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1.
Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lages-Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm NR, Tibayrenc M, Schijman AG, 2009. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem Inst Oswaldo Cruz 104: 1051–1054.
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2.
Tibayrenc M, 1998. Genetic epidemiology of parasitic protozoa and other infections agents: the need for an integrated approach. Int J Parasitol 28: 85–104.
-
3.
Coronado X, Zulantay I, Albrecht H, Rozas M, Apt W, Ortiz S, Rodriguez J, Sanchez G, Solari A, 2006. Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile. Am J Trop Med Hyg 74: 1008–1012.
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4.
Rozas M, Botto-Mahan C, Coronado X, Ortiz S, Cattan PE, Solari A, 2007. Coexistence of Trypanosoma cruzi genotypes in wild and peridomestic mammals in Chile. Am J Trop Med Hyg 77: 647–653.
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5.
Coronado X, Rozas M, Botto-Maham C, Ortiz S, Cattan P, Solari A, 2009. Molecular epidemiology of Chagas disease in the wild transmission cycle: the evaluation in the sylvatic vector Mepraia spinolai from an endemic area of Chile. Am J Trop Med Hyg 81: 656–659.
-
6.
Dias E, 1940. Xenodiagnosticos seriados em caes infectados con amostras venezuelanas de Schizotrypanum cruzi. Bras Med 54: 859–861.
-
7.
Schenone H, Contreras MC, Rojas A, 1991. Yielding of xenodiagnosis, according to the number of boxes used in 1,181 persons with chronic chagasic infection diagnosed with indirect hemagglutination reaction. Bol Chil Parasitol 46: 58–61.
-
8.
Westenberger SJ, Barnabé C, Campbell DA, Sturm NR, 2005. Two hybridization events define the population structure of Trypanosoma cruzi. Genetics 171: 527–543.
-
9.
Campos R, Acuña-Retamar M, Botto-Mahan C, Ortiz S, Cattan PE, Solari A, 2007. Susceptibility of Mepraia spinolai and Triatoma infestans to different Trypanosoma cruzi strains from naturally infected rodent hosts. Acta Trop 104: 25–29.
-
10.
Veas F, Breniere SF, Cuny G, Brengues C, Solari A, Tibayrenc M, 1991. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol Biol 37: 73–84.
-
11.
Lane JE, Ribeiro-Rodriguez R, Olivares-Villagomez D, Vnencak-Jones CL, McCurley T, Carter CE, 2003. Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction. Mem Inst Oswaldo Cruz 98: 373–376.
-
12.
de Lana M, da Silveira Pinto A, Barnabe C, Quesney V, Noel S, Tibayrenc M, 1998. Trypanosoma cruzi: compared vectorial transmissibility of three major clonal genotypes by Triatoma infestans. Exp Parasitol 90: 20–25.
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13.
Vago AR, Andrade LO, Leite AA, d`Avila Reis D, Macedo AM, Adad SJ, Tostes S Jr, Moreira MC, Filho GB, Pena SD, 2000. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. Am J Pathol 156: 1805–1809.
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Temporal Fluctuation of Infection with Different Trypanosoma cruzi Genotypes in the Wild Rodent Octodon degus
Ricardo Campos
Ricardo Campos
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
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Carezza Botto-Mahan
Carezza Botto-Mahan
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
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Sylvia Ortiz
Sylvia Ortiz
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
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Ximena Coronado
Ximena Coronado
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
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Aldo Solari
Aldo Solari
Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile
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We identified and followed-up for two years Octodon degus rodents infected with Trypanosoma cruzi genotypes by using xenodiagnosis with two vector species (Mepraia spinolai and Triatoma infestans), polymerase chain reaction DNA-based detection of insect dejections, Southern blot analysis, and minicircle hybridization with genotype-specific probes. Results show temporal fluctuations of infection with four parasite lineages (TCI, TCII, TCV, and TCVI) in one co-infected O. degus. Results are discussed in the context of parasitemia level and infection control in mammal hosts.
