- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- Imported dengue cases in Taiwan during 2003–2007.
- Serotype distributions of imported DENV strains during 2003–2007.
- Molecular characterization of imported DENV strains.
- Phylogenetic analysis of DENV-1 isolates.
- Phylogenetic analysis of DENV-2 isolates.
- Phylogenetic analysis of DENV-3 isolates.
- Phylogenetic analysis of DENV-4 isolates.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Gubler DJ, 1997. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. Gubler DJ, Kuno G, editors. Dengue and Dengue Hemorrhagic Fever. New York: CAB International, 1–22.
-
2
Gibbons RV, Vaughn DW, 2002. Dengue: an escalating problem. BMJ 324 :1563–1566.
-
3
Gubler DJ, 1998. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11 :480–496.
-
4
Teichmann D, Rogler G, Grobusch MP, Schuler-Maue W, Klein E, 1999. Imported dengue virus type 2 infection acquired during an outbreak in India. Eur J Clin Microbiol Infect Dis 18 :310–312.
-
5
Wichmann O, Lauschke A, Frank C, Shu PY, Niedrig M, Huang JH, Stark K, Jelinek T, 2005. Dengue antibody prevalence in German travelers. J Infect Dis 11 :762–765.
-
6
Xu G, Dong H, Shi N, Liu S, Zhou A, Cheng Z, Chen G, Liu J, Fang T, Zhang H, Gu C, Tan X, Ye J, Xie S, Cao G, 2007. An outbreak of dengue virus serotype 1 infection in Cixi, Ningbo, People’s Republic of China, 2004, associated with a traveler from Thailand and high density of Aedes albopictus. Am J Trop Med Hyg 76 :1182–1188.
-
7
Shu PY, Chien LJ, Chang SF, Su CL, Kuo YC, Liao TL, Ho MS, Lin TH, Huang JH, 2005. Fever screening at airports and imported dengue. Emerg Infect Dis 11 :460–462.
-
8
Ito M, Yamada K, Takasaki T, Pandey B, Nerome R, Tajima S, Morita K, Kurane I, 2007. Phylogenetic analysis of dengue viruses isolated from imported dengue patients: possible aid for determining the countries where infections occurred. J Travel Med 14 :233–244.
-
9
Huhtamo E, Uzcátegui NY, Siikamäki H, Saarinen A, Piiparinen H, Vaheri A, Vapalahti O, 2008. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg Infect Dis 14 :80–83.
-
10
Lindbäck H, Lindbäck J, Tegnell A, Janzon R, Vene S, Ekdahl K, 2003. Dengue fever in travelers to the tropics, 1998 and 1999. Emerg Infect Dis 9 :438–442.
-
11
Schwartz E, Moskovitz A, Potasman I, Peri G, Grossman Z, Alkan ML, 2000. Changing epidemiology of dengue fever in travelers to Thailand. Eur J Clin Microbiol Infect Dis 19 :784–786.
-
12
Huang JH, Liao TL, Chang SF, Su CL, Chien LJ, Kuo YC, Yang CF, Lin CC, Shu PY, 2007. Laboratory-based dengue surveillance in Taiwan, 2005: a molecular epidemiologic study. Am J Trop Med Hyg 77 :903–909.
-
13
Nukui Y, Tajima S, Kotaki A, Ito M, Takasaki T, Koike K, Kurane I, 2006. Novel dengue virus type 1 from travelers to Yap State, Micronesia. Emerg Infect Dis 12 :343–346.
-
14
Domingo C, Palacios G, Jabado O, Reyes N, Niedrig M, Gascón J, Cabrerizo M, Lipkin WI, Tenorio A, 2006. Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India. J Clin Microbiol 44 :1519–1529.
-
15
Shu PY, Chang SF, Yueh YY, Chow L, Chien LJ, Kuo YC, Sue CL, Liao TL, Lin TH, Huang JH, 2004. Current status of dengue diagnosis at the Center for Disease Control, Taiwan. Dengue Bull WHO/SEARO 28 :107–117.
-
16
Shu PY, Chang SF, Kuo YC, Yueh YY, Chien LJ, Sue CL, Lin TH, Huang JH, 2003. Development of group- and serotype-specific one-step SYBR green I real-time reverse transcription-PCR for dengue virus. J Clin Microbiol 41 :2408–2416.
-
17
Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH, 2003. Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10 :622–630.
-
18
Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24 :1596–1599.
-
19
A-Nuegoonpipat A, Berlioz-Arthaud A, Chow V, Endy T, Lowry K, Mai le Q, Ninh TU, Pyke A, Reid M, Reynes JM, Su Yun ST, Thu HM, Wong SS, Holmes EC, Aaskov J, 2004. Sustained transmission of dengue virus type 1 in the Pacific due to repeated introductions of different Asian strains. Virology 329 :505–512.
-
20
Twiddy SS, Farrar JJ, Vinh Chau N, Wills B, Gould EA, Gritsun T, Lloyd G, Holmes EC, 2002. Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology 298 :63–72.
-
21
Lanciotti RS, Lewis JG, Gubler DJ, Trent DW, 1994. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75 :65–75.
