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1
Estripeaut D, Aramburú MG, Sáenz-Llorens X, Thompson HA, Dash G, Paddock SZ, Eremeeva ME, 2007. Rocky Mountain Spotted Fever, Panama. Emerg Infect Dis 13 :1763–1765.
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2
Hidalgo M, Orejuela L, Fuya P, Carrillo P, Hernández J, Parra E, Keng C, Small M, Olano JP, Bouyer D, Castaneda E, Walker D, Valbuena G, 2007. Rocky Mountain Spotted Fever, Colombia. Emerg Infect Dis 13 :1058–1060.
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3
Galvao M, Mafra C, Moron E, Walker D, 2003. Rickettsiosis of the genus Rickettsia in South America. Ann NY Acad Sci 990 :57–61.
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4
Dumler JS, Walker DH, 2005. Rocky Mountain Spotted Fever. Changing ecology and persisting virulence. N Engl J Med 353 :551–553.
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5
Fuentes LG, 1979. Primer caso de fiebre de las Montañas Rocosas en Costa Rica, América Central. Rev Latinoam Microbiol 21 :167–172.
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6
Hun L, Herrero L, Fuentes L, Vargas M, 1991. Tres nuevos casos de Fiebres Manchadas de las Montañas Rocosas en Costa Rica. Rev Costarric Cienc Med 12 :51–56.
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7
Philip RN, Burgdorfer W, Gerloff RK, Hughes LE, Bell EJ, 1978. Serologic typing of rickettsiae of the spotted fever group by microimmunofluorescense. J Immunol 121 :1961–1978.
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8
Fuentes L, Calderón A, Hun L, 1985. Isolation and identification of Rickettsia rickettsii from the rabbit tick Haemophysalis lepolispaustris in the Atlantic zone of Costa Rica. Am J Trop Med Hyg 34 :564–567.
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9
Parker RE, Pickens EG, Lackman DB, Bell EJ, Tharailkil FB, 1951. Isolation and characterization of Rocky Mountain Spotted fever from rabbit tick Haemaphysalis leporspalustris. Pub Health Rep 66 :455–463.
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10
Anacker RL, Philip RN, Williams JC, Lis RH, Mann RE, 1984. Biochemical and Immunochemical analysis of Rickettsia rickettsii strains of varius degrees of virulence. Infect Immun 44 :559–564.
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11
Karpathy ES, Dash GA, Eremeeva ME, 2007. Molecular typing of isolates of Rickettsia rickettsii using DNA sequencing of variable intergenic regions. J Clin Microbiol 45 :2545–2553.
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12
Fuentes LG, 1986. Ecological study of Rocky Mountain Spotted Fever in Costa Rica. Am J Trop Med Hyg 35 :192–196.
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13
Regnery RL, Spruill CL, Plikaytis BD, 1991. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol 173 :1576–1589.
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14
Roux V, Fournier PE, Raoult D, 1996. Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA. J Clin Microbiol 34 :2058–2065.
-
15
Labruna M, Whitworth T, Horta M, Bouyer D, McBride J, Pinter A, Popov V, Gennari M, Walker D, 2004. Rickettsia species infecting Amblyoma cooperi ticks from an area in the state of Sao Paulo, Brazil, where Brazilian Spotted Fever is endemic. J Clin Microbiol 42 :90–98.
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16
Kidd L, Hegarty B, Sexton D, Breitschwerdt E, 2006. Molecular characterization of Rickettsia rickettsii infecting dogs and people in North Carolina. Ann NY Acad Sci 1078 :400–409.
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17
Ellison DW, Clark TR, Studervant DE, Virtaneva K, Porcella SF, Hackstadt T, 2008. Genomic comparision of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa. Infect Immun 76 :542–550.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 189 | 131 | 14 |
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Molecular Characterization of Rickettsia rickettsii Isolated from Human Clinical Samples and from the Rabbit Tick Haemaphysalis leporispalustris Collected at Different Geographic Zones in Costa Rica
Laya Hun
Laya Hun
Investigation Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, San Pedro, San José, Costa Rica
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Ximena Cortés
Ximena Cortés
Investigation Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, San Pedro, San José, Costa Rica
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Lizeth Taylor
Lizeth Taylor
Investigation Center for Tropical Diseases (CIET), Faculty of Microbiology, University of Costa Rica, San Pedro, San José, Costa Rica
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Five strains of spotted fever group (SFG) rickettsiae previously isolated from human clinical cases and from the tick Haemaphysalis leporispalustris were used for molecular characterization in this study to establish their genetic relationship compared with the prototype Rickettsia rickettsii strain Sheila Smith. Samples were tested by polymerase chain reaction (PCR) targeting the rickettsial genes gtlA, ompA, and ompB. PCR products of the latter two genes were DNA sequenced and compared with available sequences in GenBank. The ompA partial sequences of the five Costa Rican isolates showed 100% identity to several R. rickettsii sequences available in GenBank, including the sequence of the virulent reference strain Sheila Smith, whereas the ompB partial sequences of the five Costa Rican isolates showed 99.8–100% identity to R. rickettsii sequences from GenBank. This study showed the first molecular detection of R. rickettsii isolates from Rocky Mountain Spotted Fever patients and from the rabbit tick H. leporispalustris in different geographical zones in Costa Rica.
