Authors:
, , , , , , , , , , , , , , , , , and
- INTRODUCTION
- MATERIALS AND METHODS
- Study area
- Baseline seroprevalence study
- Seroconversion study
- Surveillance of acute febrile illnesses
- Antibody detection by ELISA
- Antibody detection by plaque reduction neutralization tests
- RNA extraction, reverse transcriptase-polymerase chain reaction, and complementary DNA sequencing
- Dengue virus isolation
- Definitions
- Data analysis
- Ethical considerations
- RESULTS
- DISCUSSION
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1
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Risk Factors for Dengue Virus Infection in Rural Amazonia: Population-based Cross-sectional Surveys
Mônica da Silva-Nunes
Mônica da Silva-Nunes
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Vanda A. F. de Souza
Vanda A. F. de Souza
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Cláudio S. Pannuti
Cláudio S. Pannuti
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Márcia A. Sperança
Márcia A. Sperança
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Ana Carolina B. Terzian
Ana Carolina B. Terzian
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Maurício L. Nogueira
Maurício L. Nogueira
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Anna M. Y. Yamamura
Anna M. Y. Yamamura
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Marcos S. Freire
Marcos S. Freire
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Natal S. da Silva
Natal S. da Silva
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Rosely S. Malafronte
Rosely S. Malafronte
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Pascoal T. Muniz
Pascoal T. Muniz
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Helena B. Vasconcelos
Helena B. Vasconcelos
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Eliana V. P. da Silva
Eliana V. P. da Silva
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Pedro F. C. Vasconcelos
Pedro F. C. Vasconcelos
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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Marcelo U. Ferreira
Marcelo U. Ferreira
Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Laboratory of Virology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Laboratory of Molecular Biology, Marília Medical School, Marília, Brazil; Laboratory of Virology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, Brazil; Institute of Technology in Immunobiologicals, Oswaldo Cruz Foundation, Riuo de Janeiro, Brazil; Laboratory of Protozoology, Institute of Tropical Medicine of São Paulo, São Paulo, Brazil; Department of Health Sciences, Federal University of Acre, Rio Branco, Brazil; Laboratory of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Belém, Brazil
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A comparison of dengue virus (DENV) antibody levels in paired serum samples collected from predominantly DENV-naive residents in an agricultural settlement in Brazilian Amazonia (baseline seroprevalence, 18.3%) showed a seroconversion rate of 3.67 episodes/100 person-years at risk during 12 months of follow-up. Multivariate analysis identified male sex, poverty, and migration from extra-Amazonian states as significant predictors of baseline DENV seropositivity, whereas male sex, a history of clinical diagnosis of dengue fever, and travel to an urban area predicted subsequent seroconversion. The laboratory surveillance of acute febrile illnesses implemented at the study site and in a nearby town between 2004 and 2006 confirmed 11 DENV infections among 102 episodes studied with DENV IgM detection, reverse transcriptase-polymerase chain reaction, and virus isolation; DENV-3 was isolated. Because DENV exposure is associated with migration or travel, personal protection measures when visiting high-risk urban areas may reduce the incidence of DENV infection in this rural population.
Author Notes
- INTRODUCTION
- MATERIALS AND METHODS
- Study area
- Baseline seroprevalence study
- Seroconversion study
- Surveillance of acute febrile illnesses
- Antibody detection by ELISA
- Antibody detection by plaque reduction neutralization tests
- RNA extraction, reverse transcriptase-polymerase chain reaction, and complementary DNA sequencing
- Dengue virus isolation
- Definitions
- Data analysis
- Ethical considerations
- RESULTS
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Tauil PL, 2006. Perspectivas de controle de doenças transmitidas por vetores no Brasil. Rev Soc Bras Med Trop 39 :275–277.
-
2
Siqueira JB, Martelli CMT, Coelho GE, Simplício ACR, Hatch DL, 2005. Dengue and dengue hemorrhagic fever, Brazil, 1981–2002. Emerg Infect Dis 11 :48–53.
-
3
Torres JR, Castro J, 2007. The health and economic impact of dengue in Latin America. Cad Saude Publica 23 (Suppl. 1):S23–S31.
-
4
Chareonsook O, Foy HM, Teeraratkul A, Silarug N, 1999. Changing epidemiology of dengue hemorrhagic fever in Thailand. Epidemiol Infect 122 :161–166.
-
5
Strickman D, Sithiprasasna R, Kittayapong P, Innis BL, 2000. Distribution of dengue and Japanese encephalitis virus among children in rural and suburban Thai villages. Am J Trop Med Hyg 63 :27–35.
-
6
Lian CW, Seng CM, Chai WY, 2006. Spatial, environmental and entomological risk factor analysis on a rural dengue outbreak in Lundu District in Sarawak, Malaysia. Trop Biomed 23 :85–96.
-
7
Kumar A, Sharma SK, Padbidri VS, Thakare JP, Jain DC, Datta KK, 2001. An outbreak of dengue fever in rural areas of northern India. J Commun Dis 33 :274–281.
-
8
Tewari SC, Thenmozhi V, Katholi CR, Manavalan R, Munirathinam A, Gajanana A, 2004. Dengue vector prevalence and virus infection in a rural area in south India. Trop Med Int Health 9 :499–507.
-
9
Hayes CG, Phillips IA, Callahan JD, Griebenow WF, Hyams KC, Wu SJ, Watts DM, 1996. The epidemiology of dengue virus infection among urban, jungle, and rural populations in the Amazon Region of Peru. Am J Trop Med Hyg 55 :459–463.
-
10
Reiskind MH, Baisley KJ, Calampa C, Sharp TW, Watts DM, Wilson ML, 2001. Epidemiological and ecological characteristics of past dengue virus infection in Santa Clara, Peru. Trop Med Int Health 6 :212–218.
-
11
Silva-Nunes M, Malafronte Rdos S, Luz Bde A, Souza EA, Martins LC, Rodrigues SG, Chiang JO, Vasconcelos PF, Muniz PT, Ferreira MU, 2006. The Acre project: the epidemiology of malaria and arthropod-borne virus infections in a rural Amazonian population. Cad Saude Publica 22 :1325–1334.
-
12
Filmer D, Pritchett LH, 2001. Estimating wealth effects without expenditure data-or tear: an application to educational enrolments in states of India. Demography 38 :115–132.
-
13
Trape JF, 1985. Rapid evaluation of malaria parasite density and standardization of thick smear examination for epidemiological investigations. Trans R Soc Trop Med Hyg 79 :181–184.
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