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Reference Manager
BibTeX
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-
1.
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383–416.
-
2.
Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159: 890–899.
-
4.
Sirisinha S, Anuntagool N, Dharakul T, Ekpo P, Wongratanacheewin S, Naigowit P, Petchclai B, Thamlikitkul V, Suputtamongkol Y, 2000. Recent developments in laboratory diagnosis of melioidosis. Acta Trop 74: 235–245.
-
5.
Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T, 2000. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74: 247–251.
-
6.
Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49: 1075–1078.
-
7.
Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S, 2000. Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63: 146–149.
-
8.
Reckseidler-Zenteno SL, DeVinney R, Woods DE, 2005. The capsular polysaccharide of Burkholderia pseudomallei contributes to survival in serum by reducing complement factor C3b deposition. Infect Immun 73: 1106–1115.
-
9.
Rattanathongkom A, Sermswan RW, Wongratanacheewin S, 1997. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11: 25–31.
-
10.
Sambrook J, Russell DW, 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
-
11.
Kim HS, Schell MA, Yu Y, Ulrich RL, Sarria SH, Nierman WC, DeShazer D, 2005. Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies. BMC Genomics 6: 174.
-
12.
Wongratanacheewin S, Kespichayawattana W, Intachote P, Pichyangkul S, Sermswan RW, Krieg AM, Sirisinha S, 2004. Immunostimulatory CpG oligodeoxynucleotide confers protection in a murine model of infection with Burkholderia pseudomallei. Infect Immun 72: 4494–4502.
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Bacterial Loads and Antibody Responses in BALB/c Mice Infected with Low and High Doses of Burkholderia pseudomallei
Nuttiya Srisurat
Nuttiya Srisurat
Department of Microbiology, Department of Biochemistry, and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
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Rasana W. Sermswan
Rasana W. Sermswan
Department of Microbiology, Department of Biochemistry, and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
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Unchalee Tatawasart
Unchalee Tatawasart
Department of Microbiology, Department of Biochemistry, and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
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Surasakdi Wongratanacheewin
Surasakdi Wongratanacheewin
Department of Microbiology, Department of Biochemistry, and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
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Profiles of Burkholderia pseudomallei persistence and antibodies in blood, spleen, liver, and lungs of infected BALB/c mice were investigated. Animals were infected intraperitoneally with low (6 colony-forming units) or high (230 colony-forming units) doses of B. pseudomallei. In the high-dose infected group, bacteria were found by culture in 100% of blood, liver, spleen and lung samples at 24 and 48 hours after infections; blood samples were 100% positive by polymerase chain reaction after 60 hours. Antibody responses in the high-dose infected group were low. These responses were detected in the low-dose infected group after 5 days, peaked at 7–14 days, and showed persistence until 28 days post-infection. Bacterial loads and antibody profiles varied according to the level of bacterial infections. This kinetic study, although in animals, provides crucial knowledge that might be useful for the development of a sensitive and specific diagnostic assay for patients with melioidosis.
Author Notes
Financial support: This study was supported by the Commission on Higher Education, Thailand (project CHE-RG), and Melioidosis Research Center of Khon Kaen University.
Authors' addresses: Nuttiya Srisurat, Unchalee Tatawasart, and Surasakdi Wongratanacheewin, Department of Microbiology and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: nsrisurat@yahoo.com, unchalee@kku.ac.th, and sura_wng@kku.ac.th. Rasana W. Sermswan, Department of Biochemistry and Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: rasana@kku.ac.th.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383–416.
-
2.
Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N, 1989. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159: 890–899.
-
4.
Sirisinha S, Anuntagool N, Dharakul T, Ekpo P, Wongratanacheewin S, Naigowit P, Petchclai B, Thamlikitkul V, Suputtamongkol Y, 2000. Recent developments in laboratory diagnosis of melioidosis. Acta Trop 74: 235–245.
-
5.
Kunakorn M, Raksakait K, Sethaudom C, Sermswan RW, Dharakul T, 2000. Comparison of three PCR primer sets for diagnosis of septicemic melioidosis. Acta Trop 74: 247–251.
-
6.
Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49: 1075–1078.
-
7.
Sermswan RW, Wongratanacheewin S, Anuntagool N, Sirisinha S, 2000. Comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. Am J Trop Med Hyg 63: 146–149.
-
8.
Reckseidler-Zenteno SL, DeVinney R, Woods DE, 2005. The capsular polysaccharide of Burkholderia pseudomallei contributes to survival in serum by reducing complement factor C3b deposition. Infect Immun 73: 1106–1115.
-
9.
Rattanathongkom A, Sermswan RW, Wongratanacheewin S, 1997. Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction. Mol Cell Probes 11: 25–31.
-
10.
Sambrook J, Russell DW, 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
-
11.
Kim HS, Schell MA, Yu Y, Ulrich RL, Sarria SH, Nierman WC, DeShazer D, 2005. Bacterial genome adaptation to niches: divergence of the potential virulence genes in three Burkholderia species of different survival strategies. BMC Genomics 6: 174.
-
12.
Wongratanacheewin S, Kespichayawattana W, Intachote P, Pichyangkul S, Sermswan RW, Krieg AM, Sirisinha S, 2004. Immunostimulatory CpG oligodeoxynucleotide confers protection in a murine model of infection with Burkholderia pseudomallei. Infect Immun 72: 4494–4502.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 302 | 261 | 10 |
| Full Text Views | 339 | 8 | 0 |
| PDF Downloads | 78 | 9 | 0 |