Isolation of a Type 2 Dengue Virus by Use of Hamster Kidney Cell Cultures

Fred H. Diercks Department of Epidemiology and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania

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Attempts to recover dengue virus from 5 acute phase human serum specimens which had been stored at CO2 icebox temperatures for a period of 12 years were carried out in hamster kidney cell cultures. The one successful isolation attempt yielded a type 2 dengue fever virus. The importance of the procedures used for increasing the probability of recovery of viruses from virus-containing materials was discussed.

Author Notes

Major, MSC, now stationed at Walter Reed Army Institute of Research, Washington, D. C.