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Heterogeneity of Leishmania infantum chagasi Kinetoplast DNA in Teresina (Brazil)

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  • Department of Parasitology, Bioscience Institute, Sao Paulo State University, Botucatu, Brazil (UNESP); Laboratory for Research in Leishmaniasis, Natan Portella Institute for Tropical Diseases, Teresina, Brazil; Laboratory for Animal Health, Federal University of Piauí, Teresina, Brazil
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Leishmania infantum chagasi is the causative agent of visceral leishmaniasis in Brazil. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of kinetoplast DNA (kDNA) minicircles was used to evaluate genetic profiles of 48 Leishmania infantum chagasi strains from dog and human parasite cultures, fresh collected dog bone marrow aspirates, and from infected sand flies. Results revealed that heterogeneity in kDNA minicircles depends mostly on the source of the samples, with cultured parasites showing a high degree of homogeneity.

Author Notes

*Address correspondence to Paulo Eduardo Martins Ribolla, Rubião Junior sn, CP 510, 18618000 Botucatu, São Paulo, Brazil. E-mail: pribolla@ibb.unesp.br

Financial support: Sao Paulo State Funding Agency (FAPESP, 2006/61151-2).

Authors' addresses: Diego Peres Alonso and Paulo Eduardo Martins Ribolla, Parasitology Department, Bioscience Institute (UNESP), Rubiao Junior s/n, CP 510, CEP 18618-000, Botucatu, Brazil. Dorcas Lamounier Costa and Carlos Henrique Nery Costa, Instituto de Doencas Tropicais Natan Portella, Rua Arthur de Vasconcelos, 151-Sul, Teresina Piaui 64001-450, Brazil. Ivete Lopes de Mendonca, Laboratório de Sanidade Animal, Universidade Federal do Piauí, Campus Socopo, Teresina Piaui 64049-550, Brazil.

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