Rickettsia felis Outer Membrane Protein A: A Potential Tool for Diagnosis of Patients with Flea-Borne Spotted Fever

Jorge E. Zavala-Castro Facultad de Medicina, Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Universidad Autónoma de Yucatán, Mérida, Yucatán, México; Department of Pathology, University of Texas Medical Branch, Galveston, Texas

Search for other papers by Jorge E. Zavala-Castro in
Current site
Google Scholar
PubMed
Close
,
Karla R. Dzul-Rosado Facultad de Medicina, Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Universidad Autónoma de Yucatán, Mérida, Yucatán, México; Department of Pathology, University of Texas Medical Branch, Galveston, Texas

Search for other papers by Karla R. Dzul-Rosado in
Current site
Google Scholar
PubMed
Close
,
Juan José Arias León Facultad de Medicina, Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Universidad Autónoma de Yucatán, Mérida, Yucatán, México; Department of Pathology, University of Texas Medical Branch, Galveston, Texas

Search for other papers by Juan José Arias León in
Current site
Google Scholar
PubMed
Close
,
David H. Walker Facultad de Medicina, Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Universidad Autónoma de Yucatán, Mérida, Yucatán, México; Department of Pathology, University of Texas Medical Branch, Galveston, Texas

Search for other papers by David H. Walker in
Current site
Google Scholar
PubMed
Close
, and
Jorge E. Zavala-Velázquez Facultad de Medicina, Unidad Interinstitucional de Investigación Clínica y Epidemiológica, Universidad Autónoma de Yucatán, Mérida, Yucatán, México; Department of Pathology, University of Texas Medical Branch, Galveston, Texas

Search for other papers by Jorge E. Zavala-Velázquez in
Current site
Google Scholar
PubMed
Close
Restricted access

Rickettsia felis infection in humans has been detected worldwide, causing an illness that could be confused with other viral and bacterial infections such as dengue fever. Despite the high incidence of infection in humans, reservoirs, and vectors, the definitive diagnosis of R. felis infection currently requires polymerase chain reaction and sequencing because its antigens are closely related to other rickettsiae, making serologic diagnosis problematic. In this work, we report the immune reactivity of sera from four patients infected with R. felis with recombinant peptides representing regions of outer membrane protein A of R. felis.

Author Notes

  • 1

    CDC, 1998. Update. Leptospirosis and unexplained acute febrile illness among athletes participating in triathlons-Illinois and Wisconsin. MMWR Morb Mortal Wkly Rep 47 :673–676.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2

    Sexton DJ, Walker DH, 2006. Spotted fever group rickettsiosis. Guerrant RL, Walker DH, Weller PF, eds. Tropical Infectious Diseases, Principles, Pathogens, and Practice. Second edition. Philadelphia: Churchill Livingstone, 539–547.

    • PubMed
    • Export Citation
  • 3

    Zavala-Velázquez JE, Ruiz-Sosa JA, S ánchez-Elias RA, Becerra-Carmona G, Walker DH, 2000. Rickettsia felis rickettsiosis in Yucatán. Lancet 356 :1079–1080.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4

    Gillespie JJ, Beier MS, Rahman MS, Ammerman NC, Shallom JM, Purkayastha A, Sobral BS, Azad AF, 2007. Plasmids and rickettsial evolution: insight from Rickettsia felis. PLoS ONE 2 :e266.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5

    Bouyer DH, Stenos J, Crocquet-Valdes P, Moron CG, Popov VL, Zavala-Velázquez JE, Foil LD, Stothard DR, Azad AF, Walker DH, 2001. Rickettsia felis: molecular characterization of a new member of the spotted fever group. Int J Syst Evol Microbiol 51 :339–347.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6

    Zavala-Castro J, Small M, Keng C, Bouyer DH, Zavala-Velázquez JE, Walker DH, 2005. Transcription of the Rickettsia felis ompA gene in naturally infected fleas. Am J Trop Med Hyg 73 :662–666.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7

    Laemmli K, 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227 :680–682.

  • 8

    Towbin H, Staehelin T, Bordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 :4350–4354.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9

    Zavala-Velázquez J, Laviada-Molina H, Zavala-Castro J, Perez-Osorio C, Becerra-Carmona G, Ruiz-Sosa JA, Bouyer DH, Walker DH, 2006. Rickettsia felis, the agent of an emerging infectious disease: report of a new case in Mexico. Arch Med Res 37 :419–422.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10

    Adams JR, Schmidtmann ET, Azad AF, 1990. Infection of colonized cat fleas, Ctenocephalides felis (Bouché), with a rickettsia-like microorganism. Am J Trop Med Hyg 43 :400–409.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11

    Galvao MAM, Zavala-Velázquez JE, Zavala-Castro JE, Mafra CL, Calic SB, Walker DH. Rickettsia felis in the Americas, 2006. Ann NY Acad Sci 1078: 156–158.

  • 12

    Nogueras MM, Cardenosa N, Sanfeliu I, Munoz T, Font B, Segura F, 2006. Evidence of infection in humans with Rickettsia typhi and Rickettsia felis in Catalonia in the northeast of Spain. Ann NY Acad Sci 1078 :159–161.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13

    Oteo JA, Portillo A, Santibáñez S, Blanco JR, Pérez-Martínez L, Ibarra V, 2006. Cluster of cases of human Rickettsia felis infection from Southern Europe (Spain) diagnosed by PCR. J Clin Microbiol 44 :2669–2671.

    • PubMed
    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 401 334 25
Full Text Views 248 10 2
PDF Downloads 37 4 0
 

 

 

 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save