Utility of a Protein Fraction with Cathepsin L-Like Activity Purified from Cysticercus Fluid of Taenia solium in the Diagnosis of Human Cysticercosis

Mirko Zimic Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Mónica Pajuelo Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Daniel Rueda Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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César López Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Yanina Arana Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Yesenia Castillo Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Maritza Calderón Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Silvia Rodriguez Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Patricia Sheen Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Joseph M. Vinetz Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Armando Gonzales Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Héctor H. García Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Robert H. Gilman Laboratorios de Investigación y Desarrollo. Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Perú; Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Division of Infectious Diseases, Department of Medicine, University of California, San Diego, La Jolla California; School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Perú; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

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Neurocysticercosis, an endemic parasitic disease in most developing countries, is caused by Taenia solium and compromises the human central nervous system. Cathepsin L-like proteases are secreted by several parasites including T. solium and constitute important antigens for immunodiagnostics. A protein fraction with cathepsin L-like activity was purified from the cysticercus fluid by size exclusion and ion exchange chromatography. Cathepsin L-like activity was measured fluorometrically by detecting the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC. The purified protein fraction included antigens of 53 and 25 kD that were tested in a Western immunoblot and in an enzyme-linked immunosorbent assay (ELISA) for detection of human cysticercosis. The sensitivity of the Western immunoblot was 96% for patients infected with multiple cysts and 78% for patients with a single cyst. Specificity was 98%. The sensitivity of the ELISA was 98% in patients with multiple cysts and 84% in patients with a single cyst. Specificity was 92.7%.

Author Notes

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