Rapid Identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti Life Stages Using Real-time Polymerase Chain Reaction Assays

Lydia A. Hill School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Lydia A. Hill in
Current site
Google Scholar
PubMed
Close
,
Joseph B. Davis School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Joseph B. Davis in
Current site
Google Scholar
PubMed
Close
,
George Hapgood School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by George Hapgood in
Current site
Google Scholar
PubMed
Close
,
Peter I. Whelan School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Peter I. Whelan in
Current site
Google Scholar
PubMed
Close
,
Greg A. Smith School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Greg A. Smith in
Current site
Google Scholar
PubMed
Close
,
Scott A. Ritchie School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Scott A. Ritchie in
Current site
Google Scholar
PubMed
Close
,
R. D. Cooper School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by R. D. Cooper in
Current site
Google Scholar
PubMed
Close
, and
Andrew F. van den Hurk School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland, Australia; Tropical Population Health Unit Network, Queensland Health, Cairns, Queensland, Australia; Medical Entomology, Centre for Disease Control, Department of Health and Community Services, Darwin, Northern Territory, Australia; Australian Army Malaria Institute, Gallipoli Barracks, Enoggera, Queensland, Australia; Virology, Forensic and Scientific Services, Queensland Health, Coopers Plains, Queensland, Australia

Search for other papers by Andrew F. van den Hurk in
Current site
Google Scholar
PubMed
Close
Restricted access

In 2005, a widespread infestation of Aedes albopictus was discovered in the Torres Strait, the region between northern Australia and New Guinea. To contain this species, an eradication program was implemented in 2006. However, the progress of this program is impeded by the difficulty of morphologically separating Ae. albopictus larvae from the endemic species Aedes scutellaris. In this study, three real-time TaqMan polymerase chain reaction assays that target the ribosomal internal transcribed spacer 1 region were developed to rapidly identify Aedes aegypti, Ae. albopictus, and Ae. scutellaris from northern Australia. Individual eggs, larvae, pupae, and adults, as well as the species composition of mixed pools were accurately identified. The assay method was validated using 703 field-collected specimens from the Torres Strait.

  • 1

    Benedict MQ, Levine RS, Hawley WA, Lounibos LP, 2007. Spread of the tiger: global risk of invasion by the mosquito Aedes albopictus. Vector Borne Zoonotic Dis 7 :76–85.

    • Search Google Scholar
    • Export Citation
  • 2

    Gratz NG, 2004. Critical review of the vector status of Aedes albopictus. Med Vet Entomol 18 :215–227.

  • 3

    Almeida AP, Baptista SS, Sousa CA, Novo MT, Ramos HC, Panella NA, Godsey M, Simões MJ, Anselmo ML, Komar N, Mitchell CJ, Ribeiro H, 2005. Bioecology and vectorial capacity of Aedes albopictus (Diptera: Culicidae) in Macao, China, in relation to dengue virus transmission. J Med Entomol 42 :419–428.

    • Search Google Scholar
    • Export Citation
  • 4

    Effler PV, Pang L, Kitsutani P, Vorndam V, Nakata M, Ayers T, Elm J, Tom T, Reiter P, Rigau-Perez JG, Hayes JM, Mills K, Napier M, Clark GG, Gubler DJ, 2005. Dengue fever, Hawaii, 2001–2002. Emerg Infect Dis 11 :742–749.

    • Search Google Scholar
    • Export Citation
  • 5

    Reiter P, Fontenille D, Paupy C, 2006. Aedes albopictus as an epidemic vector of chikungunya virus: another emerging problem? Lancet Infect Dis 6 :463–464.

    • Search Google Scholar
    • Export Citation
  • 6

    Vazeille M, Moutailler S, Coudrier D, Rousseaux C, Khun H, Huerre M, Thiria J, Dehecq JS, Fontenille D, Schuffenecker I, Despres P, Failloux AB, 2007. Two Chikungunya isolates from the outbreak of La Reunion (Indian Ocean) exhibit different patterns of infection in the mosquito, Aedes albopictus. PLoS ONE 2 :e1168.

    • Search Google Scholar
    • Export Citation
  • 7

    Rezza G, Nicoletti L, Angelini R, Romi R, Finarelli AC, Panning M, Cordioli P, Fortuna C, Boros S, Magurano F, Silvi G, Angelini P, Dottori M, Ciufolini MG, Majori GC, Cassone A, 2007. Infection with chikungunya virus in Italy: an outbreak in a temperate region. Lancet 370 :1840–1846.

    • Search Google Scholar
    • Export Citation
  • 8

    Whelan P, Hapgood G, 2001. A mosquito survey of Dili, East Timor, and implications for disease control. Arbovirus Res Aust 8 :405–416.

    • Search Google Scholar
    • Export Citation
  • 9

    Schoenig E, 1972. Distribution of 3 species of Aedes (Stegomyia) carriers of virus diseases on the main island of Papua and New Guinea. Philipp Sci 9 :61–82.

    • Search Google Scholar
    • Export Citation
  • 10

    Kay BH, Prakash G, Andre RG, 1995. Aedes albopictus and Aedes (Stegomyia) species in Fiji. J Am Mosq Control Assoc 11 :230–234.

