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-
1
Hay SI, Guerra CA, Tatem AJ, Noor AM, Snow RW, 2004. The global distribution and population at risk of malaria: past, present, and future. Lancet Infect Dis 4 :327–336.
-
2
Mendis K, Sina BJ, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 :97–106.
-
3
Sina BJ, 2002. Focus on Plasmodium vivax. Trends Parasitol 18 :287–289.
-
4
Baird JK, 2007. Neglect of Plasmodium vivax malaria. Trends Parasitol 23 :533–539.
-
5
Despommier DD, Gwadz RW, Hotez PJ, Knirsch CA, 2005. Parasitic Diseases. Fifth edition. New York: Apple Tree Productions LLT (ATP), 50–68.
-
6
Mayxay M, Pukrittayakamee S, Newton PN, White NJ, 2004. Mixed-species malaria infections in humans. Trends Parasitol 20 :233–240.
-
7
Snounou G, White NJ, 2004. The co-existence of Plasmodium: sidelights from falciparum and vivax malaria in Thailand. Trends Parasitol 20 :333–339.
-
8
Udomsangpetch R, Kaneko O, Chotivanich K, Sattabongkot J, 2008. Cultivation of Plasmodium vivax. Trends Parasitol 24 :85–88.
-
9
Suwanarusk R, Cooke BM, Dondorp AM, Silamut K, Sattabongkot J, White NJ, Udomsangpetch R, 2004. The deformability of red blood cells parasitized by Plasmodium falciparum and P. vivax. J Infect Dis 189 :190–194.
-
10
Pukrittayakamee S, Imwong M, Singhasivanon P, Stepniewska K, Day NJ, White NJ, 2008. Effects of different antimalarial drugs on gametocyte carriage in P. vivax malaria. Am J Trop Med Hyg 79 :378–384.
-
11
Snounou G, Beck HP, 1998. The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol Today 14 :462–467.
-
12
Ciceron L, Jaureguiberry G, Gay F, Danis M, 1999. Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment. J Clin Microbiol 37 :35–38.
-
13
Schoone GJ, Oskam L, Kroon NC, Schallig HD, Omar SA, 2000. Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification. J Clin Microbiol 38 :4072–4075.
-
14
Hermsen CC, Telgt DS, Linders EH, van de Locht LA, Eling WM, Mensink EJ, Sauerwein RW, 2001. Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR. Mol Biochem Parasitol 118 :247–251.
-
15
Schneider P, Schoone GJ, Schallig HD, Verhage D, Telgt D, Eling WM, Sauerwein RW, 2004. Quantification of Plasmodium falciparum gametocytes in differential stages of development by quantitative nucleic acid sequence-based amplification. Mol Biochem Parasitol 137 :35–41.
-
16
Mens PF, Schoone GJ, Kager PA, Schallig HD, 2006. Detection and identification of human Plasmodium species with real-time quantitative nucleic acid sequence-based amplification. Malar J 5 :80.
-
17
Schneider P, Wolters LR, Schoone GJ, Schallig HD, Sillekens P, Hermsen R, Sauerwein RW, 2005. Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J Clin Microbiol 43 :402–405.
-
18
Bousema JT, Schneider P, Gouagna LC, Drakeley CJ, Tostmann A, Houben R, Githure JI, Ord R, Sutherland CJ, Omar SA, Sauerwein RW, 2006. Moderate effect of artemisinin-based combination therapy on transmission of Plasmodium falciparum. J Infect Dis 193 :1151–1159.
-
19
Shekalaghe SA, Bousema JT, Kunei KK, Lushino P, Masokoto A, Wolters LR, Mwakalinga S, Mosha FW, Sauerwein RW, Drakeley CJ, 2007. Submicroscopic Plasmodium falciparum gametocyte carriage is common in an area of low and seasonal transmission in Tanzania. Trop Med Int Health 12 :547–553.
-
20
Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, Sauerwein RW, 2007. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg 76 :470–474.
-
21
Bharti AR, Chuquiyauri R, Brouwer KC, Stancil J, Lin J, Llanos-Cuentas A, Vinetz JM, 2006. Experimental infection of the neo-tropical malaria vector Anopheles darlingi by human patient-derived Plasmodium vivax in the Peruvian Amazon. Am J Trop Med Hyg 75 :610–616.
-
22
National Centre for Biotechnology Information BLAST search. Available at: http://blast.ncbi.nlm.nih.gov/Blast.cgi. Accessed February 2006.
-
23
Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J, 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28 :495–503.
-
24
Uttayamakul S, Likanonsakul S, Sunthornkachit R, Kuntiranont K, Louisirirotchanakul S, Chaovavanich A, Thiamchai V, Tanprasertsuk S, Sutthent R, 2005. Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection. J Virol Methods 128 :128–134.
