Drinking Water: A Possible Source of Blastocystis spp. Subtype 1 Infection in Schoolchildren of a Rural Community in Central Thailand

Saovanee Leelayoova Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Suradej Siripattanapipong Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Umaporn Thathaisong Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Tawee Naaglor Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Paanjit Taamasri Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Phunlerd Piyaraj Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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Mathirut Mungthin Department of Parasitology, Phramongkutklao College of Medicine, Ratchathewi, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand

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In January 2005, a survey of intestinal parasitic infections was performed in a primary school, central Thailand. Of 675 stool samples, Blastocystis was identified with a prevalence of 18.9%. Genetic characterization of Blastocystis showed subtype 1 (77.9%) and subtype 2 (22.1%). Study of the water supply in this school was performed to find the possible sources of Blastocystis. Blastocystis from one water sample was identified as subtype 1, which had a nucleotide sequence of small subunit (SSU) ribosomal RNA (rRNA) gene that was 100% identical to that of Blastocystis infected in schoolchildren. Our information supports the evidence of water-borne transmission in this population.

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