Author Notes
Financial support: This study was supported by Fondo Nacional de Desarrollo Científico y Tecnológico 1085154 (A. Solari), Comisión Nacional de Investigación Científica y Tecnológica–Programa Bicentenario de Ciencia y Tecnología PSD66, and Fondo Nacional de Desarrollo Científico y Tecnológico 11090086 (C. Botto-Mahan).
Disclosure: The authors have no conflicts of interest in relation to this work.
Authors' addresses: Ricardo Campos, Sylvia Ortiz, Ximena Coronado, and Aldo Solari, Program of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Casilla, Santiago, Chile, E-mail: asolari@machi.med.uchile.cl. Carezza Botto-Mahan and Sylvia Ortiz, Department of Ecological Sciences, Faculty of Sciences, University of Chile, Casilla, Santiago, Chile, E-mail: cbotto@uchile.cl.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lages-Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm NR, Tibayrenc M, Schijman AG, 2009. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem Inst Oswaldo Cruz 104: 1051–1054.
-
2.
Tibayrenc M, 1998. Genetic epidemiology of parasitic protozoa and other infections agents: the need for an integrated approach. Int J Parasitol 28: 85–104.
-
3.
Coronado X, Zulantay I, Albrecht H, Rozas M, Apt W, Ortiz S, Rodriguez J, Sanchez G, Solari A, 2006. Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile. Am J Trop Med Hyg 74: 1008–1012.
-
4.
Rozas M, Botto-Mahan C, Coronado X, Ortiz S, Cattan PE, Solari A, 2007. Coexistence of Trypanosoma cruzi genotypes in wild and peridomestic mammals in Chile. Am J Trop Med Hyg 77: 647–653.
-
5.
Coronado X, Rozas M, Botto-Maham C, Ortiz S, Cattan P, Solari A, 2009. Molecular epidemiology of Chagas disease in the wild transmission cycle: the evaluation in the sylvatic vector Mepraia spinolai from an endemic area of Chile. Am J Trop Med Hyg 81: 656–659.
-
6.
Dias E, 1940. Xenodiagnosticos seriados em caes infectados con amostras venezuelanas de Schizotrypanum cruzi. Bras Med 54: 859–861.
-
7.
Schenone H, Contreras MC, Rojas A, 1991. Yielding of xenodiagnosis, according to the number of boxes used in 1,181 persons with chronic chagasic infection diagnosed with indirect hemagglutination reaction. Bol Chil Parasitol 46: 58–61.
-
8.
Westenberger SJ, Barnabé C, Campbell DA, Sturm NR, 2005. Two hybridization events define the population structure of Trypanosoma cruzi. Genetics 171: 527–543.
-
9.
Campos R, Acuña-Retamar M, Botto-Mahan C, Ortiz S, Cattan PE, Solari A, 2007. Susceptibility of Mepraia spinolai and Triatoma infestans to different Trypanosoma cruzi strains from naturally infected rodent hosts. Acta Trop 104: 25–29.
-
10.
Veas F, Breniere SF, Cuny G, Brengues C, Solari A, Tibayrenc M, 1991. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol Biol 37: 73–84.
-
11.
Lane JE, Ribeiro-Rodriguez R, Olivares-Villagomez D, Vnencak-Jones CL, McCurley T, Carter CE, 2003. Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction. Mem Inst Oswaldo Cruz 98: 373–376.
-
12.
de Lana M, da Silveira Pinto A, Barnabe C, Quesney V, Noel S, Tibayrenc M, 1998. Trypanosoma cruzi: compared vectorial transmissibility of three major clonal genotypes by Triatoma infestans. Exp Parasitol 90: 20–25.
-
13.
Vago AR, Andrade LO, Leite AA, d`Avila Reis D, Macedo AM, Adad SJ, Tostes S Jr, Moreira MC, Filho GB, Pena SD, 2000. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. Am J Pathol 156: 1805–1809.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 30 | 30 | 7 |
| Full Text Views | 209 | 81 | 0 |
| PDF Downloads | 55 | 32 | 0 |