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1.
Smilkstein M, Sriwilaijaroen N, Kelly JX, Wilairat P, Riscoe M, 2004. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrob Agents Chemother 48: 1803–1806.
-
2.
Bennett TN, Paguio M, Gligorijevic B, Seudieu C, Kosar AD, Davidson E, Roepe PD, 2004. Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy. Antimicrob Agents Chemother 48: 1807–1810.
-
3.
Baniecki ML, Wirth DF, Clardy J, 2007. High-throughput Plasmodium falciparum growth assay for malaria drug discovery. Antimicrob Agents Chemother 51: 716–723.
-
4.
Corbett Y, Herrera L, Gonzalez J, Cubilla L, Capson TL, Coley PD, Kursar TA, Romero LI, Ortega-Barria E, 2004. A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity. Am J Trop Med Hyg 70: 119–124.
-
5.
Sigma Aldrich. SYBR Green I product information. Available at: http://www.sigmaaldrich.com/sigma-aldrich/datasheet/s9430dat.pdf. Accessed July 17, 2008.
-
6.
Noedl H, Bronnert J, Yingyuen K, Attlmayr B, Kollaritsch H, Fukuda M, 2005. Simple histidine-rich protein 2 double-site sandwich enzyme-linked immunosorbent assay for use in malaria drug sensitivity testing. Antimicrob Agents Chemother 49: 3575–3577.
-
7.
Basco LK, Marquet F, Makler MM, Le Bras J, 1995. Plasmodium falciparum and Plasmodium vivax: lactate dehydrogenase activity and its application for in vitro drug susceptibility assay. Exp Parasitol 80: 260–271.
-
8.
Bacon DJ, Latour C, Lucas C, Colina O, Ringwald P, Picot S, 2007. Comparison of a SYBR green I-based assay with a histidine-rich protein II enzyme-linked immunosorbent assay for in vitro antimalarial drug efficacy testing and application to clinical isolates. Antimicrob Agents Chemother 51: 1172–1178.
-
9.
Noedl H, Krudsood S, Leowattana W, Tangpukdee N, Thanachartwet W, Looareesuwan S, Miller RS, Fukuda M, Jongsakul K, Yingyuen K, Sriwichai S, Ohrt C, Knirsch C, 2007. In vitro antimalarial activity of azithromycin, artesunate, and quinine in combination and correlation with clinical outcome. Antimicrob Agents Chemother 51: 651–656.
-
10.
Rason MA, Randriantsoa T, Andrianantenaina H, Ratsimbasoa A, Menard D, 2008. Performance and reliability of the SYBR Green I based assay for the routine monitoring of susceptibility of Plasmodium falciparum clinical isolates. Trans R Soc Trop Med Hyg 102:346–351.
-
11.
Desjardins RE, Canfield CJ, Haynes JD, Chulay JD, 1979. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 16: 710–718.
-
12.
Menard D, Djalle D, Manirakiza A, Yapou F, Siadoua V, Sana S, Matsika-Claquin MD, Nestor M, Talarmin A, 2005. Drug-resistant malaria in Bangui, Central African Republic: an in vitro assessment. Am J Trop Med Hyg 73: 239–243.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 836 | 438 | 145 |
| Full Text Views | 755 | 14 | 0 |
| PDF Downloads | 292 | 17 | 0 |
The SYBR Green I Malaria Drug Sensitivity Assay: Performance in Low Parasitemia Samples
Matthias G. Vossen
Matthias G. Vossen
Institute of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria; MARIB - Malaria Research Initiative Bandarban, New Gulshan, Bandarban 4600, Bangladesh; Institute of Medical Chemistry, Center for Physiology and Pathophysiology, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria
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Sandra Pferschy
Sandra Pferschy
Institute of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria; MARIB - Malaria Research Initiative Bandarban, New Gulshan, Bandarban 4600, Bangladesh; Institute of Medical Chemistry, Center for Physiology and Pathophysiology, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria
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Peter Chiba
Peter Chiba
Institute of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria; MARIB - Malaria Research Initiative Bandarban, New Gulshan, Bandarban 4600, Bangladesh; Institute of Medical Chemistry, Center for Physiology and Pathophysiology, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria
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Harald Noedl
Harald Noedl
Institute of Specific Prophylaxis and Tropical Medicine, Center for Physiology and Pathophysiology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria; MARIB - Malaria Research Initiative Bandarban, New Gulshan, Bandarban 4600, Bangladesh; Institute of Medical Chemistry, Center for Physiology and Pathophysiology, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria
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Validation of the sensitivity of the SYBR Green I in vitro test against an enzyme-linked immunosorbent assay (ELISA)-based drug sensitivity assay. Our results suggest that the SYBR Green I assay is a fast and inexpensive malaria drug screening assay for laboratory use. However, because of its lack of sensitivity in whole blood samples its usefulness for testing clinical samples may be limited.
