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Antibody Response from Whole-Cell Pertussis Vaccine Immunized Brazilian Children against Different Strains of Bordetella pertussis

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  • 1 Genetics, Virology, Biochemistry and Immunochemistry Laboratories, Butantan Institute, São Paulo, Brazil; College of Medicine, University of São Paulo, São Paulo, Brazil

Bordetella pertussis is a gram-negative bacillus that causes the highly contagious disease known as pertussis or whooping cough. Antibody response in children may vary depending on the vaccination schedule and the product used. In this study, we have analyzed the antibody response of cellular pertussis vaccinated children against B. pertussis strains and their virulence factors, such as pertussis toxin, pertactin, and filamentous hemagglutinin. After the completion of the immunization process, according to the Brazilian vaccination program, children serum samples were collected at different periods of time, and tested for the presence of specific antibodies and antigenic cross-reactivity. Results obtained show that children immunized with three doses of the Brazilian whole-cell pertussis vaccine present high levels of serum antibodies capable of recognizing the majority of the components present in vaccinal and non-vaccinal B. pertussis strains and their virulence factors for at least 2 years after the completion of the immunization procedure.

Author Notes

*Address correspondence to Denise V. Tambourgi, Immunochemistry Laboratory, Butantan Institute, Av. Prof. Vital Brazil, 1500, CEP 05503-900 São Paulo, Brazil. E-mail: dvtambourgi@butantan.gov.br

Financial support: This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo and Conselho Nacional de Desenvolvimento Científico e Tecnológico.

Authors' addresses: Alexandre Pereira, Genetics Laboratory, Butantan Institute, Av. Prof. Vital Brazil, São Paulo, SP, Brazil, E-mail: pereira_alex@butantan.gov.br. Aparecida S. Pietro Pereira, Virology Laboratory, Butantan Institute, Av. Prof. Vital Brazil, São Paulo, Brazil, E-mail: cidapietro@butantan.gov.br. Ivo Lebrun, Biochemistry Laboratory, Butantan Institute, Av. Prof. Vital Brazil, São Paulo, SP, Brazil, E-mail: lebrun@butantan.gov.br. Célio Lopes Silva, School of Medicine, University of São Paulo/Ribeirão Preto, Av. Bandeirantes, Ribeirão Preto, SP, Brazil, E-mail: clsilva@cpt.fmrp.usp.br. Gutemberg de Melo Rocha, School of Medicine, University of São Paulo/Ribeirã Preto, Av. Bandeirantes, Ribeirão Preto, SP, Brazil, E-mail: gdmrocha@fmrp.usp.br. Osvaldo A. Sant'Anna, Immunochemistry Laboratory, Butantan Institute, Av. Prof. Vital Brazil, São Paulo, SP, Brazil, E-mail: oasantanna@butantan.gov.br. Denise V. Tambourgi, Immunochemistry Laboratory, Butantan Institute, Av. Prof. Vital Brazil, São Paulo, Brazil, E-mail: dvtambourgi@butantan.gov.br.

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