-
25
Leslie T, Mayan MI, Hasan MA, Safi MH, Klinkenberg E, Whitty CJ, Rowland M, 2007. Sulfadoxine-pyrimethamine, chlorprogua-nil-dapsone, or chloroquine for the treatment of Plasmodium vivax malaria in Afghanistan and Pakistan: a randomized controlled trial. JAMA 297 :2201–2209.
-
26
Boyd MF, 1949. Vivax malaria. Sixty-five contributors. Malariology. A Comprehensive Survey of all Aspects of This Group of Diseases from a Global Standpoint. London: W.B. Saunders Company, 1027–1045.
-
27
Branch O, Casapia WM, Gamboa DV, Hernandez JN, Alava FF, Roncal N, Alvarez E, Perez EJ, Gotuzzo E, 2005. Clustered local transmission and asymptomatic Plasmodium falciparum and Plasmodium vivax malaria infections in a recently emerged, hypoendemic Peruvian Amazon community. Malar J 4 :27.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 17 | 17 | 3 |
| Full Text Views | 328 | 88 | 2 |
| PDF Downloads | 93 | 30 | 1 |
Quantitative Determination of Plasmodium vivax Gametocytes by Real-Time Quantitative Nucleic Acid Sequence-Based Amplification in Clinical Samples
Martijn Beurskens
Martijn Beurskens
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Pètra Mens
Pètra Mens
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Henk Schallig
Henk Schallig
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Din Syafruddin
Din Syafruddin
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Puji Budi Setia Asih
Puji Budi Setia Asih
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Rob Hermsen
Rob Hermsen
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Robert Sauerwein
Robert Sauerwein
Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; KIT Biomedical Research, Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, Amsterdam, The Netherlands; Centre for Infection and Immunity Amsterdam (CINEMA), Division of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Centre, Amsterdam, The Netherlands; Eijkman Institute for Molecular Biology, Jakarta, Indonesia
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Microscopic detection of Plasmodium vivax gametocytes, the sexual life stage of this malaria parasite, is insensitive because P. vivax parasitaemia is low. To detect and quantify gametocytes a more sensitive, quantitative real-time Pvs25-QT-NASBA based on Pvs25 mRNA was developed and tested in two clinical sample sets from three different continents. Pvs25-QT-NASBA is highly reproducible with low inter-assay variation and reaches sensitivity approximately 800 times higher than conventional microscopic gametocyte detection. Specificity was tested in 104 samples from P. vivax-, P. falciparum-, P. malariae-, and P. ovale-infected patients. All non-vivax samples were negative in the Pvs25-QT-NASBA; out of 74 PvS18-QT-NASBA positive samples 69% were positive in the Pvs25-QT-NASBA. In a second set of 136 P. vivax microscopically confirmed samples, gametocyte prevalence was 8%, whereas in contrast 66% were positive by Pvs25-QT-NASBA. The data suggest that the human P. vivax gametocyte reservoir is much larger when assessed by Pvs25-QT-NASBA than by microscopy.
ProCite
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BibTeX
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-
1
Hay SI, Guerra CA, Tatem AJ, Noor AM, Snow RW, 2004. The global distribution and population at risk of malaria: past, present, and future. Lancet Infect Dis 4 :327–336.
-
2
Mendis K, Sina BJ, Marchesini P, Carter R, 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64 :97–106.
-
3
Sina BJ, 2002. Focus on Plasmodium vivax. Trends Parasitol 18 :287–289.
-
4
Baird JK, 2007. Neglect of Plasmodium vivax malaria. Trends Parasitol 23 :533–539.
-
5
Despommier DD, Gwadz RW, Hotez PJ, Knirsch CA, 2005. Parasitic Diseases. Fifth edition. New York: Apple Tree Productions LLT (ATP), 50–68.
-
6
Mayxay M, Pukrittayakamee S, Newton PN, White NJ, 2004. Mixed-species malaria infections in humans. Trends Parasitol 20 :233–240.
-
7
Snounou G, White NJ, 2004. The co-existence of Plasmodium: sidelights from falciparum and vivax malaria in Thailand. Trends Parasitol 20 :333–339.
-
8
Udomsangpetch R, Kaneko O, Chotivanich K, Sattabongkot J, 2008. Cultivation of Plasmodium vivax. Trends Parasitol 24 :85–88.
-
9
Suwanarusk R, Cooke BM, Dondorp AM, Silamut K, Sattabongkot J, White NJ, Udomsangpetch R, 2004. The deformability of red blood cells parasitized by Plasmodium falciparum and P. vivax. J Infect Dis 189 :190–194.