Author Notes
Authors' addresses: Matthias G. Vossen and Harald Noedl, Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria, E-mails: matthias.vossen@meduniwien.ac.at and harald.noedl@meduniwien.ac.at. Sandra Pferschy, Department for Medical Genetics, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria, E-mail: sandra.pferschy@meduniwien.ac.at. Peter Chiba, Department of Medical Chemistry, Medical University of Vienna, Währingerstr. 10, A-1090 Vienna, Austria, E-mail: peter.chiba@meduniwien.ac.at.
Reprint requests: Harald Noedl, Department of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria, Tel: +43-1-4277-64882, Fax: +43-1-4277-64899, E-mail: harald.noedl@meduniwien.ac.at.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Smilkstein M, Sriwilaijaroen N, Kelly JX, Wilairat P, Riscoe M, 2004. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrob Agents Chemother 48: 1803–1806.
-
2.
Bennett TN, Paguio M, Gligorijevic B, Seudieu C, Kosar AD, Davidson E, Roepe PD, 2004. Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy. Antimicrob Agents Chemother 48: 1807–1810.
-
3.
Baniecki ML, Wirth DF, Clardy J, 2007. High-throughput Plasmodium falciparum growth assay for malaria drug discovery. Antimicrob Agents Chemother 51: 716–723.
-
4.
Corbett Y, Herrera L, Gonzalez J, Cubilla L, Capson TL, Coley PD, Kursar TA, Romero LI, Ortega-Barria E, 2004. A novel DNA-based microfluorimetric method to evaluate antimalarial drug activity. Am J Trop Med Hyg 70: 119–124.
-
5.
Sigma Aldrich. SYBR Green I product information. Available at: http://www.sigmaaldrich.com/sigma-aldrich/datasheet/s9430dat.pdf. Accessed July 17, 2008.
-
6.
Noedl H, Bronnert J, Yingyuen K, Attlmayr B, Kollaritsch H, Fukuda M, 2005. Simple histidine-rich protein 2 double-site sandwich enzyme-linked immunosorbent assay for use in malaria drug sensitivity testing. Antimicrob Agents Chemother 49: 3575–3577.
-
7.
Basco LK, Marquet F, Makler MM, Le Bras J, 1995. Plasmodium falciparum and Plasmodium vivax: lactate dehydrogenase activity and its application for in vitro drug susceptibility assay. Exp Parasitol 80: 260–271.
-
8.
Bacon DJ, Latour C, Lucas C, Colina O, Ringwald P, Picot S, 2007. Comparison of a SYBR green I-based assay with a histidine-rich protein II enzyme-linked immunosorbent assay for in vitro antimalarial drug efficacy testing and application to clinical isolates. Antimicrob Agents Chemother 51: 1172–1178.
-
9.
Noedl H, Krudsood S, Leowattana W, Tangpukdee N, Thanachartwet W, Looareesuwan S, Miller RS, Fukuda M, Jongsakul K, Yingyuen K, Sriwichai S, Ohrt C, Knirsch C, 2007. In vitro antimalarial activity of azithromycin, artesunate, and quinine in combination and correlation with clinical outcome. Antimicrob Agents Chemother 51: 651–656.
-
10.
Rason MA, Randriantsoa T, Andrianantenaina H, Ratsimbasoa A, Menard D, 2008. Performance and reliability of the SYBR Green I based assay for the routine monitoring of susceptibility of Plasmodium falciparum clinical isolates. Trans R Soc Trop Med Hyg 102:346–351.
-
11.
Desjardins RE, Canfield CJ, Haynes JD, Chulay JD, 1979. Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique. Antimicrob Agents Chemother 16: 710–718.
-
12.
Menard D, Djalle D, Manirakiza A, Yapou F, Siadoua V, Sana S, Matsika-Claquin MD, Nestor M, Talarmin A, 2005. Drug-resistant malaria in Bangui, Central African Republic: an in vitro assessment. Am J Trop Med Hyg 73: 239–243.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 836 | 438 | 145 |
| Full Text Views | 755 | 14 | 0 |
| PDF Downloads | 292 | 17 | 0 |