Serum Levels of Cytokines in Two Ethnic Groups with Dengue Virus Infection

Berta N. Restrepo Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Ruth E. Ramirez Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Margarita Arboleda Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Gonzalo Alvarez Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Marta Ospina Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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Francisco J. Diaz Instituto Colombiano de Medicina Tropical, Universidad CES, Sabaneta, Antioquia, Colombia; Facultad de Odontología, Universidad CES, Medellín, Antioquia, Colombia; Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Laboratorio Departamental de Salud Publica, Direccion Seccional de Salud de Antioquia, Medellín, Colombia; Grupo Inmunovirologia, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia

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This study compared the serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interferon gamma (IFN-γ) in 78 Colombian patients, from two ethnic groups, with dengue virus infection. TNF-α levels were significantly higher in Afro-Colombians than in Mestizos and IL-6 levels were significantly higher in Mestizos than in Afro-Colombians, during the acute phase. IFN-γ levels were similar in both ethnic groups. Significantly higher TNF-α levels were found in Afro-Colombians than in Mestizos in both dengue fever (DF) and dengue hemorrhagic fever (DHF). The IL-6 levels were higher in Mestizos than in Afro-Colombians among patients with DF, but levels of this cytokine were higher in Afro-Colombians than in Mestizos among patients with DHF. Levels of IFN-γ were higher in patients with DHF than DF. Higher levels of these cytokines were observed in secondary infection. These results suggest that ethnicity may contribute to differences in immune responses to dengue infections.

Author Notes

  • 1

    Guha-Sapir D, Schimmer B, 2005. Dengue fever: new paradigms for a changing epidemiology. Emerg Themes Epidemiol 2 :1.

  • 2

    Kautner I, Robinson MJ, Kuhnle U, 1997. Dengue virus infection: epidemiology, pathogenesis, clinical presentation, diagnosis and prevention. J Pediatr 131 :516–524.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3

    Cardier JE, Mariño E, Romano E, Taylor P, Liprandi F, Bosch N, Rothman AL, 2005. Proinflammatory factors present in sera from patients with acute dengue infection induce activation and apoptosis of human microvascular endothelial cells: possible roll of TNF-α in endothelial cell damage in dengue. Cytokin 30 :359–365.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4

    Rico-Hesse R, Harrison LM, Salas RA, Tovar D, Nisalak A, Ramos C, Boshell J, de Mesa MT, Nogueira RM, da Rosa AT, 1997. Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230 :244–251.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5

    Rosen L, 1997. The emperor’s new clothes revisited or reflections on the pathogenesis of dengue hemorrhagic fever. Am J Trop Med Hyg 26 :337–343.

  • 6

    Halstead SB, 1988. Pathogenesis of dengue. Challenges to molecular biology. Science 238 :476–481.

  • 7

    Kurane I, Ennis FA, 1997. Immunopathogenesis of dengue virus infections. Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. Wallingford, UK: CABI Publishing, CAB International, 273–290.

    • PubMed
    • Export Citation
  • 8

    Rothman AL, Ennis FA, 1999. Immunopathogenesis of dengue hemorrhagic fever. Virology 257 :1–6.

  • 9

    Kurane I, Meager A, Ennis FA, 1986. Induction of interferon alpha and gamma from human lymphocytes bye dengue virus-infected cells. J Med Virol 67 (pt8):1653–1661.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10

    Hober D, Poli L, Roblin B, Gestas P, Chunge E, Granic G, Imbert P, Pecarere JL, Vergez-Pascal R, Wattre P, 1993. Serum levels of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-1beta (IL-1beta) in dengue-infected patients. Am J Trop Med Hyg 48 :324–331.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11

    Hober D, Nguyen TL, Shen L, Ha DQ, Huong VT, Benyoucef S, Nguyen TH, Bui TM, Loan HK, Le BL, Bouzidi A, De Groote D, Drouet MT, Deubel V, Wattre P, 1998. Tumor necrosis factor alpha levels in plasma and whole-blood culture in dengue-infected patients: relationship between virus detection and pre-existing specific antibodies. J Med Virol 54 :210–218.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12

    Kuno G, Bailey RE, 1994. Cytokine responses to dengue infection among Puerto Rican patients. Mem Inst Oswaldo Cruz 89 :179–182.

  • 13

    Chaturvedi UC, Agarwal R, Elbishbishi EA, Mustafa AS, 2000. Cytokine cascade in dengue hemorrhagic fever: implications for pathogenesis. FEMS Immunol Med Microbiol 28 :183–188.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14

    Chaturvedy U, Nagar R, Shrivastava R, 2006. Dengue and dengue haemorrhagic fever: implications of host genetics. FEMS Immunol Med Microbiol 47 :155–166.

  • 15

    Rothman AL, 2003. Immunology and immunopathogenesis of dengue disease. Adv Virus Res 60 :397–419.