-
22
Klungthong C, Zhang C, Mammen MP Jr, Ubol S, Holmes EC, 2004. The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand. Virology 329 :168–179.
-
23
Zhang C, Mammen MP Jr, Chinnawirotpisan P, Klungthong C, Rodpradit P, Monkongdee P, Nimmannitya S, Kalayanarooj S, Holmes EC, 2005. Clade replacements in dengue virus serotypes 1 and 3 are associated with changing serotype prevalence. J Virol 79 :15123–15130.
-
24
Salda LT, Parquet MD, Matias RR, Natividad FF, Kobayashi N, Morita K, 2005. Molecular epidemiology of dengue 2 viruses in the Philippines: genotype shift and local evolution. Am J Trop Med Hyg 73 :796–802.
-
25
Bennett SN, Holmes EC, Chirivella M, Rodriguez DM, Beltran M, Vorndam V, Gubler DJ, McMillan WO, 2006. Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction. J Gen Virol 87 :885–893.
-
26
Foster JE, Bennett SN, Vaughan H, Vorndam V, McMillan WO, Carrington CV, 2003. Molecular evolution and phylogeny of dengue type 4 virus in the Caribbean. Virology 306 :126–134.
-
27
Carrington CV, Foster JE, Pybus OG, Bennett SN, Holmes EC, 2005. Invasion and maintenance of dengue virus type 2 and type 4 in the Americas. J Virol 79 :14680–14687.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2249 | 2133 | 65 |
| Full Text Views | 361 | 9 | 0 |
| PDF Downloads | 136 | 12 | 2 |
Molecular Characterization of Dengue Viruses Imported Into Taiwan during 2003–2007: Geographic Distribution and Genotype Shift
Pei-Yun Shu
Pei-Yun Shu
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Chien-Ling Su
Chien-Ling Su
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Tsai-Ling Liao
Tsai-Ling Liao
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Cheng-Fen Yang
Cheng-Fen Yang
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Shu-Fen Chang
Shu-Fen Chang
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Chien-Chou Lin
Chien-Chou Lin
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Mei-Chun Chang
Mei-Chun Chang
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Huai-Chin Hu
Huai-Chin Hu
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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Jyh-Hsiung Huang
Jyh-Hsiung Huang
Research and Diagnostic Center, Centers for Disease Control, Department of Health, Taipei, Taiwan, Republic of China
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We presented our surveillance results on imported dengue cases in Taiwan during 2003–2007. A total of 542 imported dengue patients were identified. The travelers were infected in 17 countries in Southeast Asia, the Indian subcontinent, East African islands, South Pacific islands, and Central America. Most of these imported cases were infected in Southeast Asian countries. Phylogenetic analyses were conducted to examine 288 imported dengue virus (DENV) strains introduced from 13 countries. The results provide an updated view on the geographic distribution and dynamic transmission of epidemic DENV stains circulated in Southeast Asian countries. Although the geographic distributions of genotypes of DENV-3 isolated from Southeast Asian countries remain unchanged, the introductions and local expansions of epidemic DENV-1, DENV-2, and DENV-4 strains into new areas in Asia were observed. These findings highlight the importance to strengthen laboratory-based dengue surveillance for better understanding of transmission dynamics and molecular evolution of DENVs.
Author Notes
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- Imported dengue cases in Taiwan during 2003–2007.
- Serotype distributions of imported DENV strains during 2003–2007.
- Molecular characterization of imported DENV strains.
- Phylogenetic analysis of DENV-1 isolates.
- Phylogenetic analysis of DENV-2 isolates.
- Phylogenetic analysis of DENV-3 isolates.
- Phylogenetic analysis of DENV-4 isolates.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Gubler DJ, 1997. Dengue and dengue hemorrhagic fever: its history and resurgence as a global public health problem. Gubler DJ, Kuno G, editors. Dengue and Dengue Hemorrhagic Fever. New York: CAB International, 1–22.
-
2
Gibbons RV, Vaughn DW, 2002. Dengue: an escalating problem. BMJ 324 :1563–1566.
-
3
Gubler DJ, 1998. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11 :480–496.
-
4
Teichmann D, Rogler G, Grobusch MP, Schuler-Maue W, Klein E, 1999. Imported dengue virus type 2 infection acquired during an outbreak in India. Eur J Clin Microbiol Infect Dis 18 :310–312.
-
5
Wichmann O, Lauschke A, Frank C, Shu PY, Niedrig M, Huang JH, Stark K, Jelinek T, 2005. Dengue antibody prevalence in German travelers. J Infect Dis 11 :762–765.
-
6
Xu G, Dong H, Shi N, Liu S, Zhou A, Cheng Z, Chen G, Liu J, Fang T, Zhang H, Gu C, Tan X, Ye J, Xie S, Cao G, 2007. An outbreak of dengue virus serotype 1 infection in Cixi, Ningbo, People’s Republic of China, 2004, associated with a traveler from Thailand and high density of Aedes albopictus. Am J Trop Med Hyg 76 :1182–1188.
