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Molecular Evidence of Bartonella Infection in Domestic Dogs from Algeria, North Africa, by Polymerase Chain Reaction (PCR)

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  • Département de zoologie, Institut National d'Agronomie, El Harrach, Algiers, Algeria; École Nationale Vétérinaire d'El Harrach, Algiers, Algeria; Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California; Unité de recherche en maladies infectieuses tropicales émergentes, URMITE CNRS-IRD, Faculté de Médecine, Université de la Méditerranée, Marseille, France; Service d'Ecologie des Systèmes Vectoriels, Institut Pasteur d'Algérie, Algiers, Algeria

Bartonella species are being recognized as important bacterial human and canine pathogens, and are associated with multiple arthropod vectors. Bartonella DNA extracted from blood samples was obtained from domestic dogs in Algiers, Algeria. Polymerase chain reaction (PCR) and DNA sequence analyses of the ftsZ gene and the 16S-23S intergenic spacer region (ITS) were performed. Three Bartonella species: Bartonella vinsonii subsp. berkhoffii, Bartonella clarridgeiae, and Bartonells elizabethae were detected infecting Algerian dogs. To our knowledge, this study is the first report of detection by PCR amplification of Bartonella in dogs in North Africa.

Author Notes

*Address correspondence to Didier Raoult, Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385 Marseille, Cedex 5, France. E-mail: didier.raoult@medecine.univ-mrs.fr

Authors' addresses: Tahar Kernif, Department de Zoologie, Institut National d'Agronomie, Algiers, Algeria and Service d'Ecologie des Sysytèmes Vectoriels, Institut Pasteur d'Alger, Annexe Ruisseau, Algiers, Algeria, E-mail: taharscorse@hotmail.com. Meriem Aissi, École Nationale Vétérinaire d'El Harrach, Algiers, Algeria, E-mail: aissimiriem@yahoo.fr. Salah-Eddine Doumandji, Department de Zoologie, Institut National d'Agronomie, Algiers, Algeria, E-mail: dmndjislhdn@yahoo.fr. Bruno B. Chomel, Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA, E-mail: bbchomel@ucdavis.edu. Didier Raoult, Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Université de la Méditerranée, Marseille, Cedex 5, France, E-mail: didier.Raoult@medecine.univ-mrs.fr. Idir Bitam, Service d'Ecologie des Systèmes Vectoriels, Institut Pasteur d'Alger, Annexe Ruisseau, Algiers, Algeria, E-mail: idirbitam@yahoo.fr.

Reprint requests: Idir Bitam, Service d'Ecologie des Systèmes Vectoriels, Institut Pasteur d'Alger, Annexe Ruisseau, Algiers, Algeria, E-mail: idirbitam@yahoo.fr.

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