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Genotyping of Trypanosoma cruzi Sublineage in Human Samples from a North-East Argentina Area by Hybridization with DNA Probes and Specific Polymerase Chain Reaction (PCR)

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  • Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Cs Biológicas, Universidad Nacional del Litoral, Argentina; Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Chile; Hospital Central de Reconquista. Santa Fe, Argentina

We have evaluated blood samples of chronic and congenital Trypanosoma cruzi-infected patients from the city of Reconquista located in the northeast of Argentina where no information was previously obtained about the genotype of infecting parasites. Fourteen samples of congenital and 19 chronical patients were analyzed by hybridization with DNA probes of minicircle hypervariable regions (mHVR). In congenital patients, 50% had single infections with TcIId, 7% single infections with TcIIe, 29% mixed infections with TcIId/e, and 7% had mixed infections with TcIId/b and 7% TcIId/b, respectively. In Chronical patients, 52% had single infections with TcIId, 11% single infections with TcIIe, 26% had mixed infections with TcIId/e, and 11% had non-identified genotypes. With these samples, we evaluated the minicircle lineage-specific polymerase chain reaction assay (MLS-PCR), which involves a nested PCR to HVR minicircle sequences and we found a correlation with hybridization probes of 96.4% for TcIId and 54.8% for TcIIe.

Author Notes

*Address correspondence to Iván Marcipar, Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Cs Biológicas Universidad Nacional del Litoral, Santa Fe 3000, Argentina. E-mail: imarcipr@fbcb.unl.edu.ar

Financial support: This work received financial support from CAI+D 2009 PI 15-86, Universidad Nacional del Litoral and FONDECYT-Chile 1085154.

Disclosure: ISM and VG are researchers from the CONICET (Argentina).

Authors' addresses: Cristina Diez, Virginia Lorenz, Verónica Gonzalez, Andrea Racca, Iván Bontempi, and Iván Marcipar, Laboratorio de Tecnología Inmunológica, Facultad de Bioquímica y Cs Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina, E-mail: imarcipr@fbcb.unl.edu.ar. Silvia Ortiz and Aldo Solari, Program of Cellular and Molecular Biology, Biomedical Sciences Institute, Faculty of Medicine, University of Chile, Santiago, Chile, E-mail: asolari@med.uchile.cl. Silvia Manattini, Hospital Central de Reconquista, Santa Fe, Argentina, E-mail: equiroz@arnet.com.ar.

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