• 1

    Gubler DJ, 1998. Dengue and dengue hemorrhagic fever. Clin Microbiol Rev 11 :480–496.

  • 2

    Oishi K, Saito M, Mapua CA, Natividad FF, 2007. Dengue illness: clinical features and pathogenesis. J Infect Chemother 13 :125–133.

  • 3

    Halstead SB, 1988. Pathogenesis of dengue: challenges of molecular biology. Science 239 :476–481.

  • 4

    Gubler DJ, 2002. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problems in the 21st century. Trends Microbiol 10 :100–103.

    • Search Google Scholar
    • Export Citation
  • 5

    La Russa VF, Innis BL, 1995. Mechanism of dengue virus-induced bone marrow suppression. Baillieres Clin Haematol 8 :249–270.

  • 6

    Cines DB, Blanchette VS, Chir B, 2002. Immune thrombocytopenic purpura. N Engl J Med 346 :995–1007.

  • 7

    Rand ML, Wright JF, 1998. Virus-associated idiopathic thrombocytopenic purpura. Transfus Sci 19 :253–259.

  • 8

    Oishi K, Inoue S, Cinco MT, Dimaano EM, Alera MT, Alfon JA, Abanes F, Cruz DJ, Matias RR, Matsuura H, Hasebe F, Tanimura S, Kumatori A, Morita K, Natividad FF, Nagatake T, 2003. Correlation between increased platelet-associated IgG and thrombocytopenia in secondary dengue virus infections. J Med Virol 71 :259–264.

    • Search Google Scholar
    • Export Citation
  • 9

    Saito M, Oishi K, Inoue S, Dimaano EM, Alera MTP, Robles MP, Estrella JR, Kumatori A, Moji K, Alonzo BMT, Buerano CC, Matias RR, Morita K, Natividad FF, Nagatake T, 2004. Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections. Clin Exp Immunol 138 :299–303.

    • Search Google Scholar
    • Export Citation
  • 10

    Wang S, He R, Patarapotikul J, Innis BL, Anderson R, 1995. Antibody-enhanced binding of dengue-2 virus to human platelet. Virology 213 :254–257.

    • Search Google Scholar
    • Export Citation
  • 11

    Berlioz-Arthaud A, Marfel M, Durand AM, Ogawa T, 2005. Evaluation of a new anti-dengue virus IgM particle agglutination kit in the context of the Pacific Islands. WHO Dengue Bulletin 29 :70–78.

    • Search Google Scholar
    • Export Citation
  • 12

    Morita K, Tanaka M, Igarashi A, 1991. Rapid identification of dengue virus serotypes by using polymerase chain reaction. J Clin Microbiol 29 :2107–2110.

    • Search Google Scholar
    • Export Citation
  • 13

    Clarke DH, Casals J, 1958. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg 7 :561–573.

    • Search Google Scholar
    • Export Citation
  • 14

    World Health Organization, 1997. Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention and Control (Second edition). Geneva, Switzerland: WHO.

  • 15

    Hoffmeister KM, Felbinger TW, Falet H, Denis CV, Bergmeier W, Nayadas TN, von Andrian UH, Wagner DD, Stossel TP, Hartwig JH, 2003. The clearance mechanism of chilled blood platelets. Cell 112 :87–97.

    • Search Google Scholar
    • Export Citation
  • 16

    Simon DI, Chen Z, Xu H, Li CQ, Dong J-F, McIntire LV, Ballantyne CM, Zhang L, Furman MI, Berndt MC, López JA, 2000. Platelet glycoprotein Ibα is a counterreceptor for leukocyte integrin Mac-1(CD11b/CD18). J Exp Med 192 :193–204.

    • Search Google Scholar
    • Export Citation
  • 17

    Mitrakul C, Poshyachinda M, Futralul P, Sangkawibha N, Ahandrik S, 1977. Hemostatic and platelet kinetic studies in dengue hemorrhagic fever. Am J Trop Med Hyg 26 :975–984.

    • Search Google Scholar
    • Export Citation
  • 18

    Bokisch VA, Top FH Jr, Russell PK, Dixon FJ, Muller-Eberhard HJ, 1973. The potential pathogenic role of complement in dengue hemorrhagic shock syndrome. N Engl J Med 289 :996–1000.

    • Search Google Scholar
    • Export Citation
  • 19

    Avirutnan P, Punyanadee N, Noisaran S, Komoltri C, Thiemmeca S, Auethavornanan K, Jairungsri A, Kanlaya R, Tangtaworn-chailul N, Puttikhunt C, Pattnakisakul S, Yenchitsomnus P, Mongkolsapaya J, Kasinrerk W, Sittisombut N, Husmann M, Blettner M, Vasanawathana S, Bhakdi S, Mlasit P, 2006. Vascular leakage in severe dengue virus infections: a potential role for the nonstructural viral protein NS1 and complement. J Infect Dis 193 :1078–1088.

    • Search Google Scholar
    • Export Citation
  • 20

    Dimaano E, Saito M, Honda S, Miranda EA, Alonzo MT, Valerio MD, Mapua CD, Inoue S, Kumatori A, Matias R, Natividad FF, Oishi K, 2007. Lack of efficacy of high dose intravenous immunoglobulin treatment of severe thrombocytopenia in patients with secondary dengue virus infection. Am J Trop Med Hyg 77 :1135–1138.

    • Search Google Scholar
    • Export Citation
  • 21

    Shcherbina A, Remold-O’Donnell E, 1999. Role of caspase in a subset of human platelet activation responses. Blood 93 :4222–4231.

  • 22

    Tonon G, Luo X, Greco NJ, Chen W, Shi Y, Jamieson GA, 2003. Weak platelet agonists and U46619 induces apoptosis-like events in the absence of phosphatidylserine exposure. Thromb Res 107 :345–350.

    • Search Google Scholar
    • Export Citation
  • 23

    Brown SB, Clarke MCH, Magowan L, Sanderson H, Savill J, 2000. Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. J Biol Chem 275 :5987–5996.

    • Search Google Scholar
    • Export Citation
 
 
 

 

 
 
 

 

 

 

 

 

 

Increased Phagocytosis of Platelets from Patients with Secondary Dengue Virus Infection by Human Macrophages

View More View Less
  • 1 Department of Clinical Medicine and Virology, Institute of Tropical Medicine Nagasaki University, Japan; Department of Virology, Graduate School of Medicine, Tohoku University, Japan; Department of Disaster Prevention System, Faculty of Risk and Crisis Management, Chiba Institute of Science, Japan; Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Japan; Department of Blood Borne Diseases, San Lazaro Hospital, Manila, Philippines; Research and Biotechnology Division, St. Luke’s Medical Center, Quezon City, Philippines

The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and the peripheral platelet counts as well as platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. The percent phagocytosis and the levels of PAIgG were significantly increased in these patients during the acute phase compared with the healthy volunteers. The increased percent phagocytosis and PAIgG found during the acute phase significantly decreased during the convalescent phase. An inverse correlation between platelet count and the percent phagocytosis (P = 0.011) and the levels of PAIgG (P = 0.041) was found among these patients during the acute phase. No correlation was found, however, between the percent phagocytosis and the levels of PAIgG. Our present data suggest that accelerated platelet phagocytosis occurs during the acute phase of secondary DV infections, and it is one of the mechanisms of thrombocytopenia in this disease.

Author Notes

Reprint requests: Kazunori Oishi, Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Osaka 565-0871, Japan, Tel: +81-6-6879-4253, Fax: +81-6-6879-4255, E-mail: oishik@biken.osaka-u.ac.jp.
Save