Evaluation of Two New Immunochromatographic Assays for Diagnosis of Malaria

Arsène Ratsimbasoa Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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Laza Fanazava Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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Rogelin Radrianjafy Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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Julien Ramilijaona Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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Hughes Rafanomezantsoa Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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Didier Ménard Malaria Research Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; Primary Health Centre of Ampasimpotsy, Ampasimpotsy, Madagascar

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We assessed the performance of two new commercially available rapid diagnostic tests (RDTs) for malaria (SD Bioline Malaria Ag Pf® test and Ag Pf/Pan® test) in 200 patients with uncomplicated malaria between August and October 2007 in Madagascar. Results of the two RDTs were compared with those obtained by microscopy and real-time polymerase chain reaction. The sensitivity and specificity for detection of Plasmodium falciparum were 93% and 98.9%, respectively, for the SD Bioline Malaria Ag Pf® test and 92.9% and 98.9% for the SD Bioline Malaria Ag Pf/Pan® test. The sensitivity of the SD Bioline Malaria Ag Pf/Pan® test was much lower for detection of other species (63.6%). The sensitivity of the two new assays decreased to 77.3% at parasitemia levels < 100 parasites/μL for detection of P. falciparum.

Author Notes

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