• 1.

    Dias E, Laranja FS, Miranda A, Nobrega G, 1956. Chagas' disease: a clinical, epidemiologic, and pathologic study. Circulation 14: 10351060.

  • 2.

    World Health Organization, 2002. Control of Chagas disease: report of a WHO expert committee. World Health Organ Tech Rep Ser 905: 1109.

    • Search Google Scholar
    • Export Citation
  • 3.

    Tarleton RL, 2007. Immune system recognition of Trypanosoma cruzi. Curr Opin Immunol 19: 430434.

  • 4.

    Parish IA, Kaech SM, 2009. Diversity in CD8+ T cell differentiation. Curr Opin Immunol 21: 291297.

  • 5.

    Callan MF, Fazou C, Yang H, Rostron T, Poon K, Hatton C, McMichael AJ, 2000. CD8+ T cell selection, function, and death in the primary immune response in vivo. J Clin Invest 106: 12511261.

    • Search Google Scholar
    • Export Citation
  • 6.

    Harari A, Rizzardi GP, Ellefsen K, Ciuffreda D, Champagne P, Bart PA, Kaufmann D, Telenti A, Sahli R, Tambussi G, Kaiser L, Lazzarin A, Perrin L, Pantaleo G, 2002. Analysis of HIV-1 and CMV-specific memory CD4 T cell responses during primary and chronic infection. Blood 100: 13811387.

    • Search Google Scholar
    • Export Citation
  • 7.

    Amyes E, Hatton C, Montamat-Sicotte D, Gudgeon N, Rickinson AB, McMichael AJ, Callan MF, 2003. Characterization of the CD4+ T cell response to Epstein-Barr virus during primary and persistent infection. J Exp Med 198: 903911.

    • Search Google Scholar
    • Export Citation
  • 8.

    Papagno L, Spina CA, Marchant A, Salio M, Rufer N, Little S, Dong T, Chesney G, Waters A, Easterbrook P, Dunbar PR, Shepherd D, Cerundolo V, Emery V, Griffiths P, Conlon C, McMichael AJ, Richman DD, Rowland-Jones SL, Appay V, 2004. Immune activation and CD8+ T-cell differentiation towards senescence in HIV-1 infection. PLoS Biol 2: E20.

    • Search Google Scholar
    • Export Citation
  • 9.

    Nikolich-Zugich J, 2008. Ageing and life-long maintenance of T-cell subsets in the face of latent persistent infections. Nat Rev Immunol 8: 512522.

    • Search Google Scholar
    • Export Citation
  • 10.

    Laucella SA, Postan M, Martin D, Hubby-Fralish B, Albareda MC, Alvarez MG, Lococo B, Barbieri G, Viotti R, Tarleton RL, 2004. The frequency of IFN-γ-producing T cells specific for Trypanosoma cruzi inversely correlates with disease severity in chronic human Chagas disease. J Infect Dis 189: 909918.

    • Search Google Scholar
    • Export Citation
  • 11.

    Albareda MC, Laucella SA, Alvarez MG, Armenti AH, Bertochi G, Tarleton RL, Postan M, 2006. Trypanosoma cruzi modulates the profile of memory CD8+ T cells in chronic Chagas disease patients. Int Immunol 18: 465471.

    • Search Google Scholar
    • Export Citation
  • 12.

    Alvarez MG, Postan M, Weatherly B, Albareda MC, Sidney J, Sette A, Olivera C, Armenti AH, Tarleton RL, Laucella S, 2008. HLA Class I-T cell epitopes from trans-sialidase proteins reveal functionally distinct subsets of CD8+ T cells specific for Trypanosoma cruzi in chronic Chagas disease. PLoS Negl Trop Dis 2: e288.

    • Search Google Scholar
    • Export Citation
  • 13.

    Albareda MC, Olivera GC, Laucella SA, Alvarez MG, Fernandez ER, Lococo B, Viotti R, Tarleton RL, Postan M, 2009. Chronic human infection with Trypanosoma cruzi drives CD4+ T cells to immune senescence. J Immunol 183: 41034108.

    • Search Google Scholar
    • Export Citation
  • 14.

    Seki Y, Yang J, Okamoto M, Tanaka S, Goitsuka R, Farrar MA, Kubo M, 2007. IL-7/STAT5 cytokine signaling pathway is essential but insufficient for maintenance of naive CD4 T cell survival in peripheral lymphoid organs. J Immunol 178: 262270.

    • Search Google Scholar
    • Export Citation
  • 15.

    Rabin RL, Roederer M, Maldonado Y, Petru A, Herzenberg LA, Herzenberg LA, 1995. Altered representation of naive and memory CD8 T cell subsets in HIV-infected children. J Clin Invest 95: 20542060.

    • Search Google Scholar
    • Export Citation
  • 16.

    Sathler-Avelar R, Vitelli-Avelar DM, Massara RL, Borges JD, Lana M, Teixeira-Carvalho A, Dias JC, Elói-Santos SM, Martins-Filho OA, 2006. Benznidazole treatment during early-indeterminate Chagas' disease shifted the cytokine expression by innate and adaptive immunity cells toward a type 1-modulated immune profile. Scand J Immunol 64: 554563.

    • Search Google Scholar
    • Export Citation
  • 17.

    Bahia-Oliveira LM, Gomes JA, Cancado JR, Ferrari TC, Lemos EM, Luz ZM, Moreira MC, Gazzinelli G, Correa-Oliveira R, 2000. Immunological and clinical evaluation of chagasic patients subjected to chemotherapy during the acute phase of Trypanosoma cruzi infection 14–30 years ago. J Infect Dis 182: 634638.

