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Temporal Fluctuation of Infection with Different Trypanosoma cruzi Genotypes in the Wild Rodent Octodon degus

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  • Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, e Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

We identified and followed-up for two years Octodon degus rodents infected with Trypanosoma cruzi genotypes by using xenodiagnosis with two vector species (Mepraia spinolai and Triatoma infestans), polymerase chain reaction DNA-based detection of insect dejections, Southern blot analysis, and minicircle hybridization with genotype-specific probes. Results show temporal fluctuations of infection with four parasite lineages (TCI, TCII, TCV, and TCVI) in one co-infected O. degus. Results are discussed in the context of parasitemia level and infection control in mammal hosts.

Author Notes

*Address correspondence to Aldo Solari, Program of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Casilla 70086, Santiago 7, Chile. E-mail: asolari@machi.med.uchile.cl

Financial support: This study was supported by Fondo Nacional de Desarrollo Científico y Tecnológico 1085154 (A. Solari), Comisión Nacional de Investigación Científica y Tecnológica–Programa Bicentenario de Ciencia y Tecnología PSD66, and Fondo Nacional de Desarrollo Científico y Tecnológico 11090086 (C. Botto-Mahan).

Disclosure: The authors have no conflicts of interest in relation to this work.

Authors' addresses: Ricardo Campos, Sylvia Ortiz, Ximena Coronado, and Aldo Solari, Program of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Casilla, Santiago, Chile, E-mail: asolari@machi.med.uchile.cl. Carezza Botto-Mahan and Sylvia Ortiz, Department of Ecological Sciences, Faculty of Sciences, University of Chile, Casilla, Santiago, Chile, E-mail: cbotto@uchile.cl.

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