Author Notes
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Estripeaut D, Aramburú MG, Sáenz-Llorens X, Thompson HA, Dash G, Paddock SZ, Eremeeva ME, 2007. Rocky Mountain Spotted Fever, Panama. Emerg Infect Dis 13 :1763–1765.
-
2
Hidalgo M, Orejuela L, Fuya P, Carrillo P, Hernández J, Parra E, Keng C, Small M, Olano JP, Bouyer D, Castaneda E, Walker D, Valbuena G, 2007. Rocky Mountain Spotted Fever, Colombia. Emerg Infect Dis 13 :1058–1060.
-
3
Galvao M, Mafra C, Moron E, Walker D, 2003. Rickettsiosis of the genus Rickettsia in South America. Ann NY Acad Sci 990 :57–61.
-
4
Dumler JS, Walker DH, 2005. Rocky Mountain Spotted Fever. Changing ecology and persisting virulence. N Engl J Med 353 :551–553.
-
5
Fuentes LG, 1979. Primer caso de fiebre de las Montañas Rocosas en Costa Rica, América Central. Rev Latinoam Microbiol 21 :167–172.
-
6
Hun L, Herrero L, Fuentes L, Vargas M, 1991. Tres nuevos casos de Fiebres Manchadas de las Montañas Rocosas en Costa Rica. Rev Costarric Cienc Med 12 :51–56.
-
7
Philip RN, Burgdorfer W, Gerloff RK, Hughes LE, Bell EJ, 1978. Serologic typing of rickettsiae of the spotted fever group by microimmunofluorescense. J Immunol 121 :1961–1978.
-
8
Fuentes L, Calderón A, Hun L, 1985. Isolation and identification of Rickettsia rickettsii from the rabbit tick Haemophysalis lepolispaustris in the Atlantic zone of Costa Rica. Am J Trop Med Hyg 34 :564–567.
-
9
Parker RE, Pickens EG, Lackman DB, Bell EJ, Tharailkil FB, 1951. Isolation and characterization of Rocky Mountain Spotted fever from rabbit tick Haemaphysalis leporspalustris. Pub Health Rep 66 :455–463.
-
10
Anacker RL, Philip RN, Williams JC, Lis RH, Mann RE, 1984. Biochemical and Immunochemical analysis of Rickettsia rickettsii strains of varius degrees of virulence. Infect Immun 44 :559–564.
-
11
Karpathy ES, Dash GA, Eremeeva ME, 2007. Molecular typing of isolates of Rickettsia rickettsii using DNA sequencing of variable intergenic regions. J Clin Microbiol 45 :2545–2553.
-
12
Fuentes LG, 1986. Ecological study of Rocky Mountain Spotted Fever in Costa Rica. Am J Trop Med Hyg 35 :192–196.
-
13
Regnery RL, Spruill CL, Plikaytis BD, 1991. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol 173 :1576–1589.
-
14
Roux V, Fournier PE, Raoult D, 1996. Differentiation of spotted fever group rickettsiae by sequencing and analysis of restriction fragment length polymorphism of PCR-amplified DNA of the gene encoding the protein rOmpA. J Clin Microbiol 34 :2058–2065.
-
15
Labruna M, Whitworth T, Horta M, Bouyer D, McBride J, Pinter A, Popov V, Gennari M, Walker D, 2004. Rickettsia species infecting Amblyoma cooperi ticks from an area in the state of Sao Paulo, Brazil, where Brazilian Spotted Fever is endemic. J Clin Microbiol 42 :90–98.
-
16
Kidd L, Hegarty B, Sexton D, Breitschwerdt E, 2006. Molecular characterization of Rickettsia rickettsii infecting dogs and people in North Carolina. Ann NY Acad Sci 1078 :400–409.
-
17
Ellison DW, Clark TR, Studervant DE, Virtaneva K, Porcella SF, Hackstadt T, 2008. Genomic comparision of virulent Rickettsia rickettsii Sheila Smith and avirulent Rickettsia rickettsii Iowa. Infect Immun 76 :542–550.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 189 | 131 | 14 |
| Full Text Views | 360 | 10 | 0 |
| PDF Downloads | 89 | 10 | 0 |