• 1

    Walsh DS, Portaels F, Meyers WM, 2008. Buruli ulcer (Mycobacterium ulcerans infection). Trans R Soc Trop Med Hyg 102 :969–978.

  • 2

    Portaels F, Silva MT, Meyers WM, 2009. Buruli ulcer. Clin Dermatol 27 :291–305.

  • 3

    George KM, Chatterjee D, Gunawardana G, Welty D, Hayman J, Lee R, Small PL, 1999. Mycolactone: a polyketide toxin from Mycobacterium ulcerans required for virulence. Science 283 :854–857.

    • Search Google Scholar
    • Export Citation
  • 4

    Walsh DS, Meyers WM, Portaels F, Lane JE, Mongkolsirichaikul D, Hussem K, Gosi P, Myint KS, 2005. High rates of apoptosis in human Mycobacterium ulcerans culture positive Buruli ulcer skin lesions. Am J Trop Med Hyg 73 :410–415.

    • Search Google Scholar
    • Export Citation
  • 5

    Rondini S, Mensah-Quainoo E, Troll H, Bodmer T, Pluschke G, 2003. Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA. J Clin Microbiol 41 :4231–4237.

    • Search Google Scholar
    • Export Citation
  • 6

    Stragier P, Ablordey A, Durnez L, Portaels F, 2007. VNTR analysis differentiates Mycobacterium ulcerans and IS2404 positive mycobacteria. Syst Appl Microbiol 30 :525–530.

    • Search Google Scholar
    • Export Citation
  • 7

    Stinear T, Davies JK, Jenkin GA, Portaels F, Ross BC, Oppedisano F, Purcell M, Hayman JA, Johnson PD, 2000. A simple PCR method for rapid genotype analysis of Mycobacterium ulcerans. J Clin Microbiol 38 :1482–1487.

    • Search Google Scholar
    • Export Citation
  • 8

    Stinear T, Ross BC, Davies JK, Marino L, Robins-Browne RM, Oppedisano F, Sievers A, Johnson PD, 1999. Identification and characterization of IS2404 and IS2606: two distinct repeated sequences for detection of Mycobacterium ulcerans by PCR. J Clin Microbiol 37 :1018–1023.

    • Search Google Scholar
    • Export Citation
  • 9

    Guimaraes-Peres A, Portaels F, de Rijk P, Fissette K, Pattyn SR, van Vooren J, Fonteyne P, 1999. Comparison of two PCRs for detection of Mycobacterium ulcerans. J Clin Microbiol 37 :206–208.

    • Search Google Scholar
    • Export Citation
  • 10

    Edgar RC, 2004. MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5 :113.

  • 11

    Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32 :1792–1797.

  • 12

    Meyers WM, Shelly WM, Connor DH, Meyers EK, 1974. Human Mycobacterium ulcerans infections developing at sites of trauma to skin. Am J Trop Med Hyg 23 :919–923.

    • Search Google Scholar
    • Export Citation
  • 13

    Johnson RC, Nackers F, Glynn JR, de Biurrun Bakedano E, Zinsou C, Aguiar J, Tonglet R, Portaels F, 2008. Association of HIV infection and Mycobacterium ulcerans disease in Benin. AIDS 22 :901–903.

    • Search Google Scholar
    • Export Citation
  • 14

    Nackers F, Johnson RC, Glynn JR, Zinsou C, Tonglet R, Portaels F, 2007. Environmental and health-related risk factors for Mycobacterium ulcerans disease (Buruli ulcer) in Benin. Am J Trop Med Hyg 77 :834–836.

    • Search Google Scholar
    • Export Citation
  • 15

    Pouillot R, Matias G, Wondje CM, Portaels F, Valin N, Ngos F, Njikap A, Marsollier L, Fontanet A, Eyangoh S, 2007. Risk factors for buruli ulcer: a case control study in Cameroon. PLoS Negl Trop Dis 1 :e101.

    • Search Google Scholar
    • Export Citation
  • 16

    Ablordey A, Kotlowski R, Swings J, Portaels F, 2005. PCR amplification with primers based on IS2404 and GC-rich repeated sequence reveals polymorphism in Mycobacterium ulcerans. J Clin Microbiol 43 :448–451.

    • Search Google Scholar
    • Export Citation
  • 17

    Demangel C, Stinear TP, Cole ST, 2009. Buruli ulcer: reductive evolution enhances pathogenicity of Mycobacterium ulcerans. Nat Rev Microbiol 7 :50–60.

    • Search Google Scholar
    • Export Citation
  • 18

    Stragier P, Ablordey A, Bayonne LM, Lugor YL, Sindani IS, Suykerbuyk P, Wabinga H, Meyers WM, Portaels F, 2006. Heterogeneity among Mycobacterium ulcerans isolates from Africa. Emerg Infect Dis 12 :844–847.

    • Search Google Scholar
    • Export Citation
  • 19

    Meyers WM, Tignokpa N, Priuli GB, Portaels F, 1996. Mycobacterium ulcerans infection (Buruli ulcer): first reported patients in Togo. Br J Dermatol 134 :1116–1121.

    • Search Google Scholar
    • Export Citation

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Clinical and Molecular Evidence for a Case of Buruli Ulcer (Mycobacterium ulcerans Infection) in Kenya

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  • 1 Kenya Medical Research Institute–Walter Reed Project, United States Army Medical Research Unit-Kenya, Kisumu, Kenya; Global Emerging Infections System, United States Army Medical Research Unit-Kenya, Nairobi, Kenya; Department of Environmental and Infectious Disease Sciences, Armed Forces Institute of Pathology, Washington, District of Columbia, United States; Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium

Mycobacterium ulcerans infection is an emerging disease that causes indolent, necrotizing skin lesions known as Buruli ulcer (BU) and occasional contiguous or metastatic bone lesions. Buruli ulcer is named after Buruli County in Uganda (east Africa), where an epidemic occurred in the 1960s. Today, BU is most common in central and west Africa. We describe clinical and molecular evidence for a case of BU in Kenya.

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