Comparison of Culture Techniques at Different Stages of Brucellosis

Benjamin J. Espinosa Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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Jesús Chacaltana Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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Maximilian Mulder Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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María Pía Franco Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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David L. Blazes Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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Robert H. Gilman Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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Henk L. Smits Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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Eric R. Hall Naval Medical Research Center Detachment, Lima, Peru; Hospital Nacional Daniel Alcides Carrion, Callao, Peru; A. B. Prisma, Lima, Peru; Department of Internal Medicine, Hennepin County Medical Center, Minneapolis, Minnesota; Department of Neurology, University of Minnesota, Minneapolis, Minnesota; Royal Tropical Institute, Amsterdam, The Netherlands

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The lysis centrifugation technique is preferred for culturing Brucella spp. at all stages of brucellosis because it yields 25% more positive results and on average provides results 10 days earlier than the Ruiz-Castaneda method. This lysis method is inexpensive and easier to use and may be used in laboratories with limited expertise or equipment if all safety precautions are taken.

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