  • 11

    Elliot SA, 1980. Aedes albopictus in the Solomon and Santa Cruz islands, South Pacific. Trans R Soc Trop Med Hyg 74 :747–748.

  • 12

    Cooper RD, Waterson DGE, Kupo M, Sweeney AW, 1994. Aedes albopictus (Skuse) (Diptera: Culicidae) in the Western Province of Papua New Guinea and the threat of its introduction to Australia. J Aust Entomol Assoc 33 :115–116.

    • Search Google Scholar
    • Export Citation
  • 13

    Russell RC, Williams CR, Sutherst RW, Ritchie SA, 2005. Aedes (Stegomyia) albopictus—a dengue threat for southern Australia. Comm Dis Intell 29 :296–298.

    • Search Google Scholar
    • Export Citation
  • 14

    Mitchell CJ, Gubler DJ, 1987. Vector competence of geographic strains of Aedes albopictus and Aedes polynesiensis and certain other Aedes (Stegomyia) mosquitoes for Ross River virus. J Am Mosq Control Assoc 3 :142–147.

    • Search Google Scholar
    • Export Citation
  • 15

    Ritchie SA, Moore P, Carruthers M, Williams C, Montgomery B, Foley P, Ahboo S, van den Hurk AF, Lindsay MD, Cooper B, Beebe N, Russell RC, 2006. Discovery of a widespread infestation of Aedes albopictus in the Torres Strait, Australia. J Am Mosq Control Assoc 22 :358–365.

    • Search Google Scholar
    • Export Citation
  • 16

    Rai KS, Pashley DP, Munstermann LE, 1982. Genetics of speciation in Aedine mosquitoes. Steiner WM, Tabachnick WJ, Rai KS, Narang S, eds. Recent Developments in the Genetics of Insect Disease Vectors. Champaign, Illinois: Stipes Publishers, 84–129.

  • 17

    Lee DJ, Hicks MM, Griffiths M, Debenham ML, Bryan JH, Russell RC, Geary M, Marks EN, 1987. The Culicidae of the Australasian Region, Vol. 4. Entomology Monograph No. 2. Canberra: Australian Government Publishing Service Press.

  • 18

    Huang YM, 1972. The subgenus Stegomyia of Aedes in Southeast Asia. I—The Scutellaris group of species. Contrib Am Entomol Inst 9 :1–109.

    • Search Google Scholar
    • Export Citation
  • 19

    Lamche GD, Whelan PI, 2003. Variability of larval identification characters of exotic Aedes albopictus (Skuse) intercepted in Darwin, Northern Territory. Comm Dis Intell 27 :105–109.

    • Search Google Scholar
    • Export Citation
  • 20

    Beebe NW, Whelan PI, van den Hurk AF, Ritchie SA, Corcoran S, Cooper RD, 2007. A polymerase chain reaction-based diagnostic to identify larvae and eggs of container mosquito species from the Australian region. J Med Entomol 44 :376–380.

    • Search Google Scholar
    • Export Citation
  • 21

    Sinclair D, 1992. The distribution of Aedes aegypti and dengue in Queensland, 1990–June 30, 1992. Arbovirus Res Aust 6 :323.

  • 22

    Beebe NW, Whelan PI, van den Hurk A, Ritchie SA, Cooper RD, 2005. Genetic diversity of the dengue vector Aedes aegypti in Australia and implications for future surveillance and mainland incursion monitoring. Comm Dis Intell 29 :299–304.

    • Search Google Scholar
    • Export Citation
  • 23

    Beebe NW, Cooper RD, Foley DH, Ellis JT, 2000. Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms. Heredity 84 :244–253.

    • Search Google Scholar
    • Export Citation
  • 24

    Heid CA, Stevens J, Livak KJ, Williams PM, 1996. Real time quantitative PCR. Genome Res 6 :986–994.

  • 25

    Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K, 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4 :357–362.

    • Search Google Scholar
    • Export Citation
  • 26

    Smith G, Smith I, Harrower B, Warrilow D, Bletchly C, 2006. A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents. J Virol Methods 135 :229–234.

    • Search Google Scholar
    • Export Citation
  • 27

    Rutledge RG, Côté C, 2003. Mathematics of quantitative kinetic PCR and the application of standard curves. Nucleic Acids Res 31 :e93.

    • Search Google Scholar
    • Export Citation
  • 28

    Bass C, Williamson MS, Wilding CS, Donnelly MJ, Field LM, 2007. Identification of the main malaria vectors in the Anopheles gambiae species complex using a TaqMan real-time PCR assay. Malar J 6 :155.

    • Search Google Scholar
    • Export Citation
  • 29

    Marks EN, 1980. Mosquitoes (Diptera: Culicidae) of Cape York Peninsula, Australia. Stevens NC, Bailey A, eds. Contemporary Cape York Peninsula. Brisbane: The Royal Society of Queensland, 59–76.

  • 30

    Sanogo YO, Kim CH, Lampman R, Novak RJ, 2007. A real-time TaqMan polymerase chain reaction for the identification of Culex vectors of West Nile and Saint Louis encephalitis viruses in North America. Am J Trop Med Hyg 77 :58–66.

    • Search Google Scholar
    • Export Citation
Past two years Past Year Past 30 Days
Abstract Views 42 42 6
Full Text Views 424 139 0
PDF Downloads 247 71 0
 
Membership Banner
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save