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Natural Infection of Lutzomyia tortura with Leishmania (Viannia) naiffi in an Amazonian Area of Ecuador

Hirotomo KatoDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Eduardo A. GomezDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Yu-ichi YamamotoDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Manuel CalvopiñaDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Angel G. GuevaraDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Jorge D. MarcoDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Paola A. BarrosoDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Hiroyuki IwataDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Yoshihisa HashiguchiDepartment of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan; Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria, Ministerio de Salud Publica, Quito, Ecuador; Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan; Biologia Molecular, ESPE-Biotechnologia, Quito, Ecuador; Unidad de Medicina Tropical y Parasitologia, Centro de Biomedicina, Universidad Central, Quito, Ecuador; Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan

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Natural infection of sand flies with Leishmania parasites was surveyed in an Amazonian area in Ecuador where leishmaniasis is endemic. Seventy-one female sand flies were dissected and one was positive for Leishmania protozoa. The species of this sand fly was identified as Lutzomyia (Lu.) tortura on the basis of morphologic characteristics. Analysis of the cytochrome b gene sequence identified the parasite as L. (Viannia) naiffi. We report the distribution of L. (V.) naiffi in Ecuador and detection of a naturally infected sand fly in the Ecuadorian Amazon and natural infection of Lu. tortura with Leishmania parasites in the New World.

Author Notes

Reprint requests: Hirotomo Kato, Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan, E-mail: katoh@yamaguchi-u.ac.jp.
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