively. The assay is sensitive and highly specific. Detection of both NS1 and IgM by SD Duo gave comparable detection rate by either serology or RT-PCR.
Author Notes
Authors' addresses: Seok Mui Wang and Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, E-mails: seokmuiwang@yahoo.com and shamalaya@yahoo.com.
Reprint requests: Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia, Tel: 603-79675755, Fax: 603-79676672, E-mail: shamalaya@yahoo.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Gubler DJ, Meltzer M, 1999. Impact of dengue/dengue hemorrhagic fever on the developing world. Adv Virus Res 53: 35–70.
-
2.
World Health Organization, 2000. Dengue haemorrhagic fever: diagnosis, treatment and control. Handbook of the World Health Organization. Geneva: WHO, 1–84.
-
3.
Innis BL, 1995. Dengue and dengue haemorrhagic fever. Porterfield JS, ed. Exotic Viral Infections. London: Chapman & Hall Medical, 103–140.
-
4.
Blacksell SD, Mammen MP, Thongpaseuth S, Gibbons RV, Jarman RG, Jenjaroen K, Nisalak A, Phetsouvanh R, Newton PN, Day NP, 2008. Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Diagn Microbiol Infect Dis 60: 43–49.
-
5.
Dussart P, Labeau B, Lagathu G, Louis P, Nunes MR, Rodrigues SG, Storck-Hermann C, Cesaire R, Morvan J, Flamand M, Baril L, 2006. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol 13: 1185–1189.
-
6.
Kumarasamy V, Wahab AH, Chua SK, Hassan Z, Chem YK, Mohamad M, Chua KB, 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. J Virol Methods 140: 75–79.
-
7.
McBride W, 2009. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis 64: 31–36.
-
8.
Sekaran SD, Lan EC, Mahesawarappa KB, Appanna R, Subramaniam G, 2007. Evaluation of a dengue NS1 capture ELISA assay for the rapid detection of dengue. J Infect Dev Ctries 1: 182–188.
-
9.
Young PR, Hilditch PA, Bletchly C, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
10.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flammand M, 2002. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in blood during the acute phase of disease in patient experiencing primary or secondary infection. J Clin Microbiol 40: 376–381.
-
11.
Shu P, Huang J, 2004. Current advances in dengue diagnosis. Clin Diagn Lab Immunol 11: 642–650.
-
12.
Gubler DJ, 1996. Serological diagnosis of dengue haemorrhagic fever. Dengue Bull 20: 20–23.
-
13.
Innis B, 1997. Antibody responses to dengue virus infections. Gubler DJ, Kuno G, eds. Dengue and Dengue Haemorrhagic Fever. New York: CAB International, 221–243.
-
14.
World Medical Association Declaration of Helsinki, 2000. Ethical principles for medical research involving human subjects. JAMA 284: 3043–3045.
-
15.
Kuberski TT, Rosen L, 1977. A simple technique for detection of dengue antigen in mosquitoes by immunofluorescence. Am J Trop Med Hyg 26: 533–537.
-
16.
Gubler DJ, Kuno G, Sather GE, Velez M, Oliver A, 1984. Use mosquitoes cell culture and specific monoclonal antibodies for routine surveillance of dengue viruses. Am J Trop Med Hyg 33: 158–165.
-
17.
Clarke DH, Cassals J, 1958. Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7: 561–573.
-
18.
World Health Organization, 1997. Management of dengue epidemic. Regional Guidelines on Dengue/DHF Prevention & Control. WHO/SEARO.
-
19.
Lam SK, Devi S, Pang T, 1987. Detection of specific IgM in dengue infection. Southeast Asian J Trop Med Public Health 18: 532–538.
-
20.
Yong YK, Thayan R, Chong HT, Tan CT, Sekaran SD, 2007. Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med J 448: 662–668.
-
21.
Vajpayee M, Singh UB, Seth P, Broor S, 2001. Comparative evaluation of various commercial assays for diagnosis of dengue fever. Southeast Asian J Trop Med Health 32: 472–475.
-
22.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human. PLoS Negl Trop Dis 2: e280.
-
23.
Lapphra K, Sangsharaswichai A, Chokephaibulkit K, Tiengrim S, Piriyakarnsakul W, Chakorn T, Yoksan S, Wattanamongkolsil L, Thamlikitkul V, 2008. Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. Diagn Microbiol Infect Dis 60: 387–391.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2385 | 1022 | 46 |
| Full Text Views | 1966 | 38 | 12 |
| PDF Downloads | 1034 | 20 | 0 |