-
10
Pukrittayakamee S, Imwong M, Singhasivanon P, Stepniewska K, Day NJ, White NJ, 2008. Effects of different antimalarial drugs on gametocyte carriage in P. vivax malaria. Am J Trop Med Hyg 79 :378–384.
-
11
Snounou G, Beck HP, 1998. The use of PCR genotyping in the assessment of recrudescence or reinfection after antimalarial drug treatment. Parasitol Today 14 :462–467.
-
12
Ciceron L, Jaureguiberry G, Gay F, Danis M, 1999. Development of a Plasmodium PCR for monitoring efficacy of antimalarial treatment. J Clin Microbiol 37 :35–38.
-
13
Schoone GJ, Oskam L, Kroon NC, Schallig HD, Omar SA, 2000. Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification. J Clin Microbiol 38 :4072–4075.
-
14
Hermsen CC, Telgt DS, Linders EH, van de Locht LA, Eling WM, Mensink EJ, Sauerwein RW, 2001. Detection of Plasmodium falciparum malaria parasites in vivo by real-time quantitative PCR. Mol Biochem Parasitol 118 :247–251.
-
15
Schneider P, Schoone GJ, Schallig HD, Verhage D, Telgt D, Eling WM, Sauerwein RW, 2004. Quantification of Plasmodium falciparum gametocytes in differential stages of development by quantitative nucleic acid sequence-based amplification. Mol Biochem Parasitol 137 :35–41.
-
16
Mens PF, Schoone GJ, Kager PA, Schallig HD, 2006. Detection and identification of human Plasmodium species with real-time quantitative nucleic acid sequence-based amplification. Malar J 5 :80.
-
17
Schneider P, Wolters LR, Schoone GJ, Schallig HD, Sillekens P, Hermsen R, Sauerwein RW, 2005. Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J Clin Microbiol 43 :402–405.
-
18
Bousema JT, Schneider P, Gouagna LC, Drakeley CJ, Tostmann A, Houben R, Githure JI, Ord R, Sutherland CJ, Omar SA, Sauerwein RW, 2006. Moderate effect of artemisinin-based combination therapy on transmission of Plasmodium falciparum. J Infect Dis 193 :1151–1159.
-
19
Shekalaghe SA, Bousema JT, Kunei KK, Lushino P, Masokoto A, Wolters LR, Mwakalinga S, Mosha FW, Sauerwein RW, Drakeley CJ, 2007. Submicroscopic Plasmodium falciparum gametocyte carriage is common in an area of low and seasonal transmission in Tanzania. Trop Med Int Health 12 :547–553.
-
20
Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, Sauerwein RW, 2007. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg 76 :470–474.
-
21
Bharti AR, Chuquiyauri R, Brouwer KC, Stancil J, Lin J, Llanos-Cuentas A, Vinetz JM, 2006. Experimental infection of the neo-tropical malaria vector Anopheles darlingi by human patient-derived Plasmodium vivax in the Peruvian Amazon. Am J Trop Med Hyg 75 :610–616.
-
22
National Centre for Biotechnology Information BLAST search. Available at: http://blast.ncbi.nlm.nih.gov/Blast.cgi. Accessed February 2006.
-
23
Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J, 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28 :495–503.
-
24
Uttayamakul S, Likanonsakul S, Sunthornkachit R, Kuntiranont K, Louisirirotchanakul S, Chaovavanich A, Thiamchai V, Tanprasertsuk S, Sutthent R, 2005. Usage of dried blood spots for molecular diagnosis and monitoring HIV-1 infection. J Virol Methods 128 :128–134.
-
25
Leslie T, Mayan MI, Hasan MA, Safi MH, Klinkenberg E, Whitty CJ, Rowland M, 2007. Sulfadoxine-pyrimethamine, chlorprogua-nil-dapsone, or chloroquine for the treatment of Plasmodium vivax malaria in Afghanistan and Pakistan: a randomized controlled trial. JAMA 297 :2201–2209.
-
26
Boyd MF, 1949. Vivax malaria. Sixty-five contributors. Malariology. A Comprehensive Survey of all Aspects of This Group of Diseases from a Global Standpoint. London: W.B. Saunders Company, 1027–1045.
-
27
Branch O, Casapia WM, Gamboa DV, Hernandez JN, Alava FF, Roncal N, Alvarez E, Perez EJ, Gotuzzo E, 2005. Clustered local transmission and asymptomatic Plasmodium falciparum and Plasmodium vivax malaria infections in a recently emerged, hypoendemic Peruvian Amazon community. Malar J 4 :27.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 17 | 17 | 3 |
| Full Text Views | 328 | 88 | 2 |
| PDF Downloads | 93 | 30 | 1 |