    • Search Google Scholar
    • Export Citation
  • 18.

    Bahia-Oliveira LM, Gomes JA, Rocha MO, Moreira MC, Lemos EM, Luz ZM, Pereira ME, Coffman RL, Dias JC, Cançado JR, Gazzinelli G, Corrêa-Oliveira R, 1998. IFN-gamma in human Chagas' disease: protection or pathology? Braz J Med Biol Res 31: 127131.

    • Search Google Scholar
    • Export Citation
  • 19.

    Laucella SA, Perez Mazliah D, Bertocchi G, Alvarez AM, Cooley G, Viotti R, Albareda MC, Lococo B, Postan M, Tarleton RL, 2009. Changes in Trypanosoma cruzi-specific immune responses following treatment: surrogate markers of treatment efficacy. CID 49: 16751684.

    • Search Google Scholar
    • Export Citation
  • 20.

    Sosa Estani S, Segura EL, Ruiz AM, Velazquez E, Porcel BM, Yampotis C, 1998. Efficacy of treatment with benznidazole in children. Am J Trop Med Hyg 59: 526529.

    • Search Google Scholar
    • Export Citation
  • 21.

    de Andrade AL, Zicker F, de Oliveira RM, Almeida Silva S, Luquetti A, Travassos LR, Almeida IC, de Andrade SS, de Andrade JG, Martelli CM, 1996. Randomized trial of efficacy of benznidazole in treatment of early Trypanosoma cruzi infection. Lancet 9039: 14071413.

    • Search Google Scholar
    • Export Citation
  • 22.

    Viotti R, Vigliano C, Lococo B, Bertocchi G, Petti M, Alvarez MG, Postan M, Armenti A, 2006. Long-term cardiac outcomes of treating chronic Chagas disease with benznidazole versus no treatment: a nonrandomized trial. Ann Intern Med 144: 724734.

    • Search Google Scholar
    • Export Citation
  • 23.

    Rassi A Jr, Dias JC, Marin-Neto JA, Rassi A, 2009. Challenges and opportunities for primary, secondary, and tertiary prevention of Chagas' disease. Heart 95: 524534.

    • Search Google Scholar
    • Export Citation
  • 24.

    Laucella S, Segura E, Riarte A, Sosa Estani S, 1999. Soluble platelet (sP-selectin) and soluble vascular cell adhesion molecule-1 (sVCAM-1) decrease during therapy with benznidazole in children with indeterminate form of Chagas' disease. Clin Exp Immunol 118: 423427.

    • Search Google Scholar
    • Export Citation
  • 25.

    Sathler-Avelar R, Vitelli-Avelar DM, Massara RL, de Lana M, Pinto Dias JC, Teixeira-Carvalho A, Elói-Santos SM, Martins-Filho OA, 2008. Etiological treatment during early chronic indeterminate Chagas disease incites an activated status on innate and adaptive immunity associated with a type 1-modulated cytokine pattern. Microbes Infect 10: 103113.

    • Search Google Scholar
    • Export Citation
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Assessment of CD8+ T Cell Differentiation in Trypanosoma cruzi-Infected Children

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  • Instituto Nacional de Parasitología “Dr. M. Fatala Chaben,” Ciudad Autónoma de Buenos Aires, Argentina
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We previously reported that the T cell compartment in chronically Trypanosoma cruzi-infected adult subjects display functional and phenotypic signs of immune senescence. This study aimed to investigate the differentiation and the senescent profile of the overall CD8+ T cell compartment in T. cruzi-infected children at the early stage of the disease. We found a lower percentage of naive (CD27+CD28+CD45RA+) and early antigen-experienced (CD45RACD27+CD28+), and higher percentages of late differentiated antigen-experienced (CD45RACD27CD28) CD8+ T cells in T. cruzi-infected children as compared with age-matched uninfected controls. The expression of the interleukin (IL)-7R is also decreased on naive and on antigen-experienced total CD8+ T cells with various degrees of differentiation. Conversely, the expression of HLA-DR, caspase-3, and CD57 did not vary on the total CD8+ T cell compartment. These findings suggest that the duration of the infection is relevant in the process of immune senescent that this parasite can induce.

Author Notes

*Address correspondence to María Cecilia Albareda, Instituto Nacional de Parasitología, “Dr M Fatala Chaben,” Avda. Paseo Colón 568, 1063, Ciudad Autónoma de Buenos Aires, Argentina. E-mail: mcalbareda@gmail.com

Financial support: This work was supported by Bunge & Born Foundation, Buenos Aires Argentina and Ministerio de Salud, Buenos Aires, Argentina.

Authors' addresses: M. Cecilia Albareda, Gabriela C. Olivera, Ana M. De Rissio, and Miriam Postan, Instituto Nacional de Parasitología, “Dr M Fatala Chaben,” Ciudad Autónoma de Buenos Aires, Argentina, E-mails: mcalbareda@gmail.com, oliveragc@gmail.com, amderissio@yahoo.com.ar, and miriampostan@yahoo.com.

Reprint Requests: M. Cecilia Albareda, Instituto Nacional de Parasitología, “Dr M Fatala Chaben,” Avda. Paseo Colón 568, ZIP code 1063, Ciudad de Buenos Aires, Argentina, E-mail: mcalbareda@gmail.com.

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