  • 16

    Chaturvedi UC, Elbishbishi EA, Agarwal R, Raghupathy R, Nagar R, Tandon R, Pacsa AS, Younis OI, Azizieh F, 1999. Sequential production of cytokines by dengue virus-infected human peripheral blood leukocyte cultures. J Med Virol 59 :335–340.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 17

    Halstead SB, Streit TG, Lafontant JG, Putvatana R, Russell K, Sun W, Kanesa.Tasan N, Hayes CG, Watts DM, 2001. Haiti: absence of dengue hemorrhagic fever despite hyperendemic dengue virus transmission. Am J Trop Med Hyg 65 :180–183.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18

    Bravo J, Guzman MG, Kouri G, 1987. Why dengue hemorrhagic in Cuba? Individual risk factors for dengue hemorrhagic fever/ dengue shock syndrome. Trans R Soc Trop Med Hyg 8 :816–820.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 19

    Guzman MG, Kouri GP, Vazquez S, Rosario D, Bravo JR, Valdez L, 1999a. DHF epidemics in Cuba, 1981 and 1987: some interesting observations. Dengue Bull WHO 23 :39–43.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 20

    Guzman MG, Kourí GP, Valdéz L, Bravo J, Alvarez M, Vázquez S, Delgado I, Halstead SB, 2000. Epidemiological studies of dengue in Santiago de Cuba, 1997. Am J Epid 152 :793–799.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 21

    de la Sierra B, Kouri G, Guzmán MG, 2007. Race: a risk factor for dengue hemorrhagic fever. Arch Virol 152 :533–542.

  • 22

    Organización Panamericana de la Salud, 1995. Dengue y Dengue Hemorrágico en las Américas: Guías para su Prevención y Control. Publicación Científica No. 578.

    • PubMed
    • Export Citation
  • 23

    Blanton RE, Silva LK, Morato VG, Parrado AR, Dias JP, Melo PR, Reis EA, Goddard KA, Nunes MR, Rodrigues SG, Vasconcelos PF, Castro JM, Reis MG, Barreto ML, Teixeira MG, 2000. Genetic ancestry and income are associated with dengue hemorrhagic fever in a highly admixed population. Eur J Hum Gene 16 :762–765.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 24

    Juffrie M, Meer GM, Hack CE, Haasnoot K, Sutaryo XX, Veerman AJP, 2001. Inflammatory mediators in dengue virus infection in children interleukin-6 and its relation to C-reactive protein and secret phospholipase A2. Am J Trop Med Hyg 65 :70–75.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 25

    Orrego JC, 2004. Citoquinas. Rugeles MT, Patiño PJ. eds. Inmunología. Una Ciencia Activa. Colombia: Biogenesis, 325–334.

    • PubMed
    • Export Citation
  • 26

    Malavige GN, Fernando S, Fernando DJ, Seneviratne SL, 2004. Dengue viral infections. Postgrad Med J 80 :588–601.

  • 27

    Sonnerborg A, Ayehunie S, Julander I, 1995. Elevated levels of circulating tumor necrosis alpha in human immunodeficiency virus type 1-infected Africans living in Sweden. Clin Diagn Lab Immunol 2 :118–119.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 28

    Kimball T, Eslwick RK, Shiffman M, 2001. Ethnicity and cytokine production gauge responses of patients with hepatitis C to interferon-alpha therapy. J Med Virol 65 :510–516.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 29

    Velez DR, Menon R, Thorsen P, Jiang L, Simhan H, Morgan N, Fortunato SJ, Williams SM, 2007. Ethnic differences in interleukin 6 (IL-6) and IL6 receptor genes in spontaneous preterm birth and effects on amniotic fluid protein levels. Ann Hum Genet 71 :586–600.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 30

    de la Sierra B, Garcia G, Perez AB, Morier L, Alvarez M, Kouri G, Guzman MG, 2006. Ethnicity and difference in dengue virus-specific memory T cell responses in Cuban individuals. Viral Immunol 19 :662–668.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 31

    Sugimoto K, Stadanlick J, Ikeda F, Brensinger C, Furth EE, Alter HJ, Chang KM, 2003. Influence of ethnicity in the outcome of hepatitis C virus infection and cellular immune response. Hepatol 37 :590–599.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 32

    Kittigul L, Temprom W, Sujirarat D, Kittigul C, 2000. Determination of tumour necrosis factor-alpha levels in dengue virus infected by sensitive biotin-streptavidin enzyme-linked immunosorbent assay. J Virol Methods 90 :51–57.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 33

    Gagnon SJ, Mori M, Kurane I, Green S, Vaughn DW, Kalayanarooj S, Suntayakorn S, Ennis FA, Rothman AL, 2002. Cytokine gene expression and protein production in peripheral blood mononuclear cells of children with acute dengue virus infections. J Med Virol 67 :41–46.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 34

    Braga EL, Moura P, Pinto LM, Ignácio SR, Oliveira MJ, Cordeiro MT, Kubelka CF, 2001. Detection of circulant tumor necrosis factor-alpha, soluble tumor necrosis factor p75 and interferon-gamma in Brazilian patients with dengue fever and dengue hemorrhagic fever. Mem Inst Oswaldo Cruz 96 :229–232.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 35

    Bethell D, Flobbe K, Than Phuong CX, Day N, Pham TP, Buurman WA, Cardosa MJ, White NJ, Kwiatkowski D, 1998. Pathophysiologic and prognosis role of cytokines in dengue hemorrhagic fever. J Infect Dis 177 :778–780.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 36

    Restrepo BN, Isaza DM, Salazar CL, Ramirez R, Ospina M, Alvarez LG, 2008. Serum levels of interleukin-6, tumor necrosis-alpha and interferon-gamma in infants with and without dengue. Rev Soc Bras Med Trop 41 :6–10.

    • PubMed
    • Search Google Scholar
    • Export Citation
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