-
7
Shu PY, Chien LJ, Chang SF, Su CL, Kuo YC, Liao TL, Ho MS, Lin TH, Huang JH, 2005. Fever screening at airports and imported dengue. Emerg Infect Dis 11 :460–462.
-
8
Ito M, Yamada K, Takasaki T, Pandey B, Nerome R, Tajima S, Morita K, Kurane I, 2007. Phylogenetic analysis of dengue viruses isolated from imported dengue patients: possible aid for determining the countries where infections occurred. J Travel Med 14 :233–244.
-
9
Huhtamo E, Uzcátegui NY, Siikamäki H, Saarinen A, Piiparinen H, Vaheri A, Vapalahti O, 2008. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg Infect Dis 14 :80–83.
-
10
Lindbäck H, Lindbäck J, Tegnell A, Janzon R, Vene S, Ekdahl K, 2003. Dengue fever in travelers to the tropics, 1998 and 1999. Emerg Infect Dis 9 :438–442.
-
11
Schwartz E, Moskovitz A, Potasman I, Peri G, Grossman Z, Alkan ML, 2000. Changing epidemiology of dengue fever in travelers to Thailand. Eur J Clin Microbiol Infect Dis 19 :784–786.
-
12
Huang JH, Liao TL, Chang SF, Su CL, Chien LJ, Kuo YC, Yang CF, Lin CC, Shu PY, 2007. Laboratory-based dengue surveillance in Taiwan, 2005: a molecular epidemiologic study. Am J Trop Med Hyg 77 :903–909.
-
13
Nukui Y, Tajima S, Kotaki A, Ito M, Takasaki T, Koike K, Kurane I, 2006. Novel dengue virus type 1 from travelers to Yap State, Micronesia. Emerg Infect Dis 12 :343–346.
-
14
Domingo C, Palacios G, Jabado O, Reyes N, Niedrig M, Gascón J, Cabrerizo M, Lipkin WI, Tenorio A, 2006. Use of a short fragment of the C-terminal E gene for detection and characterization of two new lineages of dengue virus 1 in India. J Clin Microbiol 44 :1519–1529.
-
15
Shu PY, Chang SF, Yueh YY, Chow L, Chien LJ, Kuo YC, Sue CL, Liao TL, Lin TH, Huang JH, 2004. Current status of dengue diagnosis at the Center for Disease Control, Taiwan. Dengue Bull WHO/SEARO 28 :107–117.
-
16
Shu PY, Chang SF, Kuo YC, Yueh YY, Chien LJ, Sue CL, Lin TH, Huang JH, 2003. Development of group- and serotype-specific one-step SYBR green I real-time reverse transcription-PCR for dengue virus. J Clin Microbiol 41 :2408–2416.
-
17
Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH, 2003. Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10 :622–630.
-
18
Tamura K, Dudley J, Nei M, Kumar S, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24 :1596–1599.
-
19
A-Nuegoonpipat A, Berlioz-Arthaud A, Chow V, Endy T, Lowry K, Mai le Q, Ninh TU, Pyke A, Reid M, Reynes JM, Su Yun ST, Thu HM, Wong SS, Holmes EC, Aaskov J, 2004. Sustained transmission of dengue virus type 1 in the Pacific due to repeated introductions of different Asian strains. Virology 329 :505–512.
-
20
Twiddy SS, Farrar JJ, Vinh Chau N, Wills B, Gould EA, Gritsun T, Lloyd G, Holmes EC, 2002. Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology 298 :63–72.
-
21
Lanciotti RS, Lewis JG, Gubler DJ, Trent DW, 1994. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 75 :65–75.
-
22
Klungthong C, Zhang C, Mammen MP Jr, Ubol S, Holmes EC, 2004. The molecular epidemiology of dengue virus serotype 4 in Bangkok, Thailand. Virology 329 :168–179.
-
23
Zhang C, Mammen MP Jr, Chinnawirotpisan P, Klungthong C, Rodpradit P, Monkongdee P, Nimmannitya S, Kalayanarooj S, Holmes EC, 2005. Clade replacements in dengue virus serotypes 1 and 3 are associated with changing serotype prevalence. J Virol 79 :15123–15130.
-
24
Salda LT, Parquet MD, Matias RR, Natividad FF, Kobayashi N, Morita K, 2005. Molecular epidemiology of dengue 2 viruses in the Philippines: genotype shift and local evolution. Am J Trop Med Hyg 73 :796–802.
-
25
Bennett SN, Holmes EC, Chirivella M, Rodriguez DM, Beltran M, Vorndam V, Gubler DJ, McMillan WO, 2006. Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction. J Gen Virol 87 :885–893.
-
26
Foster JE, Bennett SN, Vaughan H, Vorndam V, McMillan WO, Carrington CV, 2003. Molecular evolution and phylogeny of dengue type 4 virus in the Caribbean. Virology 306 :126–134.
-
27
Carrington CV, Foster JE, Pybus OG, Bennett SN, Holmes EC, 2005. Invasion and maintenance of dengue virus type 2 and type 4 in the Americas. J Virol 79 :14680–14687.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2249 | 2133 | 65 |
| Full Text Views | 361 | 9 | 0 |
| PDF Downloads | 136 | 12 | 2 |