Indoor Use of Plastic Sheeting Impregnated with Carbamate Combined with Long-Lasting Insecticidal Mosquito Nets for the Control of Pyrethroid-Resistant Malaria Vectors

Armel Djènontin Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Fabrice Chandre Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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K. Roch Dabiré Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Joseph Chabi Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Raphael N'Guessan Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Thierry Baldet Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Martin Akogbéto Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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Vincent Corbel Centre de Recherche Entomologique de Cotonou, Unité de Recherche 016 Institut de Recherche pour le Développement, Cotonou, Bénin; Institut de Recherche en Science de la Santé, Centre Muraz, Bobo Dioulasso, Burkina Faso; Institut de Recherche pour le Développement, Laboratoire de Lutte contre les Insectes Nuisibles, Montpellier, France

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The combined efficacy of a long-lasting insecticidal net (LLIN) and a carbamate-treated plastic sheeting (CTPS) or indoor residual spraying (IRS) for control of insecticide-resistant mosquitoes was evaluated in experimental huts in Burkina Faso. Anopheles gambiae from the area is resistant to pyrethroids and to a lesser extent, carbamates. Relatively low mortality rates were observed with the LLIN (44%), IRS (42%), and CTPS (52%), whereas both combinations killed significantly more mosquitoes (~70% for LLIN + CTPS and LLIN + IRS). Blood feeding by An. gambiae was uninhibited by IRS and CTPS compared with LLIN (43%), LLIN + CTPS (58%), and LLIN + IRS (56%). No evidence for selection of the kdr and ace-1R alleles was observed with the combinations, whereas a survival advantage of mosquitoes bearing the ace-1R mutation was observed with IRS and CTPS. The results suggest that the combination of the two interventions constitutes a potential tool for vector-resistance management.

Author Notes

*Address correspondence to Armel Djènontin, Institut de Recherche pour le Développement (IRD)/Centre de Recherche Entomologique de Cotonou (CREC), 01 BP 4414 RP Cotonou, République du Bénin. E-mail: armeldj@yahoo.fr

Authors' addresses: Armel Djènontin, Joseph Chabi, Thierry Baldet, and Vincent Corbel, Institut de Recherche pour le Développement (IRD)/Centre de Recherche Entomologique de Cotonou (CREC), Cotonou, République du Bénin, E-mails: armeldj@yahoo.fr, joseph.chabi@ird.fr, thierry.baldet@ird.fr, and vincent.corbel@ird.fr. Fabrice Chandre, Institut de Recherche pour le Développement (IRD)/Laboratoire de lutte contre les Insectes Nuisibles (LIN), Centre Collaborateur, Montpellier Cedex 5, France, E-mail: fabrice.chandre@ird.fr. K. Roch Dabiré, Institut de Recherche en Sciences de la Santé (IRSS)/Centre Muraz (CM), Bobo-Dioulasso, Burkina Faso, E-mail: dabire_roch@hotmail.com. Raphael N'Guessan, London School of Hygiene and Tropical Medicine (LSHTM)/Centre de Recherche Entomologique de Cotonou (CREC), Cotonou, République du Bénin, E-mail: Raphael.N'Guessan@lshtm.ac.uk. Martin Akogbéto, Centre de Recherche Entomologique de Cotonou (CREC), Cotonou, République du Bénin, E-mail: akogbetom@yahoo.fr.

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